FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13671
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGACAGCGAGTCAGCGTAGCCCCCCAAGATCGAAGGTTCCAGGTTCC630.4608294930875576No Hit
CGACGACAGCGAGTCAGCGTAGGGGGGCTATATGAAGCAATCGAGGAGTG370.27064589276570844No Hit
CGACGACAGCGAGTCAGCGTAGCGCCCCAAGATCGAAGGTTCCAGGTTCC240.17555409260478386No Hit
CGACGACAGCGAGTCAGCGTAGCACCACATTCCCTTATACTGGAGATCCT220.16092458488771855No Hit
CGACGACAGCGAGTCAGCGTAGAGTCCCGTTTTCGTTTCATTACCAACAC220.16092458488771855No Hit
CGACGACAGCGAGTCAGCGTAGGCCCCCAAGATCGAAGGTTCCAGGTTCC200.1462950771706532No Hit
CGACGACAGCGAGTCAGCGTAGACCCCCAAGATCGAAGGTTCCAGGTTCC200.1462950771706532No Hit
CGACGACAGCGAGTCAGCGTAGGGAACGGGAACAGGATACACCATGGACA190.13898032331212054No Hit
CGACGACAGCGAGTCAGCGTAGGGGGGGGGAAGGCTCTATTGGGAAAGTG190.13898032331212054No Hit
CGACGACAGCGAGTCAGCGTAGAAACAAGGTCGTTTTTAAACAATTCGAC180.13166556945358787No Hit
CGACGACAGCGAGTCAGCGTAGGGCAAGGGAACGTAGTGTTGGTAATGAA180.13166556945358787No Hit
CGACGACAGCGAGTCAGCGTAGAGGGGGCCTGACGGGATGATAGAAAGAA160.11703606173652256No Hit
CGACGACAGCGAGTCAGCGTAGAGACAAGGTCGTTTTTAAACAATTCGAC160.11703606173652256No Hit
CGACGACAGCGAGTCAGCGTAGCACCCCAAGATCGAAGGTTCCAGGTTCC160.11703606173652256No Hit
CGACGACAGCGAGTCAGCGTAGATGCCATAAGCACCACATTCCCTTATAC150.10972130787798992No Hit
CGACGACAGCGAGTCAGCGTAGGGAACAGGAACAGGATACACCATGGACA150.10972130787798992No Hit
CGACGACAGCGAGTCAGCGTAGAGCAGCATATCCTTTTGTGGTGTAAACA140.10240655401945725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAGT13500.094.648158
CAGCGAG13450.094.646857
GCGAGTC13400.094.645529
GACGACA13250.094.641522
CGACGAC13150.094.638781
ACGACAG13400.094.2910463
CGACAGC13300.094.2857064
ACAGCGA13650.092.912086
GACAGCG13600.092.555155
CGTAGAG1350.047.50000418-19
GTAGATG252.7545679E-547.518-19
TAGCGGC501.0913936E-1147.520-21
GTAGAGG452.0918378E-1047.518-19
GTAGAGC501.0913936E-1147.518-19
CGTAGGT252.7545679E-547.518-19
CGTAGGG3350.047.518-19
CGTAGCT252.7545679E-547.518-19
CGTAGCG2150.047.518-19
AGGGAGG252.7545679E-547.520-21
TTCCATG252.7545679E-547.584-85
Produced by FastQC (version 0.11.5)