FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC348CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC348CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGAGACACTACGCAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCAC191.4264264264264264No Hit
CGTCGAGACACTACGCAGCGTCAGTAGAAACAAGGTCGTTTTTAAATTAT90.6756756756756757No Hit
CGTCGAGACACTACGCATCATCACCATTGTTGGCTTGGCGCCAAATCCGC60.45045045045045046No Hit
CATCGAGACACTACGCAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCAC60.45045045045045046No Hit
CGTCGAGACACTACGCAGCGTCATACTCCATATTTTTGGCTGGACCAATT50.37537537537537535No Hit
TGTCGAGACACTACGCAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCAC50.37537537537537535No Hit
CGTCGAGACACTACGCAGCGTCCTACTCCATATTTTTGGCTGGACCAATT40.3003003003003003No Hit
CGTCGAGACACTACGCAGAGTCCAGTAGTAATTGATTCTTCCTTCTTGAT40.3003003003003003No Hit
CGTCGAGACACTACGCAGCGTCAGAAGAAACAAGGTCGTTTTTAAATTAT40.3003003003003003No Hit
CCTCGAGACACTACGCAGCGTCATACTCCATATTTTTGGCTGGACCAATT30.22522522522522523No Hit
CATCGAGACACTACGCAGCGTCATACTCCATATTTTTGGCTGGACCAATT30.22522522522522523No Hit
CGTCGAGACACTACGCAGCGTCGAAAGGGGAAAAAGCTAATGTGCTAATT30.22522522522522523No Hit
CGTCGAGACACTACGCAGCGTCAGGCCATGGAGGTTGCTAGTCAGGCCAG30.22522522522522523No Hit
CGTCGAGACACTACGCAGCGTCATCACCATTGTTGGCTTGGCGCCAAATC30.22522522522522523No Hit
CCTCGAGACACTACGCAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCAC20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCCATAGCCTTAGCTGTAGTGCTGGCCAGA20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCGGGCAAAGAAGATAGAAGATATGGGCCA20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCGGGGGGGAAAAAGCTAATGTGCTAATTG20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAGTAGAAACAAGGTAGTTTTTTACTCTA20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAAAAGGGGAAAAAGCTAATGTGCTAATT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCCAGTAGTAATTGATTCTTCCTTCTTGAT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCGGGGGAAAAAGCTAATGTGCTAATTGGG20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCCC20.15015015015015015No Hit
CATCGAGACACTACGCAGCGTCAGTAGAAACAAGGTCGTTTTTAAATTAT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCGAACGGGCAAATGGTAACAACAACCAAT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAGGAGAAACAAGGTACTTTTTTGGACAG20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCGGAAGGGGAAAAAGCTAATGTGCTAATT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCCACAGCATTCTGCTGTTCCTCTCGATAT20.15015015015015015No Hit
CGTCGAGACACTACGCATCATCACCATTGTTGGCTTGGCGCCAAATCCCC20.15015015015015015No Hit
CGTCGAGACACTACGCAGAGGCCCTTTGTTGATTAGTGTTCCCTCCACTT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAGGCCCCCTCAAGATCGGGATTCTTTCC20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCCAAAGGGGAAAAAGCTAATGTGCTAATT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAACAACAAAATGATCTCCTTGAAAATTT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCGCCAGGCAAATGGTAACAACAACCAATC20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCATACACCATATTTTTGGCTGGACCAATT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCCAGGCAGATGGTGCAGGCAATGAGAGCC20.15015015015015015No Hit
CGTCGAGACACTACGCAACCTCCATGGCCTCAGCTGCTTGTTCGCTCGAT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCCAGGGGAAAAAGCTAATGTGCTAATTGG20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCCCGCGCTGGGATGTTCCTCTAGATATTT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCGGGACAGGAACAGGATACACCATGGATA20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAGAAGCTTGAAGAAGTAAGATGGCTGAT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCTTTGCCTAAAATGAGGAATCCCCTTAGA20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCATTAGATTTCCATTTGCCTCAAATATTA20.15015015015015015No Hit
CTTCGAGACACTACGCAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCAC20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCCCCAGGCAAATGGTAACAACAACCAATC20.15015015015015015No Hit
CGTCGAGACACTACCCAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCAC20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCCCTCTATTATCTCAAATCTGTGCTTTAA20.15015015015015015No Hit
CGTCGAGACACTACGCAGCTTATTTAGTGATAAAAACGACCTTGTTTCTA20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAAACCCCTTTCTAAGTCTGTTACTTTTG20.15015015015015015No Hit
TGTCGAGACACTACGCAGCGTCGAAAGGCAAATGGTAACAACAACCAATC20.15015015015015015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGA800.095.000012
CGTCGAG800.095.000011
ACACTAC1250.095.08
GACACTA1250.095.07
CGAGACA1100.095.04
ATCGAGA305.16593E-995.02
AGACACT1250.095.06
TCGAGAC1100.095.03
CATCGAG305.16593E-995.01
CACTACG1300.094.999999
GAGACAC1150.094.999995
GCGTCCA204.4513764E-447.50000418-19
AGCGTCC402.0863808E-947.50000416-17
CGTCAGT150.00921297147.518-19
ACGCAGC1200.047.512-13
TACGCAG1200.047.512-13
CGCAGCG1200.047.514-15
GCGTCCC150.00921297147.518-19
GCGTCAG354.591857E-847.518-19
CTACGCA1200.047.510-11
Produced by FastQC (version 0.11.5)