FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_294.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_294.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21753
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTCGCACGCGTACGACGCACTCCCATTCGCTTCTGGTAGGCCTGCAA980.4505125729784397No Hit
CGAGTCGCACGCGTACGACGCACTAAGACCACTGTGGACCATATGGCCAT830.3815565669103112No Hit
CGAGTCGCACGCGTACGACGCACCGAGGCCAAGGGTGTTGCCTCTTCCTT380.17468854870592562No Hit
CGAGTCGCACGCGTACGACGCACCGGGGCCAAGGAGGTGTCACTAAGCTA380.17468854870592562No Hit
CGAGTCGCACGCGTACGACGCAAGAAGCTCAAAAGAGAAATAACGTTCCA380.17468854870592562No Hit
CGAGTCGCACGCGTACGACGCACGGAAACCACTGACTGTCCAGCAGCAAA350.1608973474922999No Hit
CGAGTCGCACGCGTACGACGCACGCAAAGAGGGCCTATTATCTTTTGCCT300.1379120121362571No Hit
CGAGTCGCACGCGTACGACGCAAAGAGAACACAATCTGGACTAGCGGGAG300.1379120121362571No Hit
CGAGTCGCACGCGTACGACGCACCCCCATTCGCTTCTGGTAGGCCTGCAA280.12871787799383994No Hit
CGAGTCGCACGCGTACGACGCAAGCATCAATGAACTGAGCAATCTTGCAA270.12412081092263137No Hit
CGAGTCGCACGCGTACGACGCAAGGGGACGTAGTGTTGGTAATGAAACGA260.1195237438514228No Hit
CGAGTCGCACGCGTACGACGCACGGGGGCCAAGGAGGTGTCACTAAGCTA240.11032960970900565No Hit
CGAGTCGCACGCGTACGACGCACCCCCCACGCGTGATCAGTAGAAACAAG240.11032960970900565No Hit
CGAGTCGCACGCGTACGACGCACGGAGGCCAAGGGTGTTGCCTCTTCCTT240.11032960970900565No Hit
CGAGTCGCACGCGTACGACGCACAGAGCAGAAATGAAAAGTGGCGAGAGC240.11032960970900565No Hit
CGAGTCGCACGCGTACGACGCAGGGGACAGAAATTTGAGGAAATAAGGTG230.10573254263779709No Hit
CGAGTCGCACGCGTACGACGCAATTGGGACAGAAATTTGAGGAAATAAGG220.10113547556658853No Hit
CGAGTCGCACGCGTACGACGCAGGGGCCCGAACAACATGGATAGAGCAGT220.10113547556658853No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA156.5308437E-494.99779
CACGCAT156.5308437E-494.99778
ACGCGTA21450.094.5548259
GAGTCGC21850.094.128152
AGTCGCA21850.094.128153
CGAGTCG21800.094.124651
GTCGCAC21750.094.124164
TCGCACG21700.094.122145
GCACGCG22000.092.406857
CGCACGC22750.089.986846
CACGCGT22800.088.9561848
ACGCATC357.68905E-847.49885618-19
ACGCAAT357.68905E-847.49885618-19
GCGCGTG357.68905E-847.49885624-25
GCACGAG357.68905E-847.49885620-21
GCACCGC357.68905E-847.49885620-21
CGCACTG357.68905E-847.49885618-19
CGCACCG1050.047.49885618-19
CGCACCA700.047.49885618-19
CGCAAGG1050.047.49885618-19
Produced by FastQC (version 0.11.5)