FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301754
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT131694.364150930890726No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT110393.658277935006661No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTAGATATT69412.300218058418447No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGTGACAAA59711.9787641588843892No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGTAGATATTTAAAGATGAGT34571.145635186277564No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTATTTT17990.5961809951152263No Hit
CGTCTATCATACGACTGTCTACAAGATCCCAATGATATTTGCTGCAATGA17700.5865705177064761No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGAGTTTAAA16660.5521053573440616No Hit
CGTCTATCATACGACTGTCTACATTCCCATTTAGGGCATTTTGGATAAAG16100.5335471940719925No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTCGTTTT13550.449041272029534No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGGTGACAAAAACATAATGGA13260.43943079462078377No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGAGTTTTT13200.43744241998449074No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGAGTTTAAAATGAATCCAAA12440.4122563412581109No Hit
CGTCTATCATACGACTGTCTACACCACAAAAGGATATGCTGCTCCCGCTA11560.38309351325914487No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGTAGATAA10190.3376922923971182No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGGAAAACA8630.28599455185349654No Hit
CGTCTATCATACGACTGTCTACAACACCATGTCAAGCTTTCAGGTAGACT7640.25318637035465974No Hit
CGTCTATCATACGACTGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGT7450.24688985067306482No Hit
CGTCTATCATACGACTGTCTACGCGGGATCAGCAAAAGCAGGTAGATATT7290.24158751830961644No Hit
CGTCTATCATACGACTGTCTACATGCGAACAGAGGTTATAAGAATGATGG6860.22733750008284895No Hit
CGTCTATCATACGACTGTCTACGGGGGCGAAACGTACGTTCTTTCTATCA6840.2266747085374179No Hit
CGTCTATCATACGACTGTCTACAACCGAGGTCGAAACGTACGTTCTTTCT6810.22568052121927132No Hit
CGTCTATCATACGACTGTCTACCAGTACCAATGAACTGGCGACAGTTGAA6560.21739562690138323No Hit
CGTCTATCATACGACTGTCTACCAAGATCCCAATGATATTTGCTGCAATG6260.20745375371991753No Hit
CGTCTATCATACGACTGTCTACCGAGGGCGAAACGTACGTTCTTTCTATC6180.2048025875381934No Hit
CGTCTATCATACGACTGTCTACCGGGGGCGAAACGTACGTTCTTTCTATC6130.20314560867461576No Hit
CGTCTATCATACGACTGTCTACGCGGGATCAGTAGAAACAAGGTAGTTTT5720.1895583819932793No Hit
CGTCTATCATACGACTGTCTACCGGGGCGAAACGTACGTTCTTTCTATCA5560.18425604962983091No Hit
CGTCTATCATACGACTGTCTACATGCAATGACGAGAGGATCACTTGAATC5460.1809420919026757No Hit
CGTCTATCATACGACTGTCTACCGAGGTCGAAACGTACGTTCTTTCTATC5220.17298859335750313No Hit
CGTCTATCATACGACTGTCTACCCGACCAGTGAGTACCCTTCCCTTTCAA5160.17100021872121No Hit
CGTCTATCATACGACTGTCTACCAGGGGCGAATGGGAGTGCAGATGCAGC5160.17100021872121No Hit
CGTCTATCATACGACTGTCTACGGGGGGAAAATGGACGAAGGACAAGGGT5110.1693432398576324No Hit
CGTCTATCATACGACTGTCTACCGAAGAAATAAGACCCTTCATTACTCAT4810.15940136667616667No Hit
CGTCTATCATACGACTGTCTACCAGAGCAGAAATGAAAAGTGGCGAGAGC4520.14979088926741652No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGGGAAAACAAAAGCAACAAA4470.1481339104038389No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGGGTTTT4350.14415716113125263No Hit
CGTCTATCATACGACTGTCTACGGGGGGCCAAGGAGGTGTCACTAAGCTA4300.142500182267675No Hit
CGTCTATCATACGACTGTCTACCGAGGCCGAAACGTACGTTCTTTCTATC4240.14051180763138185No Hit
CGTCTATCATACGACTGTCTACCCCCCTTCAATGAAGCCGGCAATGGCCC4140.13719784990422662No Hit
CGTCTATCATACGACTGTCTACCCGAACAACATGGATAGAGCAGTTAAAC4140.13719784990422662No Hit
CGTCTATCATACGACTGTCTACTAACCGAGGTCGAAACGTACGTTCTTTC4110.13620366258608005No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTCAAATAT4100.13587226681336453No Hit
CGTCTATCATACGACTGTCTACGCGTGCTCAGCAAAAGCAGGTAGATATT4090.13554087104064902No Hit
CGTCTATCATACGACTGTCTACAAGGTAGTTTTTTACTCTAGCTCTATGT4070.13487807949521796No Hit
CGTCTATCATACGACTGTCTACCATGTTGTTCGGGTCCCCATTCCCATTT3950.13090133022263167No Hit
CGTCTATCATACGACTGTCTACGCGCGATCAGCAAAAGCAGGTAGATATT3950.13090133022263167No Hit
CGTCTATCATACGACTGTCTACGAGAGGATCACTTGAATCGCTGCATCTG3880.12858155981362301No Hit
CGTCTATCATACGACTGTCTACAACCGATTAGAGACCTTGATACTACTAA3850.12758737249547644No Hit
CGTCTATCATACGACTGTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATA3820.12659318517732987No Hit
CGTCTATCATACGACTGTCTACCCGAGAGCAATTGGGACAGAAATTTGAG3780.12526760208646778No Hit
CGTCTATCATACGACTGTCTACCGAGGGGGAAACGTACGTTCTTTCTATC3680.12195364435931255No Hit
CGTCTATCATACGACTGTCTACAAGTGGCACACACTAGACCAAAAGCAGC3660.1212908528138815No Hit
CGTCTATCATACGACTGTCTACAGACACTGTAGACACAGTACTAGAAAAG3630.12029666549573494No Hit
CGTCTATCATACGACTGTCTACGAGGGCGAAACGTACGTTCTTTCTATCA3580.11863968663215732No Hit
CGTCTATCATACGACTGTCTACCAGAAGCGAATGGGAGTGCAGATGCAGC3530.1169827077685797No Hit
CGTCTATCATACGACTGTCTACCGGGAGCGAATGGGAGTGCAGATGCAGC3460.11466293735957105No Hit
CGTCTATCATACGACTGTCTACCGGGGACGAAACGTACGTTCTTTCTATC3340.11068618808698476No Hit
CGTCTATCATACGACTGTCTACAAAAGCAACAAAAATGAAGGCAATACTA3290.10902920922340714No Hit
CGTCTATCATACGACTGTCTACATCCGATCACAATTGGAAAATGTCCAAA3240.10737223035982953No Hit
CGTCTATCATACGACTGTCTACCAGGTGCAAGATCCCAATGATATTTGCT3220.10670943881439848No Hit
CGTCTATCATACGACTGTCTACGCGGGATCAGTAGAAACAAGGGTGTTTT3190.10571525149625192No Hit
CGTCTATCATACGACTGTCTACGCGTGCTCAGTAGAAACAAGGTAGTTTT3190.10571525149625192No Hit
CGTCTATCATACGACTGTCTACGAGGGGGGAAAATGGACGAAGGACAAGG3120.10339548108724325No Hit
CGTCTATCATACGACTGTCTACCCGAGGCCAAGGGTGTTGCCTCTTCCTT3060.10140710645095011No Hit
CGTCTATCATACGACTGTCTACGAGGCCGAAACGTACGTTCTTTCTATCA3040.10074431490551908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT950.095.218571
CGTCGAT156.553009E-495.218571
CGTTTAT353.237801E-1095.218571
TAATACG800.094.997697
GTCGATC156.613798E-494.997682
ATGATAC550.094.997686
ATATGAC750.094.997689
ATACGAC295900.093.970339
CATACGA295200.093.9678968
GTCTATC297550.093.880252
TATCATA297400.093.8637165
TCATACG296350.093.8596957
ATCATAC296000.093.826266
CGTCTAT298700.093.815951
TCGTACG1000.090.2477957
TATAATA950.089.99785
TCTATCG950.089.99783
TCTATAA950.089.99783
TTATACG750.088.6645057
CATAGGA700.088.212138
Produced by FastQC (version 0.11.5)