FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59447
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGACGTACACTCTAGTATCCTGACCACTCATTTATTCCTACGATATC4090.6880078052719228No Hit
CGCTGACGTACACTCTAGTATCCTACGATATCTTGCTTTATTGAGAAGTT3890.6543643918111932No Hit
CGCTGACGTACACTCTAGTATCCAGAGCAGAAATGAAAAGTGGCGAGAGC2580.4340000336434135No Hit
CGCTGACGTACACTCTAGTATCGGGGGGCAAATGGAGTAAAAGGATTTTC2460.41381398556697563No Hit
CGCTGACGTACACTCTAGTATCGGGGGAGCAAATGGAGTAAAAGGATTTT2230.37512406008713645No Hit
CGCTGACGTACACTCTAGTATCATGCGAACAATTCAACAGACACTGTAGA2180.366713206721954No Hit
CGCTGACGTACACTCTAGTATCCTGCGATATCTTGCTTTATTGAGAAGTT2070.34820932931855264No Hit
CGCTGACGTACACTCTAGTATCCTGAGCACTCATTTATTCCTACGATATC1920.3229767692230054No Hit
CGCTGACGTACACTCTAGTATCCCGACCAGTGAGTACCCTTCCCTTTCAA1670.2809225023970932No Hit
CGCTGACGTACACTCTAGTATCATGACCACTCATTTATTCCTACGATATC1620.2725116490319108No Hit
CGCTGACGTACACTCTAGTATCGGGGAGCAAATGGAGTAAAAGGATTTTC1620.2725116490319108No Hit
CGCTGACGTACACTCTAGTATCATTCGCTTCTGGTAGGCCTGCAAATTTT1610.2708294783588743No Hit
CGCTGACGTACACTCTAGTATCAGGCACTCCTTCCGTAGAAGGCCCTCTT1410.2371860648981446No Hit
CGCTGACGTACACTCTAGTATCAGGCCCCCTCAAAGCCGAGATCGCGCAG1370.23045738220599862No Hit
CGCTGACGTACACTCTAGTATCCCGCACTCAGAATGAAGTGGATGATGGC1370.23045738220599862No Hit
CGCTGACGTACACTCTAGTATCCGGACCACTCATTTATTCCTACGATATC1360.22877521153296215No Hit
CGCTGACGTACACTCTAGTATCGGGGGGGCAAATGGAGTAAAAGGATTTT1270.21363567547563375No Hit
CGCTGACGTACACTCTAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGA1230.20690699278348781No Hit
CGCTGACGTACACTCTAGTATCGCGGGGCAAATGGAGTAAAAGGATTTTC1220.20522482211045132No Hit
CGCTGACGTACACTCTAGTATCAAAATCAGATTGAAACATGCAATCAAAG1210.20354265143741482No Hit
CGCTGACGTACACTCTAGTATCCAGCCAGCAATGTTACATTTACCCAAAT1060.17831009134186754No Hit
CGCTGACGTACACTCTAGTATCCTGCCCACTCATTTATTCCTACGATATC1030.17326357932275807No Hit
CGCTGACGTACACTCTAGTATCATGCGAACAGAGGTTATAAGAATGATGG1020.1715814086497216No Hit
CGCTGACGTACACTCTAGTATCAGTAGCCCTGCAGTGGGACACTTCTGCT1020.1715814086497216No Hit
CGCTGACGTACACTCTAGTATCATGCACGTGTTATCGCATTTGTGGTAAA980.16485272595757566No Hit
CGCTGACGTACACTCTAGTATCATGCCCACTTGCTACTGCAAGCCCATAC940.15812404326542973No Hit
CGCTGACGTACACTCTAGTATCCATAGCCTTTGCCGTAGTGCTAGCCAGC890.1497131899002473No Hit
CGCTGACGTACACTCTAGTATCACCCCCCTTCAATGAAGCCGGCAATGGC890.1497131899002473No Hit
CGCTGACGTACACTCTAGTATCCCTCCCAGCGACTGAACCCCATGCACCA880.1480310192272108No Hit
CGCTGACGTACACTCTAGTATCAGTCGAAATGAATGCCCCTAATTATTAC880.1480310192272108No Hit
CGCTGACGTACACTCTAGTATCGAGGAGCAAATGGAGTAAAAGGATTTTC870.14634884855417432No Hit
CGCTGACGTACACTCTAGTATCCGGAGCAGAAATGAAAAGTGGCGAGAGC870.14634884855417432No Hit
CGCTGACGTACACTCTAGTATCAGGGAGCAAATGGAGTAAAAGGATTTTC850.14298450720810132No Hit
CGCTGACGTACACTCTAGTATCAACATCCACAGCACTCTGCTGTTCCTGT850.14298450720810132No Hit
CGCTGACGTACACTCTAGTATCTATGTTGACAAAATGACCATCGTCAACA840.14130233653506485No Hit
CGCTGACGTACACTCTAGTATCCCGCTATATCCTGACCACTCATTTATTC820.13793799518899186No Hit
CGCTGACGTACACTCTAGTATCTCGGCTTTGAGGGGGCCTGACGGGATGA810.1362558245159554No Hit
CGCTGACGTACACTCTAGTATCGCGGAGCAAATGGAGTAAAAGGATTTTC800.1345736538429189No Hit
CGCTGACGTACACTCTAGTATCGGGGGGAAAATGGACGAAGGACAAGGGT800.1345736538429189No Hit
CGCTGACGTACACTCTAGTATCCGAAGAAATAAGACCCTTCATTACTCAT800.1345736538429189No Hit
CGCTGACGTACACTCTAGTATCCGACGATATCTTGCTTTATTGAGAAGTT770.12952714182380945No Hit
CGCTGACGTACACTCTAGTATCCTGCCACTCATTTATTCCTACGATATCT760.12784497115077295No Hit
CGCTGACGTACACTCTAGTATCGAGGGGCAAATGGAGTAAAAGGATTTTC740.12448062980469998No Hit
CGCTGACGTACACTCTAGTATCCTACGCTATCTTGCTTTATTGAGAAGTT730.12279845913166351No Hit
CGCTGACGTACACTCTAGTATCGGGGCAACATCTTCTCCTATTTCATCAA720.12111628845862701No Hit
CGCTGACGTACACTCTAGTATCCTCTCGTCATTGCAGCAAATATCATTGG720.12111628845862701No Hit
CGCTGACGTACACTCTAGTATCGCGGGAGCAAATGGAGTAAAAGGATTTT710.11943411778559052No Hit
CGCTGACGTACACTCTAGTATCCAACCGGTCTCCTATATGAACTGCTAGG700.11775194711255405No Hit
CGCTGACGTACACTCTAGTATCAAGGTCTCTAATCGGTTGAAGATTACAC680.11438760576648108No Hit
CGCTGACGTACACTCTAGTATCGGAGGAGCAAATGGAGTAAAAGGATTTT670.11270543509344458No Hit
CGCTGACGTACACTCTAGTATCCAACACCATGTCAAGCTTTCAGGTAGAC630.10597675240129863No Hit
CGCTGACGTACACTCTAGTATCCTGAGCATGGCACCCATAATGTTCTCAA620.10429458172826216No Hit
CGCTGACGTACACTCTAGTATCCACCCCCCTTCAATGAAGCCGGCAATGG620.10429458172826216No Hit
CGCTGACGTACACTCTAGTATCCAGCATCGTTTGTTTTGCTCCAGAGGGT620.10429458172826216No Hit
CGCTGACGTACACTCTAGTATCGGCGGAGCAAATGGAGTAAAAGGATTTT610.10261241105522566No Hit
CGCTGACGTACACTCTAGTATCATTACCAACACTACGTCCCCTTGCCCAA610.10261241105522566No Hit
CGCTGACGTACACTCTAGTATCCCGTTATGCTTGTCTTCTAGAAGGTTAA600.10093024038218919No Hit
CGCTGACGTACACTCTAGTATCCGGAGAAATAAGACCCTTCATTACTCAT600.10093024038218919No Hit
CGCTGACGTACACTCTAGTATCATTGGGATCTTGCACCTGATATTGTGGA600.10093024038218919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAT254.5043453E-795.218571
CGCTGAC57850.095.053971
ACGTAGA353.237801E-1094.978126
TGACGGA353.237801E-1094.978124
GAGGTAC156.5928383E-494.978115
TAAACTC201.7321685E-594.978119
TGACATA156.5928383E-494.978114
ACGTAAA201.7321685E-594.978116
ACATACA156.5928383E-494.978116
GACGTAA254.5728302E-794.978115
GTGACGT156.5928383E-494.978113
TGAGGTA156.5928383E-494.978114
GGTACAC156.5928383E-494.978117
ATACACT156.5928383E-494.978118
GTAAACT201.7321685E-594.978118
TGATGTA254.5728302E-794.978114
GCTGACG58000.094.8143542
CTGACGT57800.094.81383
TGACGTA57800.094.81384
ACGTACA57400.094.8126456
Produced by FastQC (version 0.11.5)