FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12212
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTATACTCGCACGAGCGTCCAGAGCAGAAATGAAAAGTGGCGAGAGC470.3848673435964625No Hit
CGCGTATACTCGCACGAGCGTCAGGAGCTAATGGTAGTGCCACCATCCAG440.3603013429413692No Hit
CGCGTATACTCGCACGAGCGTCCAACACCTTTTTGGATCTAAAAAGTACT420.3439240091713069No Hit
CGCGTATACTCGCACGAGCGTCCAGGGGGGAAGAATATCAACAGGAACAG340.27841467409105797No Hit
CGCGTATACTCGCACGAGCGTCCGTAGAAGGCCCTCTTTTCAAACCGTAT340.27841467409105797No Hit
CGCGTATACTCGCACGAGCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAG290.2374713396659024No Hit
CGCGTATACTCGCACGAGCGTCCAAGAGGGAAGAATATCAACAGGAACAG280.22928267278087125No Hit
CGCGTATACTCGCACGAGCGTCCAGGAGGGAAGAATATCAACAGGAACAG270.22109400589584016No Hit
CGCGTATACTCGCACGAGCGTCCGGAGCTAATGGTAGTGCCACCATCCAG260.21290533901080905No Hit
CGCGTATACTCGCACGAGCGTCCGGGGGGGAAGAATATCAACAGGAACAG250.20471667212577793No Hit
CGCGTATACTCGCACGAGCGTCATTGCAGCAAATATCATTGGGATCTTGC250.20471667212577793No Hit
CGCGTATACTCGCACGAGCGTCCGGGAGGGAAGAATATCAACAGGAACAG240.1965280052407468No Hit
CGCGTATACTCGCACGAGCGTCGGGGGAAGAATATCAACAGGAACAGCAG230.18833933835571567No Hit
CGCGTATACTCGCACGAGCGTCCGGGGAAGAATATCAACAGGAACAGCAG230.18833933835571567No Hit
CGCGTATACTCGCACGAGCGTCAGTAGAAACAAGGTCGTTTTTAAACAAT220.1801506714706846No Hit
CGCGTATACTCGCACGAGCGTCCGGAGCAGAAATGAAAAGTGGCGAGAGC190.15558467081559121No Hit
CGCGTATACTCGCACGAGCGTCGGGGCAGCAAATATCATTGGGATCTTGC190.15558467081559121No Hit
CGCGTATACTCGCACGAGCGTCAGTAGAAACAAGGTAGTTTTTTACTCTA190.15558467081559121No Hit
CGCGTATACTCGCACGAGCGTCAGGATATAGCGGGAGTTTTGTTCAGCAT180.1473960039305601No Hit
CGCGTATACTCGCACGAGCGTCGGGAGCTAATGGTAGTGCCACCATCCAG180.1473960039305601No Hit
CGCGTATACTCGCACGAGCGTCGGGGGGCCAAGGAGGTGTCACTAAGCTA180.1473960039305601No Hit
CGCGTATACTCGCACGAGCGTCCATGAGGGAAGAATATCAACAGGAACAG170.13920733704552898No Hit
CGCGTATACTCGCACGAGCGTCCGGGACAGAAATTTGAGGAAATAAGGTG170.13920733704552898No Hit
CGCGTATACTCGCACGAGCGTCATCAGCAAAAGCAGGTAGATATTTAAAG160.13101867016049787No Hit
CGCGTATACTCGCACGAGCGTCCTGAGAGGATTTCTCATTTTAGGCAAAG150.12283000327546675No Hit
CGCGTATACTCGCACGAGCGTCAGGAAGGCAAGAGAAGAACCCCGCACTC150.12283000327546675No Hit
CGCGTATACTCGCACGAGCGTCGAGGGAAGAATATCAACAGGAACAGCAG150.12283000327546675No Hit
CGCGTATACTCGCACGAGCGTCATCAGCAAAAGCAGGTCAAATATATTCA150.12283000327546675No Hit
CGCGTATACTCGCACGAGCGTCATGAACTGAGCAATCTTGCAAAAGGAGA150.12283000327546675No Hit
CGCGTATACTCGCACGAGCGTCCGACACCTTTTTGGATCTAAAAAGTACT150.12283000327546675No Hit
CGCGTATACTCGCACGAGCGTCGGGACAGAAATTTGAGGAAATAAGGTGG140.11464133639043562No Hit
CGCGTATACTCGCACGAGCGTCGGGCCCCCTCAAAGCCGAGATCGCGCAG130.10645266950540452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT11850.096.170131
ACTCGCA11900.094.987718
CGTATAC12050.094.987713
GCGTATA12050.094.987712
ATACTCG12050.094.987716
TACTCGC11900.094.987717
ACTCGGA156.4640585E-494.98778
CTCGGAC156.4640585E-494.98779
TACTCGG156.4640585E-494.98777
CTCGCAC11950.094.98779
TATACTC12100.094.595195
GTATACT12250.094.2122964
CGTCAGA357.509516E-847.49385518-19
TGTTGCT205.28791E-447.49385582-83
CGTCACG252.747946E-547.49385518-19
CGTCAAA252.747946E-547.49385518-19
CTGAGCT205.28791E-447.49385576-77
GTCATCA252.747946E-547.49385520-21
TAGTGCC205.28791E-447.49385534-35
CGTTAAT205.28791E-447.49385566-67
Produced by FastQC (version 0.11.5)