FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30831
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGACGTACAGCGCGTGTAAGTAGAAACAAGGTAGTTTTTTACTCTA1120.363270734001492No Hit
CGCGTGACGTACAGCGCGTGTACGCGTGATCAGTAGAAACAAGGGTGTTT830.2692095618046771No Hit
CGCGTGACGTACAGCGCGTGTAAGGCTTGCATAAATGTTATTTGTTCGAA680.22055723135804872No Hit
CGCGTGACGTACAGCGCGTGTAATGACCGATGGACCAAGTGATGGACAGG560.181635367000746No Hit
CGCGTGACGTACAGCGCGTGTAATCAGCAAAAGCAGGAGTTTAAAATGAA540.17514838960786222No Hit
CGCGTGACGTACAGCGCGTGTAATCAGCAAAAGCAGGCGTTTAAAATGAA540.17514838960786222No Hit
CGCGTGACGTACAGCGCGTGTACGCGTGATCAGTAGAAACAAGGTAGTTT510.16541792351853654No Hit
CGCGTGACGTACAGCGCGTGTAAAGCGAATCCAAACCAAAAGATAATAAC440.1427135026434433No Hit
CGCGTGACGTACAGCGCGTGTAATCAGCAAAAGCAGGGTGACAAAAACAT440.1427135026434433No Hit
CGCGTGACGTACAGCGCGTGTAAGCAAAAGCAGGCGTTTAAAATGAATCC430.1394700139470014No Hit
CGCGTGACGTACAGCGCGTGTAGGGTGACAAAAACATAATGGACTCCAAC420.1362265252505595No Hit
CGCGTGACGTACAGCGCGTGTAATCAGCAAAAGCAGGTAGATATTTAAAG410.1329830365541176No Hit
CGCGTGACGTACAGCGCGTGTATGCTAGAGTCCCGTTTTCGTTTCATTAC410.1329830365541176No Hit
CGCGTGACGTACAGCGCGTGTACAGCAAAAGCAGGCGTTTAAAATGAATC380.12325257046479193No Hit
CGCGTGACGTACAGCGCGTGTACAAAAACATAATGGACTCCAACACCATG380.12325257046479193No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGACAAAAACATAATGGACTCCAAC370.12000908176835004No Hit
CGCGTGACGTACAGCGCGTGTAAATGGCAACTCAGCACCGTCTGGCCAAG360.11676559307190815No Hit
CGCGTGACGTACAGCGCGTGTAAGCAGGGGAAAACAAAAGCAACAAAAAT360.11676559307190815No Hit
CGCGTGACGTACAGCGCGTGTACAGAGGGCAACCCACAGTGTCACTGTTT360.11676559307190815No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGGCAAAAACATAATGGACTCCAAC340.11027861567902436No Hit
CGCGTGACGTACAGCGCGTGTAAGCAAAAGCAGGAGTTTAAAATGAATCC340.11027861567902436No Hit
CGCGTGACGTACAGCGCGTGTACGCGTGATCAGTAGAAACAAGGCGTTTT330.10703512698258247No Hit
CGCGTGACGTACAGCGCGTGTAAAGACAACAGTATAAGAATCGGTTCCAA330.10703512698258247No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGGTGGACAGGGATGGTAGATGGAT330.10703512698258247No Hit
CGCGTGACGTACAGCGCGTGTACAGCAAAAGCAGGAGTTTAAAATGAATC330.10703512698258247No Hit
CGCGTGACGTACAGCGCGTGTAAAAACATAATGGACTCCAACACCATGTC320.10379163828614059No Hit
CGCGTGACGTACAGCGCGTGTACGCGTGATCAGCAAAAGCAGGCGTTTAA320.10379163828614059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAG30300.094.996758
ACGTACA30250.094.839737
CGTGACG30600.094.68633
GCGTGAC30550.094.685812
GTGACGT30500.094.6852954
TGACGTA30450.094.6847845
GACGTAC30350.094.683756
GTACAGC30800.093.45469
CGCGTGA31450.091.972741
GGGCAAT357.7603545E-847.4983826-27
GTGTATG650.047.4983818-19
GTGTATA357.7603545E-847.4983818-19
GTGTAGC1950.047.4983818-19
GTGTACG1950.047.4983818-19
GTGTACC1250.047.4983818-19
TAAGCAG700.047.4983820-21
TACGGGG357.7603545E-847.4983820-21
GTACAGG550.047.4983820-21
TAAGAGG357.7603545E-847.4983820-21
GCGTGTA30050.047.4983816-17
Produced by FastQC (version 0.11.5)