FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17270
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGTGGCACGATGAAACC1320.7643312101910829No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGAGCTTGAAAAGGAG1030.5964099594672843No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGTGACAAAAACATAA590.34163288940359005No Hit
CGTCGTACGATAGCACTAGTATCGCCGGGGAAGAAGTGGACGTTTGAGTG480.27793862188766644No Hit
CGTCGTACGATAGCACTAGTATGGGGGACGAGAGCGAGACTTCATCTCCA380.22003474232773595No Hit
CGTCGTACGATAGCACTAGTATCGGCTCTAGATTAAAGATTTAGATGCAG370.2142443543717429No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGTTTATTTATTTAGG320.18529241459177764No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAGCTTCGTGCTGGAGAAAA320.18529241459177764No Hit
CGTCGTACGATAGCACTAGTATCGGCGCTGTCTTCGAAATGGATGGCACT270.1563404748118124No Hit
CGTCGTACGATAGCACTAGTATCGGCCCCATGATCCAAACACCTCCCACC230.13317892298784018No Hit
CGTCGTACGATAGCACTAGTATCGCCGCCCCTGGAAGGACAAATCTTCTG230.13317892298784018No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGCTAGGACAAGTGAT230.13317892298784018No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGATAGGGTGAAAAATG220.12738853503184713No Hit
CGTCGTACGATAGCACTAGTATCGGCGCCCCTGGAAGGACAAATCTTCTG220.12738853503184713No Hit
CGTCGTACGATAGCACTAGTATCCGCGTGATCAGCAAAAGCAGGTGGCAC210.12159814707585408No Hit
CGTCGTACGATAGCACTAGTATCAGCAAAAGCAGGTGGCACGATGAAACC200.11580775911986102No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAGTAGAAAAGAAAAGGGAG200.11580775911986102No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAATACCAATCAGCAAAAGG200.11580775911986102No Hit
CGTCGTACGATAGCACTAGTATCGGCACTCCCATTCGCTTCTGGTAGGCC190.11001737116386798No Hit
CGTCGTACGATAGCACTAGTATCGCCCCCATGATCCAAACACCTCCCACC190.11001737116386798No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGAAATGAAGCAGGGGGCTGCGC190.11001737116386798No Hit
CGTCGTACGATAGCACTAGTATGGGGGGCAAAAACATAATGGACTCCAAC190.11001737116386798No Hit
CGTCGTACGATAGCACTAGTATGGGGGGCGAGAGCGAGACTTCATCTCCA190.11001737116386798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA16550.095.259441
GATAGCG156.508588E-494.994219
CGATAGC16850.094.7123268
ACGATAG16800.094.711497
TACGATA16700.094.709796
GATAGCA16650.094.708949
GTACGAT16600.094.708085
GTCGTAC16550.094.7072142
CGTACGA16550.094.7072144
TCGTACG16500.094.7063453
GTATGCG1100.047.49710518-19
GTATGAG550.047.49710518-19
TATGCGG850.047.49710520-21
ATAGGGG301.4509078E-647.49710520-21
ATAGGCG452.1282176E-1047.49710520-21
TATCGCA550.047.49710520-21
ATGGCGG301.4509078E-647.49710520-21
GTATCCG650.047.49710518-19
GTATCAG301.4509078E-647.49710518-19
GTATAGC452.1282176E-1047.49710518-19
Produced by FastQC (version 0.11.5)