FastQCFastQC Report
Mon 1 Apr 2019
HTKZQN1rc_l01_n01_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1rc_l01_n01_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118731
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTACGCTGTCGTCGCGATCTCGGCTTTGAGGGGGCCTGACGGGATG11650.9812096251189664No Hit
CGTCGTACGCTGTCGTCGCGATCAAAGCGTCATTACTTATGAAAACAACA9610.809392660720452No Hit
CGTCGTACGCTGTCGTCGCGATAAGAGGGCAACCCACAGTGTCACTGTTT8410.7083238581330907No Hit
CGTCGTACGCTGTCGTCGCGATAGGCCCCCTCAAAGCCGAGATCGCGCAG7250.6106240156319748No Hit
CGTCGTACGCTGTCGTCGCGATCGGGTTCGTTGCCTTTTCGTCCGAGAGC5420.45649409168624877No Hit
CGTCGTACGCTGTCGTCGCGATAACCGAGGTCGAAACGTACGTTCTTTCT4440.373954569573237No Hit
CGTCGTACGCTGTCGTCGCGATCGGCAAAAGCAGGAGTTTAAAATGAATC4410.37142784950855295No Hit
CGTCGTACGCTGTCGTCGCGATCAGCAAAAGCAGGGTGACAAAAACATAA3760.3166822481070656No Hit
CGTCGTACGCTGTCGTCGCGATCGGCAAAAGCAGGGTGACAAAAACATAA3630.30573312782676804No Hit
CGTCGTACGCTGTCGTCGCGATCGGCAAAAGCAGGTAGATATTTAAAGAT3560.29983744767583864No Hit
CGTCGTACGCTGTCGTCGCGATCTGCAAAAGCAGGAGTTTAAAATGAATC3220.2712012869427529No Hit
CGTCGTACGCTGTCGTCGCGATCTGCAAAAGCAGGGTGACAAAAACATAA3100.2610944066840168No Hit
CGTCGTACGCTGTCGTCGCGATCCAGCCATCTGTTCCATAGCCTTTGCCG2880.24256512620966725No Hit
CGTCGTACGCTGTCGTCGCGATCAGCAAAAGCAGGAGTTTAAAATGAATC2830.2383539261018605No Hit
CGTCGTACGCTGTCGTCGCGATCAGCAAAAGCAGGTAGATATTTAAAGAT2810.2366694460587378No Hit
CGTCGTACGCTGTCGTCGCGATAGGCCCTCTTTGCGTGCGATTGGACCAG2720.22908928586468572No Hit
CGTCGTACGCTGTCGTCGCGATCCACCCCCCTTCAATGAAGCCGGCAATG2690.22656256580000167No Hit
CGTCGTACGCTGTCGTCGCGATCGGAAGAGCACACGTCTGAACTCCAGTC2400.20213760517472268Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CGTCGTACGCTGTCGTCGCGATCTGCAAAAGCAGGTAGATATTTAAAGAT2270.19118848489442522No Hit
CGTCGTACGCTGTCGTCGCGATTGTGGTAAAATTCAAAGCAGCCGTTTCC2150.1810816046356891No Hit
CGTCGTACGCTGTCGTCGCGATGAGTCTTCTAACCGAGGTCGAAACGTAC2000.16844800431226892No Hit
CGTCGTACGCTGTCGTCGCGATCAGTAGAAACAAGGTAGTTTTTTACTCT1960.1650790442260235No Hit
CGTCGTACGCTGTCGTCGCGATGAGGACCCCAACTGCATTTTTGACATCC1940.16339456418290085No Hit
CGTCGTACGCTGTCGTCGCGATGGAGGGGGAAAATGGACGAAGGACAAGG1880.1583411240535328No Hit
CGTCGTACGCTGTCGTCGCGATTGGTGGTAGTAGCCATTTGTCTGTGAGA1850.15581440398884874No Hit
CGTCGTACGCTGTCGTCGCGATGAAAAGGGAAAGACACCCACGGTCGATT1790.15076096385948068No Hit
CGTCGTACGCTGTCGTCGCGATAGAAGGGGAAAACAAAAGCAACAAAAAT1720.14486528370855126No Hit
CGTCGTACGCTGTCGTCGCGATAGGAGGGGAAAACAAAAGCAACAAAAAT1670.14065408360074452No Hit
CGTCGTACGCTGTCGTCGCGATCTACGCGTGATCAGCAAAAGCAGGGTGA1600.13475840344981513No Hit
CGTCGTACGCTGTCGTCGCGATGGGGGACAAAAACATAATGGACTCCAAC1590.1339161634282538No Hit
CGTCGTACGCTGTCGTCGCGATAGGCACCAAACGATCATATGAACAAATG1540.12970496332044706No Hit
CGTCGTACGCTGTCGTCGCGATAGCAGGGGAAAACAAAAGCAACAAAAAT1470.12380928316951766No Hit
CGTCGTACGCTGTCGTCGCGATAGGACCAGGCGGTCATGGAAAAGAACAT1470.12380928316951766No Hit
CGTCGTACGCTGTCGTCGCGATGGGGGGCAAAAACATAATGGACTCCAAC1360.11454464293234286No Hit
CGTCGTACGCTGTCGTCGCGATACGCGTGATCAGTAGAAACAAGGTAGTT1360.11454464293234286No Hit
CGTCGTACGCTGTCGTCGCGATAACAGGGGAAAACAAAAGCAACAAAAAT1350.1137024029107815No Hit
CGTCGTACGCTGTCGTCGCGATAAGGGGAAAACAAAAGCAACAAAAATGA1340.11286016288922017No Hit
CGTCGTACGCTGTCGTCGCGATATTCCCCAAGACAAGTTCATGGCCCAAT1320.11117568284609747No Hit
CGTCGTACGCTGTCGTCGCGATCTGCACTCCCATTCGCTTCTGGTAGGCC1320.11117568284609747No Hit
CGTCGTACGCTGTCGTCGCGATCGGACCAGGCGGTCATGGAAAAGAACAT1310.11033344282453614No Hit
CGTCGTACGCTGTCGTCGCGATCATCCCGTCAGGCCCCCTCAAAGCCGAG1290.10864896278141344No Hit
CGTCGTACGCTGTCGTCGCGATGAAAAGGGAAAGATAGTCAAATCAGTCG1270.10696448273829076No Hit
CGTCGTACGCTGTCGTCGCGATGAAACGAGAAAGCTCTTATCTCTTGTTC1250.10528000269516807No Hit
CGTCGTACGCTGTCGTCGCGATAAGAGGGGAAAACAAAAGCAACAAAAAT1240.10443776267360673No Hit
CGTCGTACGCTGTCGTCGCGATTTGCAGACAATGGATTGGGTGATGCCCC1230.10359552265204537No Hit
CGTCGTACGCTGTCGTCGCGATGGGAGTGCAGATGCAGCGATTCAAGTGA1230.10359552265204537No Hit
CGTCGTACGCTGTCGTCGCGATCAGTAATCCACAATATCAGGTGCAAGAT1210.1019110426089227No Hit
CGTCGTACGCTGTCGTCGCGATGGTGGGGAGCGCCAGGATGCCACAGAAA1210.1019110426089227No Hit
CGTCGTACGCTGTCGTCGCGATTTGTGGTAAAATTCAAAGCAGCCGTTTC1200.10106880258736134No Hit
CGTCGTACGCTGTCGTCGCGATGGGAGGCTGGTGTTTATAGCACCCTTGG1190.10022656256580001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTCG156.593061E-495.036669
GGTCGTA254.5844536E-795.036661
ACGTTGT254.5844536E-795.036667
ACGCAGT254.5844536E-795.036667
CGTTGTC254.5844536E-795.036668
GCAGTCG254.5844536E-795.036669
CGCTGGC254.5844536E-795.036668
ACGATGT156.593061E-495.036667
CGGTGTC156.593061E-495.036668
ACGCTGG254.5844536E-795.036667
CATCGTA254.5844536E-795.036661
CGTCGGA201.7340379E-595.036661
CGTCGAA156.593061E-495.036661
ACGGTGT156.593061E-495.036667
ACGCGGT156.593061E-495.036667
GGTACGC409.094947E-1294.996634
CGAACGC156.604119E-494.996634
TACGCGG156.604119E-494.996636
TACGCAG201.737672E-594.996636
TACGATG156.604119E-494.996636
Produced by FastQC (version 0.11.5)