HTKZQN1_l02_n02_BC345CG_2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HTKZQN1_l02_n02_BC345CG_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41420
Sequences flagged as poor quality	0
Sequence length	101
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACT	975	2.3539352969579914	No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGGGTTTTTTATCACT	580	1.4002897151134719	No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTCGTTTTTAAATTAT	563	1.3592467407049735	No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTATTTTTCATTAAT	393	0.9488169966199904	No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTAGTTTTTTACTCTA	360	0.8691453404152583	No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGGTGTTTTTTATCACT	241	0.5818445195557701	No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGTCGTTTTTAAATTAT	204	0.49251569290197966	No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGGATTTTTCATTAAT	177	0.42732979237083535	No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGGGGTTTTTTATCACT	164	0.39594398841139544	No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGGTATTTTTCATTAAT	156	0.3766296475132786	No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGTAGTTTTTTACTCTA	144	0.34765813616610336	No Hit
CGAGACGTAGATGACGAGTATCCAGGCGGGAACAACTAGGTCAGAAGTTT	138	0.3331723804925157	No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTCCTTATA	132	0.318686624818928	No Hit
TGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACT	127	0.306615161757605	No Hit
CGAGACGTAGATGACGAGTATCGGGGCGGGAACAACTAGGTCAGAAGTTT	121	0.2921294060840174	No Hit
CGAGACGTAGATGACGAGTATCCGGGCGGGAACAACTAGGTCAGAAGTTT	121	0.2921294060840174	No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTACTTTTTTGGACAG	106	0.2559150169000483	No Hit
CGAGACGTAGATGACGAGTATCACTAAATAAGCTGAAACGAAAACGTTCT	97	0.23418638338966682	No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGGGTTTTTCCTTATA	94	0.22694350555287301	No Hit
CGAGACGTAGATGACGAGTATCTACGCGTGATCAGTAGAAACAAGGGGGT	89	0.21487204249154998	No Hit
CGAGACGTAGATGACGAGTATCCCGCGCTGGGATGTTCCTCTAGATATTT	87	0.21004345726702076	No Hit
CGAGACGTAGATGACGAGTATCTACGCGTGATCAGTAGAAACAAGGGTGT	82	0.19797199420569772	No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGGGATTTTTCATTAAT	74	0.17865765330758088	No Hit
CGAGACGTAGATGACGAGTATCGAGGCGGGAACAACTAGGTCAGAAGTTT	66	0.159343312409464	No Hit
CGAGACGTAGATGACGAGTATCGAGGGCGGAAATCATAAAGATGATGGAA	65	0.15692901979719942	No Hit
TGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTATTTTTCATTAAT	64	0.1545147271849348	No Hit
CGAGACGTAGATGACGAGTATCTTGGGGGGCTATATGAAGCAATTGAGGA	63	0.15210043457267022	No Hit
CGAGACGTAGATGACGAGTATCCGTAGAAGGCCCTCTTTTTAAACCGTGT	59	0.14244326412361177	No Hit
TGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTAGTTTTTTACTCTA	58	0.1400289715113472	No Hit
CGAGACGTAGATGACGAGTATCAAGGCGGGAACAACTAGGTCAGAAGTTT	54	0.13037180106228874	No Hit
CGAGACGTAGATGACGAGTATCCAGCCATTTGCTCCATAGCCTTAGCTGT	53	0.12795750845002415	No Hit
CGAGACGTAGATGACGAGTATCAGGAGAAACAAGGGTGTTTTTTATCACT	53	0.12795750845002415	No Hit
CGAGACGTAGATGACGAGGATCAGTAGAAACAAGGGGGTTTTTTATCACT	53	0.12795750845002415	No Hit
CGAGACGTAGATGACGAGTATCGAAAGGGGAAAAAGCTAATGTGCTAATT	53	0.12795750845002415	No Hit
CGAGACGTAGATGACGAGTATCCCGGCGGGAACAACTAGGTCAGAAGTTT	51	0.12312892322549492	No Hit
CGAGACGTAGATGACGAGTATCGGGGGCAGAAATCATAAAGATGATGGAA	50	0.12071463061323032	No Hit
CGAGACGTAGATGACGAGTATCAGGAGAAACAAGGTCGTTTTTAAATTAT	48	0.11588604538870112	No Hit
CGAGACGTAGATGACGAGTATCCGAAGAAATAAGATCCTTCATTACTCAT	48	0.11588604538870112	No Hit
CGAGACGTAGATGACGAGTATCCCGGGAACAACTAGGTCAGAAGTTTGAA	47	0.11347175277643651	No Hit
CGAGACGTAGATGACGAGTATCAGAAGAAACAAGGGTGTTTTTTATCACT	45	0.10864316755190728	No Hit
CGAGACGTAGATGACGAGTATCCAGAGTCTATGAGGGAAGAATATCGAGA	45	0.10864316755190728	No Hit
CGAGACGTAGATGACGAGTATCGAGGGCAGAAGTTTGAAGAAGTAAGATG	45	0.10864316755190728	No Hit
CGAGACGTAGATGACGAGTATCGAGAGCGGAAGTTTCGTCCAACATCCTG	45	0.10864316755190728	No Hit
CGAGACGTAGATGACGAGTATCCGAAGAATCAAAGTTAAACAGGGAGAAA	44	0.10622887493964268	No Hit
CGAGACGTAGATGACGAGTATCGCGGGGACAACTAGGTCAGAAGTTTGAA	43	0.10381458232737809	No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGCGTTTTTAAATTAT	43	0.10381458232737809	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATGAC	20	1.7239585E-5	95.00001	9
GTAGACG	40	9.094947E-12	95.00001	7
ACATGAC	20	1.7239585E-5	95.00001	9
GTACATG	20	1.7239585E-5	95.00001	7
ACGTACA	20	1.7239585E-5	95.00001	5
CGTAGAC	40	9.094947E-12	95.00001	6
GACGCAG	20	1.7239585E-5	95.00001	4
GACGTAC	20	1.7239585E-5	95.00001	4
CGTACAT	20	1.7239585E-5	95.00001	6
GTGACGT	20	1.7239585E-5	95.00001	2
GGGACGT	20	1.7239585E-5	95.00001	2
CAGATGA	20	1.7239585E-5	95.00001	8
CGTGACG	20	1.7239585E-5	95.00001	1
TGACGTA	20	1.7239585E-5	95.00001	3
TAGACGA	40	9.094947E-12	95.00001	8
GGACGTA	20	1.7239585E-5	95.00001	3
GAGACGC	20	1.7239585E-5	95.00001	2
AGATAAC	20	1.7239585E-5	95.00001	9
CGCGACG	15	6.572561E-4	95.0	1
GAGGCGT	35	3.2014214E-10	95.0	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content