FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_309.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_309.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31355
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAGTCTTCGAGCTCTCGGAC2780.8866209535959177No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAAATGGACGAAGGACAAGG1890.6027746770849944No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTGTTT1790.5708818370275873No Hit
CGTAGTATCTATAGTACTGCTAGGGGGAAGAATATCAACAGGAACAGCAG1400.4464997608036996No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGCAAAAACATAATGGACTCCAAC1350.430553340774996No Hit
CGTAGTATCTATAGTACTGCTAACGCGAAGTAGGTACAGATGCAATTGTC970.309360548556849No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGGTGACAA970.309360548556849No Hit
CGTAGTATCTATAGTACTGCTAGGGGACAGAAATTTGAGGAAATAAGGTG850.27108914048796046No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGAAAATGGACGAAGGACAAGGGT820.26152128847073836No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGCAAAAACATAATGGACTCCAA790.2519534364535162No Hit
CGTAGTATCTATAGTACTGCTAGGGGCGGGAAGTCTTCGAGCTCTCGGAC740.23600701642481262No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGACAAAAACATAATGGACTCCAA740.23600701642481262No Hit
CGTAGTATCTATAGTACTGCTAGGGGGACAAAAACATAATGGACTCCAAC660.21049274437888693No Hit
CGTAGTATCTATAGTACTGCTAGGGGGCCTGACGGGATGATAGAAAGAAC650.20730346037314626No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGTAGATAT640.20411417636740553No Hit
CGTAGTATCTATAGTACTGCTAGAAGGTTAACAGAGTGTGTTACTGTTAC610.19454632435018337No Hit
CGTAGTATCTATAGTACTGCTAGAGGGAAGAATATCAACAGGAACAGCAG600.19135704034444267No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAAACAAAAGCAACAAAAAT600.19135704034444267No Hit
CGTAGTATCTATAGTACTGCTAAATCGCTTGCGGATATGCCAAAGGAAAC600.19135704034444267No Hit
CGTAGTATCTATAGTACTGCTAAGCCCATACACACAAGCAGGCAGGCAGG590.18816775633870195No Hit
CGTAGTATCTATAGTACTGCTACAGAGCAGAAATGAAAAGTGGCGAGAGC560.17859990432147982No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGCAATCAGTTTCTGGATGTGCT550.17541062031573912No Hit
CGTAGTATCTATAGTACTGCTAGAGGGGGGAAAATGGACGAAGGACAAGG540.17222133630999842No Hit
CGTAGTATCTATAGTACTGCTACGGGAGGGAAGAATATCAACAGGAACAG500.15946420028703556No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGTAGTTT480.15308563227555413No Hit
CGTAGTATCTATAGTACTGCTACCTCCAGAGCAGAAATGAAAAGTGGCGA470.14989634826981343No Hit
CGTAGTATCTATAGTACTGCTAGCGGGAGTTTTGTTCAGCATCCAGAACT470.14989634826981343No Hit
CGTAGTATCTATAGTACTGCTATTGGGACAGAAATTTGAGGAAATAAGGT470.14989634826981343No Hit
CGTAGTATCTATAGTACTGCTAGGGGGACGAAAACATAATGGACTCCAAC460.1467070642640727No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGAAAATGGACGAAGGACAAGGG450.143517780258332No Hit
CGTAGTATCTATAGTACTGCTAGGGGGCAGAAATTTGAGGAAATAAGGTG450.143517780258332No Hit
CGTAGTATCTATAGTACTGCTACGGGGAAGAATATCAACAGGAACAGCAG440.1403284962525913No Hit
CGTAGTATCTATAGTACTGCTACCCCTCTTAGTTTGCATAGTTTCCCGTT440.1403284962525913No Hit
CGTAGTATCTATAGTACTGCTACCGCGAAGTAGGTACAGATGCAATTGTC430.13713921224685058No Hit
CGTAGTATCTATAGTACTGCTACGGGACAGAAATTTGAGGAAATAAGGTG420.13394992824110988No Hit
CGTAGTATCTATAGTACTGCTACGAGATCGGAAGAGCACACGTCTGAACT420.13394992824110988Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTAGTATCTATAGTACTGCTAGGGGGGAGAATATCAACAGGAACAGCAG420.13394992824110988No Hit
CGTAGTATCTATAGTACTGCTAGGGACAGAAATTTGAGGAAATAAGGTGG420.13394992824110988No Hit
CGTAGTATCTATAGTACTGCTATGCGGCACAGATTGAAAGCGACAGAGAA420.13394992824110988No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGCAATAGTTGGAGAAATTTCAC400.12757136022962845No Hit
CGTAGTATCTATAGTACTGCTAAGGCGAACTTCAGTGTAATCTTCAACCG400.12757136022962845No Hit
CGTAGTATCTATAGTACTGCTAGGGGCGGGGAGTCTTCGAGCTCTCGGAC400.12757136022962845No Hit
CGTAGTATCTATAGTACTGCTACCGAGGCCAAGGGTGTTGCCTCTTCCTT390.12438207622388774No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGGGAAAAC390.12438207622388774No Hit
CGTAGTATCTATAGTACTGCTAGGGGGCAAAAACATAATGGACTCCAACA390.12438207622388774No Hit
CGTAGTATCTATAGTACTGCTAGCGAGGCCAAGGGTGTTGCCTCTTCCTT380.12119279221814704No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGAGTTTAA380.12119279221814704No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTATTT380.12119279221814704No Hit
CGTAGTATCTATAGTACTGCTAAGGGACAGAAATTTGAGGAAATAAGGTG370.11800350821240631No Hit
CGTAGTATCTATAGTACTGCTAGCGGGGGAAAACAAAAGCAACAAAAATG360.1148142242066656No Hit
CGTAGTATCTATAGTACTGCTAAGCGGGGGAAAACAAAAGCAACAAAAAT350.1116249402009249No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGAAAACAAAAGCAACAAAAATG350.1116249402009249No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAACAGTGACCACAGAAGCTG340.1084356561951842No Hit
CGTAGTATCTATAGTACTGCTACAGAGGCCAAGGGTGTTGCCTCTTCCTT340.1084356561951842No Hit
CGTAGTATCTATAGTACTGCTAGGGAGGCCAAGGGTGTTGCCTCTTCCTT340.1084356561951842No Hit
CGTAGTATCTATAGTACTGCTACAGGAGGGAAGAATATCAACAGGAACAG330.10524637218944347No Hit
CGTAGTATCTATAGTACTGCTAGCGAGGGCAATTGGGACAGAAATTTGAG320.10205708818370277No Hit
CGTAGTATCTATAGTACTGCTAGCGAGAGGAATTGGGACAGAAATTTGAG320.10205708818370277No Hit
CGTAGTATCTATAGTACTGCTAGGGGAGGGAAGAATATCAACAGGAACAG320.10205708818370277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT156.5658387E-494.9696962
CGTAGTA30600.094.495221
CTATAGT30950.094.2025769
GTATCTA30900.094.201335
TCTATAG30900.094.201338
AGTATCT30900.094.201334
ATCTATA30850.094.200087
TATCTAT30850.094.200086
TAGTATC30800.094.198843
GTAGTAT30650.094.195072
CATAGTA250.004919485757.1641581
CTTTTAC205.3154194E-447.56070380-81
TGTGAGA205.3154194E-447.56070380-81
ACTGTAG205.3154194E-447.56070392-93
CCGTGCC205.3154194E-447.56070388-89
TGACCGT205.3154194E-447.56070384-85
CAGTACA205.3154194E-447.56070386-87
GGTCTGA205.3154194E-447.56070384-85
TGCTGGT205.3154194E-447.56070380-81
ACCGTGC205.3154194E-447.56070386-87
Produced by FastQC (version 0.11.5)