FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_295.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_295.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14442
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACGATACACGCGTATGTACGGCGCCATCAAGGGAGCAATTTTACAA970.6716521257443567No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAAGAATACTGACATCAGAG900.6231823847112589No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAGAATACTGACATCAGAGT890.6162581359922448No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAGAATACTGACATCAGAGT710.49162165904999305No Hit
CGATACGATACACGCGTATGTAGGAACAGGATACACCATGGACACAGTAA470.3254396897936574No Hit
CGATACGATACACGCGTATGTAGCAAGGGGACGTAGTGTTGGTAATGAAA470.3254396897936574No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAACAAAATAACATTCGAAG460.3185154410746434No Hit
CGATACGATACACGCGTATGTAGGGACAGAAATTTGAGGAAATAAGGTGG450.31159119235562943No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAAAACATAATGGACTCCAA430.2977426949176014No Hit
CGATACGATACACGCGTATGTAGGGGACAGAAATTTGAGGAAATAAGGTG420.29081844619858743No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAAACAAAAGCAACAAAAAT420.29081844619858743No Hit
CGATACGATACACGCGTATGTAGGGCAAAGAAGACAAGAGATAAGCATCA410.28389419747957345No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAATAGCAATAAGACCCAAA400.27696994876055947No Hit
CGATACGATACACGCGTATGTAGAGGAAGCAAAATTAAACAGAGAAGAAA340.23542445644647556No Hit
CGATACGATACACGCGTATGTAGGGCAAAGAAGACAAGAGATATGGCCCA310.21465171028943358No Hit
CGATACGATACACGCGTATGTAGGGGGCCTGACGGGATGATAGAAAGAAC300.2077274615704196No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAGGAGGTGTCACTAAGCTA300.2077274615704196No Hit
CGATACGATACACGCGTATGTAGGGGGGCAAGAATACTGACATCAGAGTC290.2008032128514056No Hit
CGATACGATACACGCGTATGTACGGCGCAAAGAGGGCCTATTATCTTTTG290.2008032128514056No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAAAAACATAATGGACTCCA280.19387896413239164No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAATAGTTGGAGAAATTTCAC280.19387896413239164No Hit
CGATACGATACACGCGTATGTAAACAGAACACACCAATACTCAGAAAAGA270.18695471541337766No Hit
CGATACGATACACGCGTATGTAGGGGAAGCAAAATTAAACAGAGAAGAAA260.18003046669436365No Hit
CGATACGATACACGCGTATGTACGCCGCCATCAAGGGAGCAATTTTACAA250.17310621797534967No Hit
CGATACGATACACGCGTATGTAAGGCGCCATCAAGGGAGCAATTTTACAA240.1661819692563357No Hit
CGATACGATACACGCGTATGTAGGGAGAGCAATTGGGACAGAAATTTGAG230.1592577205373217No Hit
CGATACGATACACGCGTATGTAGGGGGAGCAATAGTTGGAGAAATTTCAC220.15233347181830773No Hit
CGATACGATACACGCGTATGTAGGGGGGCAAAAACATAATGGACTCCAAC220.15233347181830773No Hit
CGATACGATACACGCGTATGTACAGGAGGGAAGAATATCAACAGGAACAG210.14540922309929372No Hit
CGATACGATACACGCGTATGTAGGGGGCCAAGGAGGTGTCACTAAGCTAT210.14540922309929372No Hit
CGATACGATACACGCGTATGTAGGGGGGCCAAGGAGGTGTCACTAAGCTA210.14540922309929372No Hit
CGATACGATACACGCGTATGTACGGCCGGAAATAGCAATAAGACCCAAAG210.14540922309929372No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAATAGTTGGAGAAATTTCA210.14540922309929372No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAACAAAAGCAACAAAAATG200.13848497438027974No Hit
CGATACGATACACGCGTATGTACGGCAATCGCACGCAAAGAGGGCCTATT190.13156072566126575No Hit
CGATACGATACACGCGTATGTAGCGAGAGCAATTGGGACAGAAATTTGAG190.13156072566126575No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAATAGTTGGAGAAATTTCAC190.13156072566126575No Hit
CGATACGATACACGCGTATGTAGGGAAGGCAAGAGAAGAACCCCGCACTC190.13156072566126575No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAATACCACTATATGATGCAA190.13156072566126575No Hit
CGATACGATACACGCGTATGTAGGGGGGGAAAACAAAAGCAACAAAAATG180.12463647694225177No Hit
CGATACGATACACGCGTATGTAGGTACAGATGCAATTGTCGTTCTAAGTG170.11771222822323778No Hit
CGATACGATACACGCGTATGTAAGGGACAGAAATTTGAGGAAATAAGGTG170.11771222822323778No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAACAGTGACCACAGAAGCTG170.11771222822323778No Hit
CGATACGATACACGCGTATGTAAGGAAGCAAAATTAAACAGAGAAGAAAT170.11771222822323778No Hit
CGATACGATACACGCGTATGTAGGGGGACAAAAACATAATGGACTCCAAC170.11771222822323778No Hit
CGATACGATACACGCGTATGTACGGCCCCAAATAGGCCTCTAGATTGAAT170.11771222822323778No Hit
CGATACGATACACGCGTATGTACGGCCAGCAATGTTACATTTACCCAAAT160.1107879795042238No Hit
CGATACGATACACGCGTATGTAGGGGGGGAAATAGCAATAAGACCCAAAG160.1107879795042238No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAAGAGAAGAACCCCGCACT160.1107879795042238No Hit
CGATACGATACACGCGTATGTAGGAAGCAAAATTAAACAGAGAAGAAATA160.1107879795042238No Hit
CGATACGATACACGCGTATGTAGGGGGGGAAACAAAATAACATTCGAAGC160.1107879795042238No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAATATATTCAATATGGAGA160.1107879795042238No Hit
CGATACGATACACGCGTATGTACAGAGCAGAAATGAAAAGTGGCGAGAGC160.1107879795042238No Hit
CGATACGATACACGCGTATGTAGGCAAGGGGACGTAGTGTTGGTAATGAA150.1038637307852098No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAAATAACATTCGAAGCAAC150.1038637307852098No Hit
CGATACGATACACGCGTATGTAGGGGGCGGAAATAGCAATAAGACCCAAA150.1038637307852098No Hit
CGATACGATACACGCGTATGTAGGGAAGCAAAATTAAACAGAGAAGAAAT150.1038637307852098No Hit
CGATACGATACACGCGTATGTAAGGAAGGCAAGAGAAGAACCCCGCACTC150.1038637307852098No Hit
CGATACGATACACGCGTATGTAGAGGGAAGAATATCAACAGGAACAGCAG150.1038637307852098No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAAGGAGGTGTCACTAAGCT150.1038637307852098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACGC201.6860606E-594.996549
GATGCAC201.6860606E-594.996547
TACGATG201.6860606E-594.996544
CGATGCA201.6860606E-594.996546
CGATACA13950.094.996536
GATACGA14200.094.996532
TACACGC13850.094.996539
TACGATA13950.094.996534
ACGATAC13950.094.996535
ATACACG13900.094.996538
ATACGAT14150.094.996523
CGATACG14150.094.990681
GATACAC14050.093.982337
ATGCACG255.1013292E-575.997228
ACGATGC255.1013292E-575.997225
AAGTACA205.3015794E-447.4982736-37
CATTGAC205.3015794E-447.4982794-95
GGCGCGA205.3015794E-447.4982724-25
GTACCGC205.3015794E-447.4982720-21
GGCGCCA403.983587E-947.4982724-25
Produced by FastQC (version 0.11.5)