FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC337CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC337CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152401
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGAGTAGTACAGTGTCTGAAACGAAAACGTTCTAATCTCTTGTTCCA21611.4179696983615593No Hit
CGTAGAGTAGTACAGTGTCTGACCGAGAGTGCTGCCTCTTCCCTTTAGAG6250.41010229591669345No Hit
CGTAGAGTAGTACAGTGTCTGAAGAAACAAGGTCGTTTTTAAATTATTCG6020.3950105314269591No Hit
CGTAGAGTAGTACAGTGTCTGATGGCCATCCGAATTCTTTTGGTCGCTGT5440.35695303836589No Hit
CGTAGAGTAGTACAGTGTCTGACCTAGTTGTTCCCGCCATTTTCCGTTTC5360.3517037289781563No Hit
CGTAGAGTAGTACAGTGTCTGACAGCGACCAAATTAATTGATGGCCATCC4750.31167774489668704No Hit
CGTAGAGTAGTACAGTGTCTGAATGAGCGGGTCATTATGGTGAAACATGA4670.3064284355089534No Hit
CGTAGAGTAGTACAGTGTCTGACTTAGCCAACAGTGTCCTGCATACTTTC4620.3031476171416198No Hit
CGTAGAGTAGTACAGTGTCTGACCCAGCTATCTCAATGCATGTGTTAGGA4320.2834627069376185No Hit
CGTAGAGTAGTACAGTGTCTGATTGAGGAAGTGAGGCATAAATGTTATTT4050.2657462877540174No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGCGGGAACAACTAGGTCAGAAGTTT3820.25065452326428306No Hit
CGTAGAGTAGTACAGTGTCTGAGGTCTTCAATGGTGGAACAGATCTTCAT3770.2473737048969495No Hit
CGTAGAGTAGTACAGTGTCTGAAAAAGCAATGAAAGAGTATGGAGAGGAC3540.2322819404072152No Hit
CGTAGAGTAGTACAGTGTCTGACGCGTGATCAGTAGAAACAAGGTCGTTT3270.21456552122361403No Hit
CGTAGAGTAGTACAGTGTCTGACGCGTGATCAGTAGAAACAAGGGTGTTT3250.2132531938766806No Hit
CGTAGAGTAGTACAGTGTCTGAAAGGGGTTTGAGATGATTTGGGATCCTA3220.21128470285628045No Hit
CGTAGAGTAGTACAGTGTCTGACTCAGCTGAAAATCCTTCTAATTGTGGA3110.20406690244814665No Hit
CGTAGAGTAGTACAGTGTCTGATAGGCCTGTAAATTTTCAAGGAGATCAT3060.2007860840808131No Hit
CGTAGAGTAGTACAGTGTCTGAATGCCAGATCATAATGTGAGTCAAACCA3040.19947375673387968No Hit
CGTAGAGTAGTACAGTGTCTGACGGGGTACAAACCATTCTTCCCCGTCAG3020.19816142938694625No Hit
CGTAGAGTAGTACAGTGTCTGAAAACCCTTTTACTCCGTTTGCTCCATCA3020.19816142938694625No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGGCGAAGACATAATGGATTCCCAC2960.19422444734614602No Hit
CGTAGAGTAGTACAGTGTCTGAAGGCCCTCTTTTTAAACCGTGTTTAAAG2910.19094362897881248No Hit
CGTAGAGTAGTACAGTGTCTGAAGAAGAATCCGATGAGGCACTTAAAATG2890.18963130163187905No Hit
CGTAGAGTAGTACAGTGTCTGACGCGTGATCAGTAGAAACAAGGTACTTT2470.16207242734627728No Hit
CGTAGAGTAGTACAGTGTCTGAAAGAGAATCCGGAGGCCATGGTCATTTT2410.158135445305477No Hit
CGTAGAGTAGTACAGTGTCTGATTGGTCAGGATGTTGGACGAAACTTCCG2400.15747928163201028No Hit
CGTAGAGTAGTACAGTGTCTGACGCGTGATCAGTAGAAACAAGGTAGTTT2400.15747928163201028No Hit
CGTAGAGTAGTACAGTGTCTGAATAAGCTGAAACGAAAACGTTCTAATCT2380.15616695428507688No Hit
CGTAGAGTAGTACAGTGTCTGACTAGCAACCTCCATGGCCTCAGCTGCTT2370.15551079061161016No Hit
CGTAGAGTAGTACAGTGTCTGAGGTCGTTTTTAAATTATTCGAAATTAAT2340.15354229959121002No Hit
CGTAGAGTAGTACAGTGTCTGATTGGAGAAGTGAGGCATAAATGTTATTT2310.1515738085708099No Hit
CGTAGAGTAGTACAGTGTCTGAACCCCCATTCGTTTCTGATAGGCCTGTA2310.1515738085708099No Hit
CGTAGAGTAGTACAGTCTCTGTGCGATCTCGGCTTTGAGGGGGCCTGACG2280.1496053175504098No Hit
CGTAGAGTAGTACAGTGTCTGAAAGCGCATGCCATGATGGCATGGGCTGG2280.1496053175504098No Hit
CGTAGAGTAGTACAGTGTCTGAATGAGCTACTTGGGAAGAATTTTTCAAA2280.1496053175504098No Hit
CGTAGAGTAGTACAGTGTCTGAAGGGCGGGAACAACTAGGTCAGAAGTTT2240.14698066285654293No Hit
CGTAGAGTAGTACAGTGTCTGAAAGCCCTTAGTAATGTCAGATTCTCCAA2230.14632449918307622No Hit
CGTAGAGTAGTACAGTGTCTGAACAATGAGGAGCAACTTTCTTGACTCAG2130.13976286244840913No Hit
CGTAGAGTAGTACAGTGTCTGAAGAAACAAGGTACTTTTTTGGACAGTAT2050.13451355306067547No Hit
CGTAGAGTAGTACAGTGTCTGACTTCCAGTTTGGATTGAGTGACTAGCCC2050.13451355306067547No Hit
CGTAGAGTAGTACAGTGTCTGACCAATCAGTTATTGCTACAACATCCTGT1990.1305765710198752No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGCCTGACGGGATGATAGAGAGAAC1970.12926424367294176No Hit
CGTAGAGTAGTACAGTGCCTCATCGGATTCTTCTTTCAGAATCCTCTCTA1950.12795191632600836No Hit
CGTAGAGTAGTACAGTGTCTGACAAAATGATCATCGTCAGCATCCACAGC1880.12335877061174139No Hit
CGTAGAGTAGTACAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGC1870.1227026069382747No Hit
CGTAGAGTAGTACAGTGTCTGACGTCCCTCATAATCATTGAGCTTAAGCT1860.12204644326480799No Hit
CGTAGAGTAGTACAGTGTCTTTAGCCACTCCATGAGAGCCTCAAGATCGG1690.1108916608158739No Hit
CGTAGAGTAGTACAGTGTCTGAACCCAAATCAAAAGATAATAACCATTGG1660.10892316979547378No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGCTATATGAAGCAATTGAGGAGTG1660.10892316979547378No Hit
CGTAGAGTAGTACAGTGTCAGAGGTGACAGGATTGGTCTTGTCTTTAGCC1640.10761084244854037No Hit
CGTAGAGTAGTACAGTGACTGTTGATGGAGCAAACGGAGTAAAAGGGTTT1620.10629851510160694No Hit
CGTAGAGTAGTACAGTGTCTGAACCAGCCGTTGCATCATCACCATTGTTG1590.10433002408120683No Hit
CGTAGAGTAGTACAGTGTCTGACAGAGCAGTCAAACTTTATCGAAAGCTT1560.10236153306080668No Hit
CGTAGAGTAGTACAGTGTCTGAGAAACGAAAACGTTCTAATCTCTTGTTC1550.10170536938733997No Hit
CGTAGAGTAGTACAGTGTCTGAGAGCTCAAAGACTCCCCGCCCCTGGAAG1550.10170536938733997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC156.606821E-495.07
TAGTTCA1850.095.08
TATTACA156.606821E-495.08
TAGTGTA301.2247256E-895.03
CGGAGAG254.6013156E-795.01
AGTACGG156.606821E-495.09
GGTAGTA353.274181E-1095.06
AGTGTAG301.2247256E-895.04
TAATACA201.7389588E-594.999998
CGTATAG201.7389588E-594.999991
GTAATAC201.7389588E-594.999997
CGTAGTA201.7389588E-594.999996
CGTAGGG409.094947E-1294.999991
CGTAGCG201.7389588E-594.999991
CGTACAG201.7389588E-594.999991
TAGGACA409.094947E-1294.999998
GCGTAGT201.7389588E-594.999995
GTAGTAA409.094947E-1294.999997
TAGTACA147050.093.966348
AGTACAG148400.093.943739
Produced by FastQC (version 0.11.5)