FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_193.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_193.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455203
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGTATAGACGTGATACTGTGTCCATGGACACAGTAAACAGAACACA38190.8389663512762437No Hit
CGTATGTATAGACGTGATACTGTGTCCATGGAACAGGAACAGGATACACC31820.6990287849596774No Hit
CGTATGTATAGACGTGATACTGAAACGAGAAAGCTCTTATCTCTTGTTCT27810.6109362196646331No Hit
CGTATGTATAGACGTGATACTGGACCACAACTGCCTGTCTTATCATTAGG27560.6054441644716753No Hit
CGTATGTATAGACGTGATACTGAAGAGCTTCCCAGCACTGGGATGCTCTT24400.5360245868326878No Hit
CGTATGTATAGACGTGATACTGGCGGGGAGCGCCAGGATGCCACAGAAAT22960.5043903489212506No Hit
CGTATGTATAGACGTGATACTGAGGCCTATTCAACTGGTGCACTTGCCAG18720.4112450928486851No Hit
CGTATGTATAGACGTGATACTGAAAAGGGAAAGATAGTCAAATCAGTCGA15330.33677282443217643No Hit
CGTATGTATAGACGTGATACTGAAGATCTTGTATGAGGCCTGTCCATCAC13270.2915182896422036No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGTAGATATTTAAAGATGAGT12470.2739437130247384No Hit
CGTATGTATAGACGTGATACTGAGGAGCTAAGAGAGCAATTGAGCTCAGT11900.2614218271847945No Hit
CGTATGTATAGACGTGATACTGGGGGGGGGAAAATGGACGAAGGACAAGG11610.2550510431609634No Hit
CGTATGTATAGACGTGATACTGGTGGGGAGCGCCAGGATGCCACAGAAAT10890.2392339242052447No Hit
CGTATGTATAGACGTGATACTGGGGGGGAGCGCCAGGATGCCACAGAAAT10820.2376961487512165No Hit
CGTATGTATAGACGTGATACTGTTTTCTTAGGGTCCTTCCCAGCACTGGG9970.21902316109515974No Hit
CGTATGTATAGACGTGATACTGTTACATTCTTTTCTAGTACTGTGTCTAC9480.20825873291696234No Hit
CGTATGTATAGACGTGATACTGGAGGGGGGAAAATGGACGAAGGACAAGG9100.19991080902366634No Hit
CGTATGTATAGACGTGATACTGAGAAACAAGGCATTTTTTCATGAAGGAC9060.19903208019279311No Hit
CGTATGTATAGACGTGATACTGAAACAAGGCATTTTTTCATGAAGGACAA8960.19683525811560995No Hit
CGTATGTATAGACGTGATACTGCAAGCCCATACACACAAGCAGGCAGGCA8870.19485811824614513No Hit
CGTATGTATAGACGTGATACTGAAGCGAATGGGAGTGCAGATGCAGCGAT8740.19200224954580702No Hit
CGTATGTATAGACGTGATACTGAACAAAACTCCCGCTATATCCTGACCAC8180.1797000459135814No Hit
CGTATGTATAGACGTGATACTGATTCCCAAGTTGAATTGAGTGGCTAATC7910.1737686263051869No Hit
CGTATGTATAGACGTGATACTGATGCAATTGTCGTTCTAAGTGTCTCGCT7820.1717914864357221No Hit
CGTATGTATAGACGTGATACTGAATAGCATAACAATAGAGAGGATGGTGC7620.1673978422813558No Hit
CGTATGTATAGACGTGATACTGCAGAGACTGGAAAGTGTCTTTGCAGGAA7510.16498133799645434No Hit
CGTATGTATAGACGTGATACTGGGAGGCTGGTGTTTATAGCACCCTTGGG7400.16256483371155286No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGGTGACAAAAACATAATGGA7260.15948928280349647No Hit
CGTATGTATAGACGTGATACTGATTGCCCCCAGGGAGACTACCAGTACCA7130.15663341410315837No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGGTAGATATTTAAAGATGAGT7130.15663341410315837No Hit
CGTATGTATAGACGTGATACTGCGCAGAGACTGGAAAGTGTCTTTGCAGG7100.15597436748000343No Hit
CGTATGTATAGACGTGATACTGATCCAAAATGGAAGACTTTGTGCGACAA6940.15245945215651038No Hit
CGTATGTATAGACGTGATACTGTTTACACCACAAAAGGATATGCTGCTCC6790.14916421904073568No Hit
CGTATGTATAGACGTGATACTGGAAAAGGGAAAGATAGTCAAATCAGTCG6650.14608866813267926No Hit
CGTATGTATAGACGTGATACTGAGGCTCTCATGGAATGGCTAAAGACAAG6420.14103597735515805No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGCGTTTAAAATGAATCCAAA6160.13532423995448184No Hit
CGTATGTATAGACGTGATACTGGACGGATCATGATCTCAGAGAACTCTTC6100.13400614670817196No Hit
CGTATGTATAGACGTGATACTGAAGAGCACACAGAATGCCATTGACGAGA6040.13268805346186208No Hit
CGTATGTATAGACGTGATACTGAAAAGGGGAAGATAGTCAAATCAGTCGA6010.1320290068387071No Hit
CGTATGTATAGACGTGATACTGATCAGCAAAAGCAGGTAGATATTTAAAG5790.12719599826890418No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGAAAGCACCATCCTCTCTATT5650.1241204473608478No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGAGTTTAAAATGAATCCAAA5630.12368108294541116No Hit
CGTATGTATAGACGTGATACTGAAACAAGGTAGTTTTTTACTCTAGCTCT5580.1225826719068196No Hit
CGTATGTATAGACGTGATACTGGCGGGGGGCGCCAGGATGCCACAGAAAT5570.12236298969910128No Hit
CGTATGTATAGACGTGATACTGTTGTGGTCCAGTATCGTCTAATGGAGCA5560.12214330749138295No Hit
CGTATGTATAGACGTGATACTGATCCCCTCAGAATGAGTGCTGACCGTGC5340.11731029892158006No Hit
CGTATGTATAGACGTGATACTGACCATCGTCAACATCCACAGCACTCTGC5330.11709061671386171No Hit
CGTATGTATAGACGTGATACTGGGGGGGGAAAATGGACGAAGGACAAGGG5270.11577252346755185No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGTCAAATATATTCAATATGG5180.11379538359808702No Hit
CGTATGTATAGACGTGATACTGATTAGCAACCTCCATGGCCTCTGCTGCC5120.11247729035177713No Hit
CGTATGTATAGACGTGATACTGAAATGGGAGGCTGGTGTTTATAGCACCC5090.11181824372862217No Hit
CGTATGTATAGACGTGATACTGAATAGCATAACAATAGAAAGCACCATCC5030.11050015048231229No Hit
CGTATGTATAGACGTGATACTGGAAATCTAGTGGTACCGAGATATGCATT4960.1089623750282841No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGTAAACCATTTGAATGGATG4930.10830332840512913No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGGGTGACAAAAACATAATGGA4890.10742459957425589No Hit
CGTATGTATAGACGTGATACTGAAGAAACAAGGCATTTTTTCATGAAGGA4850.10654587074338262No Hit
CGTATGTATAGACGTGATACTGAACACACCAATACTCAGAAAAGAACAGA4830.10610650632794599No Hit
CGTATGTATAGACGTGATACTGAGAAACAAGGTAGTTTTTTACTCTAGCT4830.10610650632794599No Hit
CGTATGTATAGACGTGATACTGAGCAGAAATGAAAAGTGGCGAGAGCAAT4750.10434904866619948No Hit
CGTATGTATAGACGTGATACTGAGGAGATTTCATCGATTATGAGGAGCTA4660.10237190879673465No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGGAGTTTAAAATGAATCCAAA4630.1017128621735797No Hit
CGTATGTATAGACGTGATACTGCAGCGATTCAAGTGATCCTCTCGTCATT4630.1017128621735797No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGGCGTTTAAAATGAATCCAAA4620.10149317996586138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTG450.094.995392
TACACGT201.7431137E-594.995399
CGTATAG254.6174864E-794.9953845
GTGTATA1000.094.9953844
TATACGT156.6166144E-494.9953849
GTATAGA447900.094.79396
TATAGAC446900.094.793457
TAGACGT446050.094.793079
ATAGACG448550.094.645948
CGTATGT449700.094.570111
GTATGTA450450.094.425992
TGTATAG450500.094.352245
ATGTATA451900.093.944324
TATGTAT459600.092.380743
TGGACGT1100.090.6774149
ATGTATG1100.090.6774144
CGTGTGT1050.090.631081
ATAGGCG900.089.7178658
TATAGGC900.089.7178657
TATGTAG800.089.058183
Produced by FastQC (version 0.11.5)