FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249581
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTATACGTACGTCTCAGTCAGGCCAAACCTAACAAGGCTAGTCTTAC7560.3029076732603844No Hit
CGAGTATACGTACGTCTCAGTCACACAGTGATCTCGTATGCCGTCTTCTG5960.23880022918411256RNA PCR Primer, Index 5 (97% over 34bp)
CGAGTATACGTACGTCTCAGTCCAGGGGGGAAGAATATCAACAGGAACAG4210.16868271222569026No Hit
CGAGTATACGTACGTCTCAGTCAGGCCCCCTCAAAGCCGAGATCGCGCAG4200.16828204070021355No Hit
CGAGTATACGTACGTCTCAGTCACCACCAAGGTCATGGTGCTAAGCCATT4110.16467599697092328No Hit
CGAGTATACGTACGTCTCAGTCCTACGCGTGATCAGCAAAAGCAGGTTCA3730.1494504790028087No Hit
CGAGTATACGTACGTCTCAGTCAAAAGCAGGTAGATATTTAAAGATGAGT3690.14784779290090191No Hit
CGAGTATACGTACGTCTCAGTCCAACACCTTTTTGGATCTAAAAAGTACT3590.1438410776461349No Hit
CGAGTATACGTACGTCTCAGTCAGGCGCCCTCAAAGCCGAGATCGCGCAG3520.14103637696779803No Hit
CGAGTATACGTACGTCTCAGTCCAGCGAGACACTTAGAATGACAATTGCA3400.13622831866207766No Hit
CGAGTATACGTACGTCTCAGTCCAGGAGGGAAGAATATCAACAGGAACAG3250.13021824577992716No Hit
CGAGTATACGTACGTCTCAGTCCACGCGTGATCAGCAAAAGCAGGTTCAA3210.12861555967802035No Hit
CGAGTATACGTACGTCTCAGTCCGGGGGGGAAGAATATCAACAGGAACAG3130.12541018747420676No Hit
CGAGTATACGTACGTCTCAGTCTACGCGTGATCAGCAAAAGCAGGTTCAA3060.12260548679586987No Hit
CGAGTATACGTACGTCTCAGTCCGGGGAAGAATATCAACAGGAACAGCAG2850.1141913847608592No Hit
CGAGTATACGTACGTCTCAGTCCCGCACTCGCGAGATACTCACTAAGACC2780.11138668408252231No Hit
CGAGTATACGTACGTCTCAGTCACGCGTGATCAGCAAAAGCAGGTTCAAA2610.10457526814941842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT301.2290911E-894.988599
CGTAGGT301.2290911E-894.988599
ACGTGCG301.2290911E-894.988598
CGTAAGT301.2290911E-894.988599
TATACGG650.094.988595
GTATCCG201.7421837E-594.988574
TATCCGT201.7421837E-594.988575
CGAGTAT247000.093.015441
GAGTATA249150.092.548582
TACGTAC248750.092.525567
GTATACG248200.092.46274
ATACGTA249250.092.4161766
TATACGT247750.092.400595
AGTATAC248600.092.333023
ACGTACG250850.091.580588
CGTACGT250700.091.559599
CGAGAAT600.087.4231951
CGTATGT600.087.072879
CGTACGG600.087.072879
TGCGTAC500.085.489727
Produced by FastQC (version 0.11.5)