FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78226
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTACACGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC928211.865620126300719TruSeq Adapter, Index 5 (100% over 35bp)
CGCTACACGCGTCGAGATCACGAGGCCAAGGGTGTTGCCTCTTCCTTTTA4520.5778130033492701No Hit
CGCTACACGCGTCGAGATCACGCGTGATCAGTAGAAACAAGGGTGTTTTT4450.5688645718814717No Hit
CGCTACACGCGTCGAGATCACGAGGGGGCCTGACGGGATGATAGAAAGAA2850.36432899547465036No Hit
CGCTACACGCGTCGAGATCACGGGGGCCTGACGGGATGATAGAAAGAACG2810.35921560606447983No Hit
CGCTACACGCGTCGAGATCACGCAAAGAGGGCCTATTATCTTTTGCCTAG1560.19942218699665074No Hit
CGCTACACGCGTCGAGATCACGCGGCCAAGGGTGTTGCCTCTTCCTTTTA1390.177690282003426No Hit
CGCTACACGCGTCGAGATCACGGGGGGGGGAAAATGGACGAAGGACAAGG1370.1751335872983407No Hit
CGCTACACGCGTCGAGATCACGGAGGGGGGAAAATGGACGAAGGACAAGG1300.16618515583054227No Hit
CGCTACACGCGTCGAGATCACGGAAACCACTGACTGTCCAGCAGCAAAGT1240.15851507171528648No Hit
CGCTACACGCGTCGAGATCACGGGGCCAAGGGTGTTGCCTCTTCCTTTTA1180.15084498760003068No Hit
CGCTACACGCGTCGAGATCACGGGGGGAAGAATATCAACAGGAACAGCAG1130.14445325083731753No Hit
CGCTACACGCGTCGAGATCACGCAAAGCAGGGTGACAAAAACATAATGGA1070.13678316672206173No Hit
CGCTACACGCGTCGAGATCACGGAGGGGGCCTGACGGGATGATAGAAAGA1040.13294812466443381No Hit
CGCTACACGCGTCGAGATCACGAAAAGCAGGGTGACAAAAACATAATGGA1040.13294812466443381No Hit
CGCTACACGCGTCGAGATCACGGTGAGCGTGAACACAAATCCTAAAATTC950.12144299849155013No Hit
CGCTACACGCGTCGAGATCACGGGGGACAGAAATTTGAGGAAATAAGGTG940.12016465113900748No Hit
CGCTACACGCGTCGAGATCACGCCCCCACGCGTGATCAGTAGAAACAAGG900.11505126172883695No Hit
CGCTACACGCGTCGAGATCACGGGGGGGCCTGACGGGATGATAGAAAGAA870.11121621967120907No Hit
CGCTACACGCGTCGAGATCACGCAGAGACTGGAAAGTGTCTTTGCAGGAA860.10993787231866642No Hit
CGCTACACGCGTCGAGATCACGGTGGGGAGCGCCAGGATGCCACAGAAAT850.1086595249661238No Hit
CGCTACACGCGTCGAGATCACGCGTGATCAGTAGAAACAAGGTAGTTTTT850.1086595249661238No Hit
CGCTACACGCGTCGAGATCACGGGGGGCCTGACGGGATGATAGAAAGAAC790.10098944085086799No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACA156.545025E-495.180651
TACACGG156.595213E-494.9980854
CGGGTCG156.595213E-494.9980858
GCGTCGA77650.094.753399
CGCGTCG77650.094.692228
ACGCGTC77700.094.6312947
CGCTACA77700.094.445671
GCTACAC78050.094.328662
TACACGC77850.094.3269354
CTACACG78100.094.268273
ACACGCG78100.094.207455
CACGCGT79800.092.021946
GGGTCGA200.002066999271.248569
GACACGC200.002066999271.248564
ACGGGTC200.002066999271.248567
CGCGACA250.00496631857.108391
CGACACG250.005004239756.9988483
CGCTGGG252.8160532E-547.49904320-21
ACGCCGG452.237357E-1047.49904320-21
ACGCCGC301.484168E-647.49904320-21
Produced by FastQC (version 0.11.5)