FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59521
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTCTAGATACTCTGTAGAGAAAAGCGAAAGCATAGAAAGTGAGTGTG5940.997967104047311No Hit
CGCGTCTAGATACTCTGTAGAGAAAAACCAAAGTTTAATGATATGGGTCC4330.7274743367886964No Hit
CGCGTCTAGATACTCTGTAGAGAAGAGAAAGGCACCACACACTTTTAGAC2910.48890307622519785No Hit
CGCGTCTAGATACTCTGTAGAGCCCCGATTGGGCCAAAAGAACCAAAACC2850.47882260042674013No Hit
CGCGTCTAGATACTCTGTAGAGCTACCAAATGTTCGCGAATCATTGGGAA1770.2973740360545018No Hit
CGCGTCTAGATACTCTGTAGAGACCCATTAGAGCACATCCAGAAACTGAT1650.2772130844575864No Hit
CGCGTCTAGATACTCTGTAGAGAAAAGCCAAAGTTTAATGATATGGGTCC1210.2032895952688967No Hit
CGCGTCTAGATACTCTGTAGAGCTGCCAAATGTTCGCGAATCATTGGGAA1180.19824935736966784No Hit
CGCGTCTAGATACTCTGTAGAGCCGAGATCAAACCATTGCACTCCAGCCT1150.193209119470439No Hit
CGCGTCTAGATACTCTGTAGAGATCAGCAAAAGCAGGTGGCACGATGAAA1140.19152904017069605No Hit
CGCGTCTAGATACTCTGTAGAGCCAAAACCAAAGGCCTTAATCCCATTAT1110.1864888022714672No Hit
CGCGTCTAGATACTCTGTAGAGAAAGGCACCACACACTTTTAGACCACCA980.1646477713748089No Hit
CGCGTCTAGATACTCTGTAGAGGGGGGGAGAAGCTTTCAGTCTCGTACCA960.161287612775323No Hit
CGCGTCTAGATACTCTGTAGAGAACAAGGGAAGAAGAAAAGCGAAAGCAT870.14616689907763644No Hit
CGCGTCTAGATACTCTGTAGAGCACACGTCTGAACTCCAGTCACACAGTG860.14448681977789352TruSeq Adapter, Index 5 (100% over 32bp)
CGCGTCTAGATACTCTGTAGAGAAAACCAAAGTTTAATGATATGGGTCCA850.14280674047815056No Hit
CGCGTCTAGATACTCTGTAGAGGCAGGGGCAAGAGAGAGAAGAGAAAGGC810.13608642327917878No Hit
CGCGTCTAGATACTCTGTAGAGGAAAAGGGAAAGCATAGAAAGTGAGTGT800.13440634397943582No Hit
CGCGTCTAGATACTCTGTAGAGGCCAAAAGAACCAAAACCAAAGGCCTTA760.12768602678046403No Hit
CGCGTCTAGATACTCTGTAGAGAAGGGGAGAAGAAAAGCGAAAGCATAGA750.12600594748072108No Hit
CGCGTCTAGATACTCTGTAGAGCCGAGATCGCGCAGAGACTGGAAAGTGT740.12432586818097816No Hit
CGCGTCTAGATACTCTGTAGAGATCAGCAAAAGCAGGAGCAAGAGAGAGA690.1159254716822634No Hit
CGCGTCTAGATACTCTGTAGAGCAAGGGGGAAGAAAAGCGAAAGCATAGA680.11424539238252046No Hit
CGCGTCTAGATACTCTGTAGAGAACCGGGGAAGAAGTGGACGTTTGAGTG680.11424539238252046No Hit
CGCGTCTAGATACTCTGTAGAGCAGCAAAAGCAGGAGCAAGAGAGAGAAG680.11424539238252046No Hit
CGCGTCTAGATACTCTGTAGAGATCAGCAAAAGCAGGGTGACAAAAACAT680.11424539238252046No Hit
CGCGTCTAGATACTCTGTAGAGCGGGGGACAAAAACATAATGGACTCCAA680.11424539238252046No Hit
CGCGTCTAGATACTCTGTAGAGAGAAGAGAAAGGCACCACACACTTTTAG660.11088523378303455No Hit
CGCGTCTAGATACTCTGTAGAGAAGAACCAAAGTTTAATGATATGGGTCC660.11088523378303455No Hit
CGCGTCTAGATACTCTGTAGAGGTGGGCGGATCACCTGAGGTCGAGAGTT650.10920515448329161No Hit
CGCGTCTAGATACTCTGTAGAGCGGGGGAGAAGCTTTCAGTCTCGTACCA640.10752507518354867No Hit
CGCGTCTAGATACTCTGTAGAGGGCCGGGGAAGAAGTGGACGTTTGAGTG640.10752507518354867No Hit
CGCGTCTAGATACTCTGTAGAGGGGGCCTGACGGGATGATAGAAAGAACG630.1058449958838057No Hit
CGCGTCTAGATACTCTGTAGAGGTGGCACGATGAAACCTTCTAATTCTGG610.10248483728431983No Hit
CGCGTCTAGATACTCTGTAGAGGACCGGGGAAGAAGTGGACGTTTGAGTG610.10248483728431983No Hit
CGCGTCTAGATACTCTGTAGAGGGGAGAAGAAAAGCGAAAGCATAGAAAG600.10080475798457689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCT254.5009983E-795.2308351
CGCGTCT57500.095.2308351
CCCGTCT156.5235415E-495.230831
CGTGTCT450.095.230831
GTCTATA201.7310484E-594.990764
CGTCTAT201.7310484E-594.990763
CGTCTAG57350.094.990763
GCGGCTA201.7310484E-594.990762
CGGCTAG201.7310484E-594.990763
GATAGTC201.7310484E-594.990769
CTAGAGA156.5894064E-494.990756
GATACTT156.5894064E-494.990759
TGATACT156.5894064E-494.990758
TCTAGTT156.5894064E-494.990755
CCGTCTA156.5894064E-494.990752
GTTACTC156.5894064E-494.990759
CTAGTTA156.5894064E-494.990756
CTTGATA156.5894064E-494.990756
GCCTAGA156.5894064E-494.990754
GCGTCTA57500.094.990752
Produced by FastQC (version 0.11.5)