FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13250888
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT177740.13413440669032897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT326700.091.84561
CGCGTCA247750.090.916196
GCGCACT259150.090.729257
TGTCGCG263300.090.436443
GCGCGTC252100.090.32725
CGCGCAC261400.090.2390446
CGTGTCG485300.090.2339861
TCGCGCG256900.090.119353
CGCGCGT254350.089.9210054
CGACTAT316750.089.796981
CGAGATG199950.089.7505651
GTGTCGC269250.089.743432
CGTCGTA188300.089.7032241
CGAGTAG185700.089.322111
TCACGTC313650.088.7157443
CACGTCG311200.088.712074
GTGTCGT221350.087.5971762
GCGTCAC257800.087.150847
CGCTATC142300.086.989361
CGCTACA91050.086.966561
Produced by FastQC (version 0.11.5)