FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60792
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTCGTTT5160.8487958941966048No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGCCAAATA4350.7155546782471378No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTCAAATA3710.6102776681142256No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGGTGACAA3490.574088695881037No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTAGTTT3420.5625740228977497No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTAGATAT2860.4704566390314515No Hit
CGATACAGCTACTACTACTCTACGGAGCAGAAATGAAAAGTGGCGAGAGC2470.40630346098170816No Hit
CGATACAGCTACTACTACTCTACTAACCATTAATCTTGCGTAGCAGAGGC2350.38656402158178704No Hit
CGATACAGCTACTACTACTCTACAGAGCAGAAATGAAAAGTGGCGAGAGC2330.38327411501513353No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCCGTTT2200.36188972233188577No Hit
CGATACAGCTACTACTACTCTACGGCAAAGGCTATGGAACAGATGGCTGG2060.3388603763653112No Hit
CGATACAGCTACTACTACTCTAACGCGAAGTAGGTACAGATGCAATTGTC1670.27470719831556784No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGGTGTTT1670.27470719831556784No Hit
CGATACAGCTACTACTACTCTAGGGGGGCTATATGAAGCAATCGAGGAGT1550.2549677589156468No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGCAGATAT1430.23522831951572576No Hit
CGATACAGCTACTACTACTCTAATTCGCTTCTGGTAGGCCTGCAAATTTT1420.23358336623239903No Hit
CGATACAGCTACTACTACTCTACTGAGCAGAAATGAAAAGTGGCGAGAGC1400.23029345966574546No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGCGACAAA1290.2121989735491512No Hit
CGATACAGCTACTACTACTCTAAGCCGAGATCGCGCAGAGACTGGAAAGT1170.19245953414923014No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTGACAAA1170.19245953414923014No Hit
CGATACAGCTACTACTACTCTAAGACCACTGTGGACCATATGGCCATAAT1140.1875246742992499No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCAGTTT1090.17929990788261613No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCGATCT990.1628503750493486No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTACTGAT980.16120542176602184No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCATTTT950.15627056191604158No Hit
CGATACAGCTACTACTACTCTACCCCGCACTCAGAATGAAGTGGATGATG950.15627056191604158No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTACTTT920.15133570206606134No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTTGATGT910.14969074878273458No Hit
CGATACAGCTACTACTACTCTACGGCCCCTTGCCCAATTAGCACATTAGC910.14969074878273458No Hit
CGATACAGCTACTACTACTCTACCTCCAGAGCAGAAATGAAAAGTGGCGA900.1480457954994078No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTTGACAA870.14311093564942756No Hit
CGATACAGCTACTACTACTCTAAGCAGAAATGAAAAGTGGCGAGAGCAAT840.1381760757994473No Hit
CGATACAGCTACTACTACTCTATGGGGCCAAGGAGGTGTCACTAAGCTAT830.13653112251612054No Hit
CGATACAGCTACTACTACTCTAAGGCCACTGTGGACCATATGGCCATAAT820.13488616923279378No Hit
CGATACAGCTACTACTACTCTATTGGGCAAGGGGACGTAGTGTTGGTAAT800.13159626266614027No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCGTTTT690.11350177654954599No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTGATCT650.10692196341623898No Hit
CGATACAGCTACTACTACTCTAGGGGGGGGAAGCTTCGTGCTGGAGAAAA630.10363205684958546No Hit
CGATACAGCTACTACTACTCTAGCGGGAGTTTTGTTCAGCATCCAGAACT630.10363205684958546No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGCGTTTAA620.10198710356625872No Hit
CGATACAGCTACTACTACTCTACGTCCCCTTGCCCAATTAGCACATTAGC610.10034215028293196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA156.460349E-495.466511
CGATAGA254.436224E-795.466511
GCTACTG156.5888924E-494.9950568
CGATACA59300.094.9030461
ACAGCTA59750.094.359115
CAGCTAC59750.094.359116
AGCTACT60200.094.2849667
TACAGCT59900.094.281414
GATACAG59650.094.278422
ATACAGC60100.094.1257253
GCTACTA60250.093.812558
GAGCTAC301.352184E-679.162556
GCAACTA255.2365533E-575.996058
GCTGCTA255.2365533E-575.996058
CGAGACA200.00202482771.599881
CGATGCA200.00202482771.599881
GTACAGC200.002065030271.24633
GCAGCTA200.002065030271.24635
TGCAGCT200.002065030271.24634
AGCAACT301.2917035E-463.330047
Produced by FastQC (version 0.11.5)