FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGCGACATACGTCGTGTCAGGCCCCCTCAAAGCCGAGATCGCGCAG60.5565862708719851No Hit
CGCGCGCGACATACGTCGTGTCAGTAGAAACAAGGTAGTTTTTTACTCTA50.463821892393321No Hit
CGCGCGCGACATACGTCGTGTCCGTAGAAACAAGGTAGTTTTTTACTCTA50.463821892393321No Hit
CGCGCGCGACATACGTCGTGTCAGGATATAGCGGGAGTTTTGTTCAGCAT50.463821892393321No Hit
CGCGCGCGACATACGTCGTGTCCGCCCCCCTTCAATGAAGCCGGCAATGG40.3710575139146568No Hit
CGCGCGCGACATACGTCGTGTCCTACGCGTGATCAGCAAAAGCAGGTAGA40.3710575139146568No Hit
CGCGCGCGACATACGTCGTGTCCGGCCCCCTCAAAGCCGAGATCGCGCAG40.3710575139146568No Hit
CGCGCGCGACATACGTCGTGTCGAGGCCAAGGGTGTTGCCTCTTCCTTTT30.27829313543599254No Hit
CGCGCGCGACATACGTCGTGTCCGGCCCCCTCAAAGCCGAGATCGGAAGA30.27829313543599254No Hit
CGCGCGCGACATACGTCGTGTCCAGAGCAGAAATGAAAAGTGGCGAGAGC30.27829313543599254No Hit
CGCGCGCGACATACGTCGTGTCCAGAGGCGAATGGGAGTGCAGATGCAGC30.27829313543599254No Hit
CGCGCGCGACATACGTCGTGTCCGGGGGCCAAGGAGGTGTCACTAAGCTA30.27829313543599254No Hit
CGCGCGCGACATACGTCGTGTCAGGCCCCCTCAAAGCCGAGATCGGAAGA30.27829313543599254No Hit
CGCGCGCGACATACGTCGTGTCCGGGAGGGAAGAATATCAACAGGAACAG30.27829313543599254No Hit
CGCGCGCGACATACGTCGTGTCAGGCGCCCTCAAAGCCGAGATCGCGCAG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCCGCGTGATCAGTAGAAACAAGGTAGTT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCTCCAAGGGATGTGCAGTTGCGACGGCAT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGTAGAAACAAGGTACTTTTTTGGACAG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCGGCGGGGCAAGGAGGTGTCACTAAGCTA20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGTAGAAACAAGGTCGTTTTTAAACAAT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCAGGGAGCAAATGGAGTAAAAGGATTTT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCCCACCAAAACATATGCATCTGCATTCT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCCCGGGGCAACATCTTCTCCTATTTCAT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGCAAAAGCAGGGTGACAAAAACATAAT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCGGGGCCAAGGAGGTGTCACTAAGCTAT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCACGCACCCTCAAAGCCGAGATCGCGCAG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCGGGAGCGAATGGGAGTGCAGATGCAGC20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCTAGGGAAGAATATCAACAGGAACAGCAG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGTACAAACAAGGTAGTTTTTTACTCTA20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCCCCCACGCGTGATCAGTAGAAACAAGG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCTGGACAGTGGTGAACAGTATATGTAAC20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGCAAAAGCAGGTGTTTAAAATGAATCC20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCGGGAGGGGAACAGTGACCACAGAAGCTG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCGAACGCGAAGTAGGTACAGATGCAATT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCGGCCAAAAGCATGGAATATGATGCCGT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCGGGGGGCCAAGGAGGTGTCACTAAGCTA20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCGGGGGAACAAGGTCGTTTTTAAACAATT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCGGGCCCCCTCAAAGCCGAGATCGCGCAG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGTAGCAACAAGGCAGTTTTTTACTCTA20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCGTAGAAACAAGGTACTTTTTTGGACAG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCGGGGACAGAAATTTGAGGAAATAAGGTG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGCAAAAGCAGGTAGATATTTAAAGATG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGGAGAAACAAGGTAGTTTTTTACTCTA20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCGGCCCCCTTCAATGAAGCCGGCAATGG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCGTAGAAACAAGGTCGTTTTTAAACAAT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCGGGGGGCAAGGAGGTGTCACTAAGCTA20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGGGAGCAAATGGAGTAAAAGGATTTTC20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGGAGAAACAAGGCAGTTTTTTACTCTA20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCGTACGCGTGATCAGTAGAAACAAGGTAG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGTAGAAACAAGGCCGTTTTTAAACAAT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCGCACAAAAACATATGCATCTGCATTCT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGAAGAAACAAGGTAGTTTTTTACTCTA20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGGGAGGGAACTTCACCAATGGGACAGC20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCAGGGGCGAAGGAGGTGTCACTAAGCTA20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCACCAACTTGGGGGGTTTTCGGCTGAATC20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGCAGAAGCAGGTTGACAAAAACATAAT20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCAGTAGCAACAAGGTACTTTTTTGGACAG20.1855287569573284No Hit
CGCGCGCGACATACGTCGTGTCCATCACTTGGTCCATCGGTAATTATGGT20.1855287569573284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACA1050.094.999995
GCGACAT1050.094.999996
GCGCGCG1050.094.999992
GCGCGAC1050.094.999994
GACATAC1050.094.999998
ACATACG1050.094.999999
CGCGCGC1050.094.999991
CGCGCGA1050.094.999993
CGACATA1050.094.999997
GTGTCCC204.24539E-447.50000418-19
CGTGTCG204.24539E-447.50000416-17
GTGTCCG150.00895845247.518-19
GTGTCAG251.9715004E-547.518-19
TGTCAGG150.00895845247.518-19
CGTGTCC457.4578566E-1147.516-17
CGTGTCA308.971565E-747.516-17
ACGTCGT1050.047.49999612-13
GTCGTGT1050.047.49999614-15
TCGTGTC1050.047.49999616-17
TACGTCG1050.047.49999612-13
Produced by FastQC (version 0.11.5)