FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_258.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_258.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68368
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGACTCTGTCT4360.6377252515796864No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGAGCAAGAGAG3950.57775567516967No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGAGTTGAACT3740.5470395506669787No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGAATCTCTGC2310.3378773695296045No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGGTGTTTTC2070.3027732272408144No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGTTCAAAGGTC1880.2749824479288556No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGTGAAAGATGT1820.2662064123566581No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGCTAGGACAA1780.2603557219751931No Hit
CGTAGAGTATCTGAGTATACGCCCAACAAATAACTGATAATTCTCAACAG1570.22963959747250173No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGAGCTTGAAA1470.21501287151883924No Hit
CGTAGAGTATCTGAGTATACGCGGGGGGGGAAAGGAAATGTAATGCATTT1420.20769950854200797No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGTGACAAAAA1220.17844605663468288No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGTTGATGTGCT1150.16820734846711913No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGAAGCCAACA1130.16528200327638662No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGAAGCTTCA1080.15796864029955535No Hit
CGTAGAGTATCTGAGTATACGCCGGCGCCAAACCTTATGTGAAATTGTAA1020.14919260472735782No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGTGTTTTTT1010.1477299321319916No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGTGATCTTCC880.12871518839223028No Hit
CGTAGAGTATCTGAGTATACGCGGGGGGGCAAGTGTGAGTGAATGTGTGT870.12725251579686403No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGATGATCTCCA850.12432717060613153No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGTCGTTTTTA830.12140182541539903No Hit
CGTAGAGTATCTGAGTATACGCCAGAGCAGAAATGAAAAGTGGCGAGAGC810.11847648022466652No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGTGGTGGTCA760.11116311724783524No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGACCCTGGCAA730.1067750994617365No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGAAGATTCTTA690.10092440908027148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG156.5721315E-495.068821
AGGGTAT156.5912976E-494.999264
AGTATCA156.5912976E-494.999266
GGTATCT156.5912976E-494.999266
TAGGGTA156.5912976E-494.999263
TATAGTA156.5912976E-494.999263
AGTAGCT156.5912976E-494.999266
ATCTGCG156.5912976E-494.999269
GTAGAGT66000.094.711392
GTATCTG65750.094.71037
AGTATCT65700.094.637786
TATCTGA65500.094.636688
TAGAGTA65900.094.5667953
ATCTGAG66900.094.502269
GAGTATC65900.094.422645
AGAGTAT66150.094.353014
CGTAGAG67750.094.0163961
GAGCATC850.083.822885
CATCTGA800.083.124378
GTAGAGC950.079.999382
Produced by FastQC (version 0.11.5)