FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77469
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATACAGAGTAGTACAGACCGGCGCTGGAGCTAGGATGAGTCCCAAT6350.8196827117943952No Hit
CGACATACAGAGTAGTACAGACCGGCACTGGAGCTAGGATGAGTCCCAAT4730.6105668073681085No Hit
CGACATACAGAGTAGTACAGACGCGTGATCAGTAGAAACAAGGGTGTTTT2420.3123830177232183No Hit
CGACATACAGAGTAGTACAGACAGGCCCCCTCAAAGCCGAGATCGCGCAG2220.2865662393989854No Hit
CGACATACAGAGTAGTACAGACCAGCGCTGGAGCTAGGATGAGTCCCAAT1950.25171358866127097No Hit
CGACATACAGAGTAGTACAGACGGGGGGGGAACAGTGACCACAGAAGCTG1830.2362235216667312No Hit
CGACATACAGAGTAGTACAGACCAGCACTGGAGCTAGGATGAGTCCCAAT1590.2052433876776517No Hit
CGACATACAGAGTAGTACAGACAGGCGCTGGAGCTAGGATGAGTCCCAAT1570.2026617098452284No Hit
CGACATACAGAGTAGTACAGACGGGGGGGGAACGCCAGGATGCCACAGAA1510.19491667634795853No Hit
CGACATACAGAGTAGTACAGACAGGCGAACAGAGGTTATAAGAATGATGG1170.15102815319676258No Hit
CGACATACAGAGTAGTACAGACCGGCCCTGGAGCTAGGATGAGTCCCAAT950.12262969704010637No Hit
CGACATACAGAGTAGTACAGACAGCAGAGGCCATGGAGGTTGCTAATCAG900.11617550245904813No Hit
CGACATACAGAGTAGTACAGACGCGTGATCAGTAGAAACAAGGTAGTTTT890.1148846635428365No Hit
CGACATACAGAGTAGTACAGACCCGCGCTGGAGCTAGGATGAGTCCCAAT870.11230298571041321No Hit
CGACATACAGAGTAGTACAGACGGGGGCAGAAATTTGAGGAAATAAGGTG870.11230298571041321No Hit
CGACATACAGAGTAGTACAGACCTGCGCTGGAGCTAGGATGAGTCCCAAT800.10326711329693167No Hit
CGACATACAGAGTAGTACAGACCGGCGCCAAACTCTCCTTATTTCTTCTT800.10326711329693167No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAGT201.7337099E-594.998067
CGACATA77950.092.190781
CATACAG78650.091.797234
ACATACA78500.091.791123
ACAGAGT78500.091.60967
CAGAGTA78450.091.6074458
TACAGAG79200.091.51966
AGAGTAG78700.091.4975059
ATACAGA79150.091.457395
GACATAC79050.091.0923842
GACATAT255.244852E-575.998452
TATAGAG255.244852E-575.998456
TGACATA200.00205107871.38681
CATATAG301.2937405E-463.3320434
AACATAC250.005004062356.9988372
AGAGGAG654.348658E-851.1528059
GACAGCC650.047.49903520-21
GACAGTA252.8159298E-547.4990320-21
GGAATCT205.374207E-447.4990328-29
CAGCGGG205.374207E-447.4990322-23
Produced by FastQC (version 0.11.5)