FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15543
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTATACGACGTAGTATATCTCTGCGCGATCTCGGCTTTGAGGGGGCCT3262.0974071929485945No Hit
CGCTATACGACGTAGTATATCTGGGGGAAGAATATCAACAGGAACAGCAG1701.093739947243132No Hit
CGCTATACGACGTAGTATATCTAGGCCAAGGGTGTTGCCTCTTCCTTTTA1370.8814257221900533No Hit
CGCTATACGACGTAGTATATCTGAGGGAAGAATATCAACAGGAACAGCAG1220.784919256256836No Hit
CGCTATACGACGTAGTATATCTGGGGGGGGAAAATGGACGAAGGACAAGG1070.6884127903236184No Hit
CGCTATACGACGTAGTATATCTAACCGAGGTCGAAACGTACGTTCTTTCT1040.6691114971369748No Hit
CGCTATACGACGTAGTATATCTGGGGGCAATCAGTTTCTGGATGTGCTCT850.546869973621566No Hit
CGCTATACGACGTAGTATATCTCGGCGCGATCTCGGCTTTGAGGGGGCCT600.3860258637328701No Hit
CGCTATACGACGTAGTATATCTGAGGGGAGAATATCAACAGGAACAGCAG590.37959209933732224No Hit
CGCTATACGACGTAGTATATCTACGCGTGATCAGTAGAAACAAGGGTGTT560.36029080615067877No Hit
CGCTATACGACGTAGTATATCTGAGGGAGGAATATCAACAGGAACAGCAG530.3409895129640353No Hit
CGCTATACGACGTAGTATATCTGAGAGGCCAAGGGTGTTGCCTCTTCCTT500.32168821977739176No Hit
CGCTATACGACGTAGTATATCTCGGGACAGAAATTTGAGGAAATAAGGTG470.30238692659074823No Hit
CGCTATACGACGTAGTATATCTGGAGGGGGAAAATGGACGAAGGACAAGG460.2959531621952004No Hit
CGCTATACGACGTAGTATATCTCGAACAAATAACATTTATGCAAGCCTTA450.2895193977996526No Hit
CGCTATACGACGTAGTATATCTGGGGACAGAAATTTGAGGAAATAAGGTG430.27665186900855687No Hit
CGCTATACGACGTAGTATATCTAGGGGAAGAATATCAACAGGAACAGCAG370.2380492826352699No Hit
CGCTATACGACGTAGTATATCTAAGGGAAGAATATCAACAGGAACAGCAG360.23161551823972204No Hit
CGCTATACGACGTAGTATATCTGCGCGATCTCGGCTTTGAGGGGGCCTGA360.23161551823972204No Hit
CGCTATACGACGTAGTATATCTGGGAGCCGAACAACATGGATAGAGCAGT360.23161551823972204No Hit
CGCTATACGACGTAGTATATCTGGGACGGAAATTTGAGGAAATAAGGTGG350.22518175384417422No Hit
CGCTATACGACGTAGTATATCTCGAGGCCAAGGGTGTTGCCTCTTCCTTT350.22518175384417422No Hit
CGCTATACGACGTAGTATATCTCGGCTTTGAGGGGGCCTGACGGGATGAT340.21874798944862642No Hit
CGCTATACGACGTAGTATATCTGGGAGGCCAAGGGTGTTGCCTCTTCCTT340.21874798944862642No Hit
CGCTATACGACGTAGTATATCTCCAACACCATGTCAAGCTTTCAGGTAGA330.21231422505307856No Hit
CGCTATACGACGTAGTATATCTCAGGAGGGAAGAATATCAACAGGAACAG320.20588046065753074No Hit
CGCTATACGACGTAGTATATCTCAGGGAAGAATATCAACAGGAACAGCAG320.20588046065753074No Hit
CGCTATACGACGTAGTATATCTCGGGGAAGAATATCAACAGGAACAGCAG310.1994466962619829No Hit
CGCTATACGACGTAGTATATCTACGCGTGATCAGCAAAAGCAGGTAGATA300.19301293186643506No Hit
CGCTATACGACGTAGTATATCTGAAACGAGAAAGCTCTTATCTCTTGTTC290.1865791674708872No Hit
CGCTATACGACGTAGTATATCTAACCGAGGTCGAAACGTACGTTCTTTCC290.1865791674708872No Hit
CGCTATACGACGTAGTATATCTCAGAGGCCAAGGGTGTTGCCTCTTCCTT290.1865791674708872No Hit
CGCTATACGACGTAGTATATCTGAAACGGGAAAGCTCTTATCTCTTGTTC270.17371163867979156No Hit
CGCTATACGACGTAGTATATCTGAGGCCAAGGGTGTTGCCTCTTCCTTTT270.17371163867979156No Hit
CGCTATACGACGTAGTATATCTAGGGACAGAAATTTGAGGAAATAAGGTG270.17371163867979156No Hit
CGCTATACGACGTAGTATATCTGGGGGCCGAACAACATGGATAGAGCAGT270.17371163867979156No Hit
CGCTATACGACGTAGTATATCTCGGCTCTGAGGGGGCCTGACGGGATGAT270.17371163867979156No Hit
CGCTATACGACGTAGTATATCTGAGGGGGGAAAATGGACGAAGGACAAGG260.1672778742842437No Hit
CGCTATACGACGTAGTATATCTCCGAGGCCAAGGGTGTTGCCTCTTCCTT260.1672778742842437No Hit
CGCTATACGACGTAGTATATCTGGGGGCAGAAATTTGAGGAAATAAGGTG250.16084410988869588No Hit
CGCTATACGACGTAGTATATCTGACGGGATGATAGAAAGAACGTACGTTT240.15441034549314803No Hit
CGCTATACGACGTAGTATATCTAGGACAGAAATTTGAGGAAATAAGGTGG240.15441034549314803No Hit
CGCTATACGACGTAGTATATCTGGGGGAGGAATATCAACAGGAACAGCAG230.1479765810976002No Hit
CGCTATACGACGTAGTATATCTGGGGGACAAAAACATAATGGACTCCAAC230.1479765810976002No Hit
CGCTATACGACGTAGTATATCTGCAACAGGAACAGCAGAGTGCTGTGGAT230.1479765810976002No Hit
CGCTATACGACGTAGTATATCTCGGACAGAAATTTGAGGAAATAAGGTGG230.1479765810976002No Hit
CGCTATACGACGTAGTATATCTGGGACAGAAATTTGAGGAAATAAGGTGG230.1479765810976002No Hit
CGCTATACGACGTAGTATATCTGAAGGGGCAATAGTTGGAGAAATTTCAC220.14154281670205238No Hit
CGCTATACGACGTAGTATATCTGGGCACGGTGAGCGTGAACACAAATCCT220.14154281670205238No Hit
CGCTATACGACGTAGTATATCTAGACAGAGAATAGTTTCGAACAAATAAC220.14154281670205238No Hit
CGCTATACGACGTAGTATATCTATGCGCGATCTCGGCTTTGAGGGGGCCT220.14154281670205238No Hit
CGCTATACGACGTAGTATATCTGGGGGGCCAAGGAGGTGTCACTAAGCTA220.14154281670205238No Hit
CGCTATACGACGTAGTATATCTGGGGCAGAAATTTGAGGAAATAAGGTGG210.13510905230650455No Hit
CGCTATACGACGTAGTATATCTACGCGTGATCAGTAGAAACAAGGTAGTT210.13510905230650455No Hit
CGCTATACGACGTAGTATATCTGGGGGGGAAAATGGACGAAGGACAAGGG200.1286752879109567No Hit
CGCTATACGACGTAGTATATCTCGGCCAAGGGTGTTGCCTCTTCCTTTTA200.1286752879109567No Hit
CGCTATACGACGTAGTATATCTGGTGGGGAGCGCCAGGATGCCACAGAAA200.1286752879109567No Hit
CGCTATACGACGTAGTATATCTTGCACCTGATATTGTGGATTACTGATCG200.1286752879109567No Hit
CGCTATACGACGTAGTATATCTACAGGAACAGCAGAGTGCTGTGGATGTT200.1286752879109567No Hit
CGCTATACGACGTAGTATATCTGGGACCCGAACAACATGGATAGAGCAGT200.1286752879109567No Hit
CGCTATACGACGTAGTATATCTGGGGGGAGAATATCAACAGGAACAGCAG200.1286752879109567No Hit
CGCTATACGACGTAGTATATCTGGGACAGGAATTTGAGGAAATAAGGTGG190.12224152351540886No Hit
CGCTATACGACGTAGTATATCTGAGGGGGGAATATCAACAGGAACAGCAG180.11580775911986102No Hit
CGCTATACGACGTAGTATATCTGGCGAGGCAATTGGGACAGAAATTTGAG180.11580775911986102No Hit
CGCTATACGACGTAGTATATCTGGGGGAGCAATTGGGACAGAAATTTGAG180.11580775911986102No Hit
CGCTATACGACGTAGTATATCTGGGGGAGCAATAGTTGGAGAAATTTCAC180.11580775911986102No Hit
CGCTATACGACGTAGTATATCTGGGCCAAGGGTGTTGCCTCTTCCTTTTA180.11580775911986102No Hit
CGCTATACGACGTAGTATATCTGGGGCCCGAACAACATGGATAGAGCAGT180.11580775911986102No Hit
CGCTATACGACGTAGTATATCTCCACGCGTGATCAGTAGAAACAAGGGTG180.11580775911986102No Hit
CGCTATACGACGTAGTATATCTCAGGACAGAAATTTGAGGAAATAAGGTG170.10937399472431321No Hit
CGCTATACGACGTAGTATATCTGGGACCGGAACAACATGGATAGAGCAGT170.10937399472431321No Hit
CGCTATACGACGTAGTATATCTCGGGAGGGAAGAATATCAACAGGAACAG170.10937399472431321No Hit
CGCTATACGACGTAGTATATCTGAGGAAGGAACAATAGTTGGAGAAATTT170.10937399472431321No Hit
CGCTATACGACGTAGTATATCTGGACAGAGAATAGTTTCGAACAAATAAC170.10937399472431321No Hit
CGCTATACGACGTAGTATATCTGGCAGGGGAAAACAAAAGCAACAAAAAT160.10294023032876537No Hit
CGCTATACGACGTAGTATATCTGGGGTGGAGTCTGCTGTCCTGAGAGGAT160.10294023032876537No Hit
CGCTATACGACGTAGTATATCTGAGTCCATGAGGGAAGAATATCAACAGG160.10294023032876537No Hit
CGCTATACGACGTAGTATATCTGGCAGAGCAATTGGGACAGAAATTTGAG160.10294023032876537No Hit
CGCTATACGACGTAGTATATCTGAGGAAGGAGCAATAGTTGGAGAAATTT160.10294023032876537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC156.494369E-495.02
CGCTAGA156.494369E-495.01
AGACGAC156.494369E-495.05
GACGACG156.494369E-495.06
TAGACGA156.494369E-495.04
TATACGA15300.094.6895454
ATACGAC15200.094.68755
TACGACG15100.094.6854256
CGCTATA15400.094.383121
CTATACG15350.094.381113
GCTATAC15350.094.381112
CGACGTA15200.094.3758
ACGACGT15200.094.3757
GACGTAG15400.093.4577949
CTAGACG200.00203556971.253
ATCTCGA452.1100277E-1047.50000418-19
TATCTAA850.047.50000418-19
GACAAGG452.1100277E-1047.50000444-45
ATCTGGC452.1100277E-1047.50000418-19
ATCTGCA452.1100277E-1047.50000418-19
Produced by FastQC (version 0.11.5)