FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC346CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC346CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348247
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTATATACTCTAGCGAGTGGCAAGGACAAGACAAATCCTAAAATCCC50671.455001765987934No Hit
CGTGTATATACTCTAGCGAGTGGCCGAGACAAGACAAATCCTAAAATCCC49591.4239892949544433No Hit
CGTGTATATACTCTAGCGAGTGGCCAAGACAAGACAAATCCTAAAATCCC43841.2588766019520627No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCGAAAGACAAGACAAATCCTAA37931.0891694687965725No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCCAAAGACAAGACAAATCCTAA32220.9252053858324694No Hit
CGTGTATATACTCTAGCGAGTGGCGCAGACAAGACAAATCCTAAAATCCC31940.9171651155645275No Hit
CGTGTATATACTCTAGCGAGTGATTAGCAAAAGCAGGTAGATATTGAAAG30070.8634675962750576No Hit
CGTGTATATACTCTAGCGAGTGGCAAAGGCAAGACAAATCCTAAAATCCC29640.8511200383635753No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCAAAAGACAAGACAAATCCTAA29560.8488228182870203No Hit
CGTGTATATACTCTAGCGAGTGATTAGCGAAAGCAGGTAGATATTGAAAG25630.735971882026263No Hit
CGTGTATATACTCTAGCGAGTGAACAGCAAAAGCAGGTAGATATTGAAAG22760.6535591117798574No Hit
CGTGTATATACTCTAGCGAGTGGCGAAGACAAGACAAATCCTAAAATCCC22620.6495389766458864No Hit
CGTGTATATACTCTAGCGAGTGGCGACGACAAGACAAATCCTAAAATCCC22400.6432216214353605No Hit
CGTGTATATACTCTAGCGAGTGAACAGCGAAAGCAGGTAGATATTGAAAG22310.6406372488492363No Hit
CGTGTATATACTCTAGCAAGTGCGGAGGCCATGGTCATTTTAAGTGCCTC21810.6262796233707685No Hit
CGTGTATATACTCTAGCGAGTGGCACAGACAAGACAAATCCTAAAATCCC21560.6191008106315345No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGTAGATATTGAAAG21030.6038817276243585No Hit
CGTGTATATACTCTAGCGAGTGATCAGCGAAAGCAGGTAGATATTGAAAG19700.565690443851634No Hit
CGTGTATATACTCTAGCGAGTGAGGCCCCCTCAAAGCCGAGATCGCACAG18020.5174488222439819No Hit
CGTGTATATACTCTAGCGAGTGCGGAGGCCATGGTCATTTTAAGTGCCTC17570.5045269593133609No Hit
CGTGTATATACTCTAGCGAGTGGCGGAGACAAGACAAATCCTAAAATCCC16880.48471343615307527No Hit
CGTGTATATACTCTAGCGAGTGGCCAGGACAAGACAAATCCTAAAATCCC15690.4505422875143218No Hit
CGTGTATATACTCTAGCGAGTGACCAGCGAAAGCAGGTAGATATTGAAAG14940.42900584929661995No Hit
CGTGTATATACTCTAGCGAGTGGCGAGGACAAGACAAATCCTAAAATCCC13300.3819128377272453No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCAAAGACAAGACAAATCCTAAA12840.3687038222870549No Hit
CGTGTATATACTCTAGCGAGTGCAGCGAAAGCAGGTAGATATTGAAAGAT12800.36755521224877746No Hit
CGTGTATATACTCTAGCGAGTGATAAGCGAAAGCAGGTAGATATTGAAAG12750.36611944970093063No Hit
CGTGTATATACTCTAGCGAGTGGCGGCGACAAGACAAATCCTAAAATCCC12690.3643965346435145No Hit
CGTGTATATACTCTAGCGAGTGACCAGCAAAAGCAGGTAGATATTGAAAG12390.3557819593564338No Hit
CGTGTATATACTCTAGCGAGTGATAAGCAAAAGCAGGTAGATATTGAAAG12350.3546333493181564No Hit
CGTGTATATACTCTAGCGAGTGGCCCAGACAAGACAAATCCTAAAATCCC12100.34745453657892245No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCAAAAGCAGGTAGATATTGAAAG11480.3296510809856223No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCGAAAGCAGGTAGATATTGAAAG11450.3287896234569142No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCAAATGCAACAACAAGAGGATC11210.3218979632272496No Hit
CGTGTATATACTCTAGCGAGTGCTCAGCAAAAGCAGGTAGATATTGAAAG10790.3098375578253366No Hit
CGTGTATATACTCTAGCGAGTGGCAGAGACAAGACAAATCCTAAAATCCC10480.30093583002868657No Hit
CGTGTATATACTCTAGCGAGTGATGAGCAAAAGCAGGTAGATATTGAAAG10070.2891625771363429No Hit
CGTGTATATACTCTAGCGAGTGATGAGCGAAAGCAGGTAGATATTGAAAG9810.2816966118875396No Hit
CGTGTATATACTCTAGCGAGTGGCGCCGACAAGACAAATCCTAAAATCCC9560.27451779914830565No Hit
CGTGTATATACTCTAGCGAGTGGCAACGACAAGACAAATCCTAAAATCCC9450.27135912154304276No Hit
CGTGTATATACTCTAGCGAGTGGAGAGGCAAAAGACAAGACAAATCCTAA8770.25183275089232643No Hit
CGTGTATATACTCTAGCGAGTGGCCGGGACAAGACAAATCCTAAAATCCC8690.24953553081577157No Hit
CGTGTATATACTCTAGCGAGTGGAGAGGCGAAAGACAAGACAAATCCTAA8400.2412081080382602No Hit
CGTGTATATACTCTAGCGAGTGGGGGTGCAGATGCAACGATTCAAGTGAT8300.23833658294256663No Hit
CGTGTATATACTCTAGCGAGTGGCAAAGACAAGACAAATCCTAAAATCCC8270.23747512541385857No Hit
CGTGTATATACTCTAGCGAGTGGAAAAGACAAGACAAATCCTAAAATCCC7890.22656333005022297No Hit
CGTGTATATACTCTAGCGAATGGGGGTGCAGATGCAACGATTCAAGTGAT7810.2242661099736681No Hit
CGTGTATATACTCTAGCGAGTGGAGGGCAAAAGACAAGACAAATCCTAAA7790.22369180495452942No Hit
CGTGTATATACTCTAGCGAGTGGGAAAGACAAGACAAATCCTAAAATCCC7780.22340465244496005No Hit
CGTGTATATACTCTAGCGAGTGGAGGGCGAAAGACAAGACAAATCCTAAA7300.2096213319856309No Hit
CGTGTATATACTCTAGCGAGTGATCCGCAAAAGCAGGTAGATATTGAAAG7280.20904702696649216No Hit
CGTGTATATACTCTAGCGAGTGCGGCAACGATTCAAGTGATCCTCTTGTT7210.20703695939950664No Hit
CGTGTATATACTCTAGCGAGTGATCAACAAAAGCAGGTAGATATTGAAAG7200.20674980688993733No Hit
CGTGTATATACTCTAGCGAGTGGGGCAGGGAATGAGTCCCAATGGCTCTC7110.2041654343038131No Hit
CGTGTATATACTCTAGCGAGTGATCCGCGAAAGCAGGTAGATATTGAAAG6960.19985814666027274No Hit
CGTGTATATACTCTAGCGAGTGCTCAGCGAAAGCAGGTAGATATTGAAAG6960.19985814666027274No Hit
CGTGTATATACTCTAGCGAGTGATCAACGAAAGCAGGTAGATATTGAAAG6940.19928384164113402No Hit
CGTGTATATACTCTAGCGAGTGAAAGCAGGAAGATATTGAAAGATGAGTC6820.19583801152630176No Hit
CGTGTATATACTCTAGCGAGTGGGGGGCTATATGAAGCAATTGAGGAGTG6640.1906692663540533No Hit
CGTGTATATACTCTAGCGAGTGCCTAAAATGAGGAATCCCCTTAGAACCG5810.16683560805979664No Hit
CGTGTATATACTCTAGCGAGTGGGGGGCTATAAGAAGCATTAAGCAAAAC5620.16137971037797885No Hit
CGTGTATATACTCTAGCGAGTGATCATCAAAAGCAGGTAGATATTGAAAG5620.16137971037797885No Hit
CGTGTATATACTCTAGCGAGTGGAGGCGGCAACCTGTGAACAGATTGCCG5620.16137971037797885No Hit
CGTGTATATACTCTAGCGAGTGGCCAAGGCAAGACAAATCCTAAAATCCC5600.16080540535884014No Hit
CGTGTATATACTCTAGCGAGTGGCAGGGACAAGACAAATCCTAAAATCCC5570.15994394783013208No Hit
CGTGTATATACTCTAGCGAGTGGGAAAGGCAAGACAAATCCTAAAATCCC5540.159082490301424No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCGAATGCAACAACAAGAGGATC5470.1570724227344385No Hit
CGTGTATATACTCTAGCGAGTGATCATCGAAAGCAGGTAGATATTGAAAG5370.1542008976387449No Hit
CGTGTATATACTCTAGCGAGTGATGGGTTTTTCTCCTGTCTTCCTGATGT5310.15247798258132877No Hit
CGTGTATATACTCTAGCGAGTGCGGGCAATGAGTCCCAATGGCTCTCATT5170.14845784744735777No Hit
CGTGTATATACTCTAGCGAGTGGGGTGCAGATGCAACGATTCAAGTGATC4970.1427147972559706No Hit
CGTGTATATACTCTAGCGAGTGGCGAAGGCAAGACAAATCCTAAAATCCC4920.14127903470812384No Hit
CGTGTATATACTCTAGCGAGTGGAGGGCCAAAGACAAGACAAATCCTAAA4850.13926896714113832No Hit
CGTGTATATACTCTAGCGAGTGGGGAAGACAAGACAAATCCTAAAATCCC4820.13840750961243026No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCGAAAGACAAGACAAATCCTAA4750.13639744204544474No Hit
CGTGTATATACTCTAGCGAGTGGGGCGGGCAATGAGTCCCAATGGCTCTC4630.13295161193061247No Hit
CGTGTATATACTCTAGCGAGTGGAGAGGCCAAAGACAAGACAAATCCTAA4630.13295161193061247No Hit
CGTGTATATACTCTAGCGAGTGCAGCAAAAGCAGGTAGATATTGAAAGAT4570.13122869687319633No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCGAAAGACAACACAAATCCTAA4460.1280700192679334No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAGAAACAAGGTCGTTTTTAAAT4460.1280700192679334No Hit
CGTGTATATACTCTAGCGAGTGCGGTGCTCCAGTTTCGGTATTTTTTGTC4310.12376273162439304No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCATCTGCACCCCCATTCGTTTC4250.12203981656697688No Hit
CGTGTATATACTCTAGCGAGTGACCAGCGAAAGCAGGTCAATTATATTCA4010.1151481563373123No Hit
CGTGTATATACTCTAGCGAGTGGCGGGGGCAAGACAAAACCTAAAAACCC3720.10682073355980096No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCAAATGCAACGATTCAAGTGAT3680.10567212352152351No Hit
CGTGTATATACTCTAGCGAGTGGCCAAAGCAAGACAAATCCTAAAATCCC3640.10452351348324608No Hit
CGTGTATATACTCTAGCGAGTGGACAAAGCGTCTACGCTGCAGTCCTCGC3520.10107768336841379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT1250.095.01
ATAATCT450.095.08
TACGCTA156.61402E-495.09
ATATACG156.61402E-495.06
CGTGTAG301.2294549E-895.01
CGTGTAC254.6138484E-795.01
CGTGTAA301.2294549E-895.01
GTATAGA500.095.04
TAAATAC301.2294549E-895.05
ATACGCT156.61402E-495.08
TATACGC156.61402E-495.07
TACACTA156.61402E-495.09
TAATCTA450.095.09
TATATGC409.094947E-1294.999995
TATATCC201.7421158E-594.999995
TGTATCT201.7421158E-594.999993
TATGCTC409.094947E-1294.999997
GTATCTA201.7421158E-594.999994
TACTCTG409.094947E-1294.999999
TGCTCTA409.094947E-1294.999999
Produced by FastQC (version 0.11.5)