FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_257.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_257.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20403
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATAGTCGTACGTATACATCCGGCCATCTGTTCCATAGCCTTTGCCGT1260.6175562417291575No Hit
CGCATAGTCGTACGTATACATCGGGGGGGGAACAGTGACCACAGAAGCTG1060.5195314414546881No Hit
CGCATAGTCGTACGTATACATCCGGCGCCCAATTAGCACATTAGCTTTCT900.4411116012351125No Hit
CGCATAGTCGTACGTATACATCCGGCGCAAAATGCCATAAGCACCACATT810.3970004411116012No Hit
CGCATAGTCGTACGTATACATCCGGAGCAGAAATGAAAAGTGGCGAGAGC660.32348184090574916No Hit
CGCATAGTCGTACGTATACATCGGGGGGGCAAGAGAAGAACCCCGCACTC640.31367936087830217No Hit
CGCATAGTCGTACGTATACATCGGGGGGGGAAGAGAAGAACCCCGCACTC600.2940744008234083No Hit
CGCATAGTCGTACGTATACATCAGGAGCTAATGGTAGTGCCACCATCCAG360.17644464049404499No Hit
CGCATAGTCGTACGTATACATCCGGCCCTAGACACCATGGCCTCCACCAT320.15683968043915109No Hit
CGCATAGTCGTACGTATACATCGGGGGAAGAATATCAACAGGAACAGCAG310.15193844042542762No Hit
CGCATAGTCGTACGTATACATCCGGCCCCTTGCCCAATTAGCACATTAGC300.14703720041170415No Hit
CGCATAGTCGTACGTATACATCCAGAGCAGAAATGAAAAGTGGCGAGAGC300.14703720041170415No Hit
CGCATAGTCGTACGTATACATCAGGCGCAAAATGCCATAAGCACCACATT300.14703720041170415No Hit
CGCATAGTCGTACGTATACATCAGGCGCCCAATTAGCACATTAGCTTTCT300.14703720041170415No Hit
CGCATAGTCGTACGTATACATCGGGGGGCAAGAGAAGAACCCCGCACTCA300.14703720041170415No Hit
CGCATAGTCGTACGTATACATCCAGCGCAAAATGCCATAAGCACCACATT270.13233348037053375No Hit
CGCATAGTCGTACGTATACATCAGTAGAAACAAGGTCGTTTTTAAACAAT260.12743224035681028No Hit
CGCATAGTCGTACGTATACATCAGGGGGGGAACAGTGACCACAGAAGCTG260.12743224035681028No Hit
CGCATAGTCGTACGTATACATCCAGCCATCTGTTCCATAGCCTTTGCCGT240.11762976032936333No Hit
CGCATAGTCGTACGTATACATCAGGCACTCCTTCCGTAGAAGGCCCTCTT240.11762976032936333No Hit
CGCATAGTCGTACGTATACATCGGGAGGGGAACAGTGACCACAGAAGCTG230.11272852031563986No Hit
CGCATAGTCGTACGTATACATCAGGCCCCCTCAAAGCCGAGATCGCGCAG230.11272852031563986No Hit
CGCATAGTCGTACGTATACATCCGGCGCCCAAGATCGAAGGTTCCAGGTT230.11272852031563986No Hit
CGCATAGTCGTACGTATACATCCGGGGGGGAACAGTGACCACAGAAGCTG220.10782728030191639No Hit
CGCATAGTCGTACGTATACATCCGCCGCCCAATTAGCACATTAGCTTTCT220.10782728030191639No Hit
CGCATAGTCGTACGTATACATCCGGCCAGACTCAAAGTCGACCCTGGCAT220.10782728030191639No Hit
CGCATAGTCGTACGTATACATCCCGCACTCAGAATGAAGTGGATGATGGC220.10782728030191639No Hit
CGCATAGTCGTACGTATACATCGGGGGGGGAAAGGAAATGTAATGCATTT220.10782728030191639No Hit
CGCATAGTCGTACGTATACATCCTGAGCAGAAATGAAAAGTGGCGAGAGC210.10292604028819291No Hit
CGCATAGTCGTACGTATACATCGGGGAGGCAAGAGAAGAACCCCGCACTC210.10292604028819291No Hit
CGCATAGTCGTACGTATACATCCCGCCAGGCCCCCTCAAAGCCGAGATCG210.10292604028819291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA201.7031289E-594.997559
GCATAGG156.524992E-494.997552
CGCATAG19900.094.752411
GCATAGT19850.094.2796862
ATAGTCG19750.094.2760544
TAGTCGT19700.094.274215
AGTCGTA19650.094.272376
GTCGTAC19600.094.270527
TCGTACG19600.094.270528
CATAGTC19950.093.569013
CGTACGT19700.093.309779
GCGGGGC205.327613E-447.49877524-25
ACAGCAT452.1464075E-1047.49877594-95
TGTGAAC452.1464075E-1047.49877574-75
GGTTGTC404.0545274E-947.49877554-55
TAGTGTG452.1464075E-1047.49877560-61
CAGCATC404.0545274E-947.49877594-95
TCGACGG205.327613E-447.49877520-21
TGACCAC404.0545274E-947.49877536-37
GGCGTGA205.327613E-447.49877524-25
Produced by FastQC (version 0.11.5)