FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC379CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC379CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40649
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT37099.124455706167433No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGGGTTTT31027.631184038967747No Hit
TGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT4431.089817707692686No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTATTTT3460.85118945115501No Hit
CGAGACGATGTGTACGTACTACCCGGGAACAACTAGGTCAGAAGTTTGAA2980.7331053654456444No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGTAGTTTT2540.6248616202120593No Hit
CGAGACGATGTGTACGTACTACCCGCGCTGGGATGTTCCTCTAGATATTT2300.5658195773573765No Hit
CGAGACGATGTGTACGTACTACGCGGGATCAGTAGAAACAAGGGGGTTTT1890.4649560874806269No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGGATTTT1610.39607370415016363No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGTCGTTTT1520.37393293807965755No Hit
CGAGACGATGTGTACGTACTACAAGGGTGTTTTTTATCACTAAATAAGCT1480.3640925976038771No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGGGGTTT1470.361632512484932No Hit
CGAGACGATGTGTACGTACTACAAGGGAACAACTAGGTCAGAAGTTTGAA1460.35917242736598687No Hit
CGAGACGATGTGTACGTACTACAAGGGCAGAAGTTTGAAGAAGTAAGATG1440.3542522571280966No Hit
CGAGACGATGTGTACGTACTACGGGGGAACAACTAGGTCAGAAGTTTGAA1290.3173509803439199No Hit
CGAGACGATGTGTACGTACTACGGGGCGGGAACAACTAGGTCAGAAGTTT1250.3075106398681394No Hit
CAAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT1210.29767029939235895No Hit
CGAGACGATGTGTACGTACTACTAGGTCAGAAGTTTGAAGAAGTAAGATG1170.28782995891657853No Hit
CGAGACGATGTGTACGTACTACCGGGGCAGAAGTTTGAAGAAGTAAGATG1140.2804497035597432No Hit
CGAGACGATGTGTACGTACTACGGGGGCAGAAGTTTGAAGAAGTAAGATG1040.25584885237029203No Hit
CGAGACGATGTGTACGTACTACGGGGGGGGAAGTCTTTGAGCTCTCGGAC1040.25584885237029203No Hit
CGAGACGATGTGTACGTACTACCAGGGCAGAAGTTTGAAGAAGTAAGATG1020.25092868213240177No Hit
CGAGACGATGTGTACGTACTACAGGGCGGGAACAACTAGGTCAGAAGTTT1020.25092868213240177No Hit
CGAGACGATGTGTACGTACTACGAGGGCAGAAGTTTGAAGAAGTAAGATG980.24108834165662132No Hit
CGAGACGATGTGTACGTACTACCGGGCGGGAACAACTAGGTCAGAAGTTT930.22878791606189575No Hit
CGAGACGATGTGTACGTACTACGCGGGATCAGTAGAAACAAGGGTGTTTT900.2214076607050604No Hit
CGAGACGATGTGTACGTACTACCAGGCGGGAACAACTAGGTCAGAAGTTT800.19680680951560925No Hit
CGAGACGATGTGTACGTACTACAGGGGCAGAAGTTTGAAGAAGTAAGATG790.19434672439666414No Hit
CGAGAAGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT790.19434672439666414No Hit
CGAGACGATGTGTACGTACTACGCGTGTCAGTAGAAACAAGGGTGTTTTT710.1746660434451032No Hit
CGAGACGATGTGTACGTACTACGCGTGTCAGTAGAAACAAGGGGGTTTTT670.16482570296932275No Hit
CGAGACGATGTGTACGTACTACCCGGGGACAACTAGGTCAGAAGTTTGAA670.16482570296932275No Hit
CGAGACGATGTGTACGTACTACGAGGGAACAACTAGGTCAGAAGTTTGAA670.16482570296932275No Hit
CGAGACGATGTGTACGTACTACGGGGGGGGAACAACTAGGTCAGAAGTTT650.1599055327314325No Hit
TGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGGGTTTT650.1599055327314325No Hit
CGAGACGATGTGTACGTACTACTACGCGTGATCAGTAGAAACAAGGGTGT650.1599055327314325No Hit
TGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTATTTT630.15498536249354228No Hit
CGAGACGATGTGTACGTACTACCCAGGTGCTGTTTTCATATGTGATGATT620.15252527737459715No Hit
CAAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGGGTTTT600.14760510713670694No Hit
CGAGACGATGTGTACGTACTACAAGGCGGGAACAACTAGGTCAGAAGTTT590.1451450220177618No Hit
CGAGACGATGTGTACGTACTACAGGGGGAAAATTGATGGAGTGAAATTGG590.1451450220177618No Hit
CGAGACGATGTGTACGTACTACTACGCGTGATCAGTAGAAACAAGGGGGT580.1426849368988167No Hit
CGAGACGATGTGTACGTACTACCCGGCGGGAACAACTAGGTCAGAAGTTT550.13530468154198136No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGAGTTTT540.13284459642303623No Hit
CGAGACGATGTGTACGTACTACCCGGGCAGAAGTTTGAAGAAGTAAGATG530.13038451130409112No Hit
CGAGACGATGTGTACGTACTACCAGGGAACAACTAGGTCAGAAGTTTGAA530.13038451130409112No Hit
CGAGACGATGTGTACGTACTACAAGGGGGTTTTTTATCACTAAATAAGCT520.12792442618514602No Hit
CGAGACGATGTGTACGTACTACCTCAGCATTGTCTCCGAAGAAATAAGAT500.12300425594725577No Hit
CGAGACGATGTGTACGTACTACGCGTGGTCAGTAGAAACAAGGGGGTTTT490.12054417082831066No Hit
AGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT490.12054417082831066No Hit
CGAGACGATGTGTACGTACTACGCGTGCTCAGTAGAAACAAGGGTGTTTT490.12054417082831066No Hit
CGAGACGATGTGTACGTACTACAGGGGCAGAAATCATAAAGATGATGGAA480.11808408570936554No Hit
CGAAACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT470.11562400059042043No Hit
CGAGACGATGTGTACGTACTACGCGGGATCAGTAGAAACAAGGGGGGTTT460.11316391547147532No Hit
CGAGACGATGTGTACGTACTACGCGCGATCAGTAGAAACAAGGGTGTTTT460.11316391547147532No Hit
CGAGACGATGTGTACGTACTACGCGTGGTCAGTAGAAACAAGGGTGTTTT460.11316391547147532No Hit
CGAGACGATGTGTACGTACTACGGGGGGAGAATGGAAGAAAAACAAGGAT440.10824374523358507No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGCGTTTT440.10824374523358507No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAACAAGGGTGTTTTT430.10578366011463998No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAAAAAGGGGGTTTT420.10332357499569485No Hit
CGAGACGATGTGTACGTACTACAGGTGCTGTTTTCATATGTGATGATTCT420.10332357499569485No Hit
TGAGACGATGTGTACGTACTACCCGGGAACAACTAGGTCAGAAGTTTGAA410.10086348987674973No Hit
CGAGACGATGTGTACGTACTACCCGGGAGCAACTAGGTCAGAAGTTTGAA410.10086348987674973No Hit
CGAGACGATGTGTACGTACTACGCGTGCTCAGTAGAAACAAGGGGGTTTT410.10086348987674973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCGA201.7235538E-595.000012
CGATATG254.5403067E-795.000016
GATATGT254.5403067E-795.000017
ATGATGT254.5403067E-795.000015
ACCATGT254.5403067E-795.000015
CCAGACG201.7235538E-595.000011
ATGTGGA500.095.000018
AAGATGT500.095.000015
CGGGACG409.094947E-1295.000011
AGATGTG500.095.000016
GACGATA201.7235538E-595.000014
TGTGGAC450.095.000019
ACGATAT201.7235538E-595.000015
CGAGACC254.5403067E-795.000011
CGAGACA450.095.000011
GACCATG254.5403067E-795.000014
TGTGTAG201.7235538E-595.000019
GAGATGA254.5403067E-795.000012
TGAGACG2750.095.01
AACGATG301.2021701E-895.04
Produced by FastQC (version 0.11.5)