FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_292.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_292.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52586
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGAGTCTATATACGTCGAGGCCAAGGGTGTTGCCTCTTCCTTTTA4560.8671509527250598No Hit
CGAGATGAGTCTATATACGTCGCGGCCAAGGGTGTTGCCTCTTCCTTTTA2440.4640018255809531No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGGAAAATGGACGAAGGACAAGG2110.4012474803179554No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAAATGGAGTAAAAGGATTTT1210.2300992659643251No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGGAACAGTGACCACAGAAGCTG1150.21868938500741644No Hit
CGAGATGAGTCTATATACGTCGAGGCCCAGGGTGTTGCCTCTTCCTTTTA1020.1939679762674476No Hit
CGAGATGAGTCTATATACGTCGGGGGGCTATATGAAGCAATCGAGGAGTG920.17495150800593315No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAATAGTTGGAGAAATTTCAC890.1692465675274788No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAAGATTGGTCTTGTCTTTAG840.15973833339672155No Hit
CGAGATGAGTCTATATACGTCGAGGCCCATGCAACTGGCAAGTGCACCAG810.15403339291826723No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGAAAATGGACGAAGGACAAGGG800.15213174609211577No Hit
CGAGATGAGTCTATATACGTCGGGGCCAAGGGTGTTGCCTCTTCCTTTTA780.14832845243981288No Hit
CGAGATGAGTCTATATACGTCGGGGGGGACAAGATTGGTCTTGTCTTTAG720.13691857148290418No Hit
CGAGATGAGTCTATATACGTCGGGGGCAGAAATTTGAGGAAATAAGGTGG700.13311527783060131No Hit
CGAGATGAGTCTATATACGTCGGAGGGGGGAAAATGGACGAAGGACAAGG670.12741033735214696No Hit
CGAGATGAGTCTATATACGTCGGGGGGGCAAATGGAGTAAAAGGATTTTC660.12550869052599553No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAAGGAGGTGTCACTAAGCTA600.11409880956908683No Hit
CGAGATGAGTCTATATACGTCGGGGAGGGCAATTGGGACAGAAATTTGAG590.11219716274293537No Hit
CGAGATGAGTCTATATACGTCGCGGCACAGATTGAAAGCGACAGAGAATA580.11029551591678394No Hit
CGAGATGAGTCTATATACGTCGGGGGGCAGAAATTTGAGGAAATAAGGTG550.1045905754383296No Hit
CGAGATGAGTCTATATACGTCGGGGGACAGAAATTTGAGGAAATAAGGTG530.10078728178602671No Hit
CGAGATGAGTCTATATACGTCGCCCAGCATTAAGCATCAATGAACTGAGC530.10078728178602671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGATG156.5333326E-495.1792141
AGCCTAT201.7284912E-594.998098
GGGTCTA156.583056E-494.998097
GGTGAGT156.583056E-494.998094
GCCTATA201.7284912E-594.998099
GGTCTAT156.583056E-494.998098
GTGAGTC156.583056E-494.998095
GATGAGG156.583056E-494.998094
AGGTGAG156.583056E-494.998093
AGTCTGT156.583056E-494.998098
TGAGCCT201.7284912E-594.998096
GAGATGG201.7284912E-594.998092
CGAGATG51850.094.72031
GAGATGA52000.094.5413742
GAGTCTA51550.094.168827
GTCTATA51550.093.8923959
AGTCTAT51750.093.7131048
TGAGTCT53400.090.906426
AGATGAG53950.090.860093
ATGAGTC53900.090.063135
Produced by FastQC (version 0.11.5)