FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC333CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC333CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGACGACTACGCGAGACACCTGGGACCTTTGATCTTGGGGGGCTATA80.6557377049180327No Hit
CGACGACGACTACGCGAGACACATTAGCTTTTTCCCCTTTCGCAAGGTTG70.5737704918032787No Hit
CGACGACGACTACGCGAGACACCCAAGATCAAAGGTCCCAGGTTCCAGAT50.4098360655737705No Hit
CGACGACGACTACGCGAGACACAGAAACGAAAACGTTCTAATCTCTTGTT50.4098360655737705No Hit
CGACGACGACTACGCGAGACACTGAAACGAAAACGTTCTAATCTCTTGTT30.24590163934426232No Hit
CGACGACGACTACGCGAGACACCATTGTTGGCTTGGCGCCAAATCCGCCT30.24590163934426232No Hit
CGACGACGACTACGCGAGACACAAAGGGGAAAAAGCTAATGTGCTAATTG30.24590163934426232No Hit
CGACGACGACTACGCGAGACACAATATCAAGTGCACAATCCCAATTATAC30.24590163934426232No Hit
CGACGACGACTACGCGAGACACGAAAGGGGAAAAAGCTAATGTGCTAATT30.24590163934426232No Hit
CGACGACGACTACGCGAGACACCTCACCCACTTTCCATCTACTCTCTTGT30.24590163934426232No Hit
CGACGACGACTACGCGAGACACAGAAGGGGAAAAAGCTAATGTGCTAATT30.24590163934426232No Hit
CGACGACGACTACGCGAGACACCAGGGACCTTTGATCTTGGGGGGCTATA20.16393442622950818No Hit
CGACGACGACTACGCGAGACACCTTGCCCAATTAGCACATTAGCTTTTTC20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAGGCACATGGTAACAACAACCAATCCAT20.16393442622950818No Hit
CGACGACGACTACGCGAGACACGAGGGGAAAAAGCTAATGTGCTAATTGG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACGGGGGCAGAAGTTTGAAGAAGTAAGATG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAGAAACCGAAAACGTTCTAATCTCTTGT20.16393442622950818No Hit
CGACGACGACTACGCGAGACACCCGAGATCAAAGGTCCCAGGCTCTTAGG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAAAAGAAATGCTGCTCCCACTAGTCCAG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACACCAGCCGTTGCATCATCACCATTGTTG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAAGGGCAGAAATCATAAAGATGATGGAA20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAAGAGGATCACTTGAATCGTTGCATCTG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACCCCAAGACAAAGGTCCCAGGTTCCTCAT20.16393442622950818No Hit
CGACGACGACTACGCGAGACACACGAAACGAAAACGTTCTAATCTCTTGT20.16393442622950818No Hit
CGACGACGACTACGCGAGACACATTCCCATTAAGGGCATTTTGGACAAAG20.16393442622950818No Hit
TGACGACGACTACGCGAGACACCCAAGGTCGATTTGAACCATGCCAGTTG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAAGGGTGTTTTTTATCACTAAATAAGCT20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAGGCAAGGTCTTATACAGTCCAATCCTG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACGAAAACGTTCTAATCTCTTGTTCCACTT20.16393442622950818No Hit
CGACGACGACTACGCGAGACACGAAACGAAAACGTTCTAATCTCTTGTTC20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAGTAGAAACAAGGTACTTTTTTGGACAG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACACTACATCCCCTTGCCCAATTAGCACAT20.16393442622950818No Hit
CGACGACGACTACGCGAGACACATGCCATAATCAAGAAATACACATCAGG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACACAGGAGAAACAAGGTACTTTTTTGGAC20.16393442622950818No Hit
CGACGACGACTACGCGAGACACTTGGGGGGCTATATGAAGCAATTGAGGA20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAAGAGGGAAATAACATTCCATGGGGCCA20.16393442622950818No Hit
CGACGACGACTACGCGAGACACGGAAACGAAAACGTTCTAATCTCTTGTT20.16393442622950818No Hit
CGACGACGACTACGCGAGACACGAGCCAACAATTGCTCCCTCTTCGGTGA20.16393442622950818No Hit
CGACGACGACTACGCGAGACACCCAAGAAAGTTGCTCCTCATTGTTCAGG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAGCCCCCATCCTGTTGTATATGAGTCCC20.16393442622950818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT1050.095.000015
ACTACGC1050.095.000019
GACTACG1050.095.000018
ACGACTA1100.095.06
CGACTAC1100.095.07
ACGACGA1150.090.869573
GACGACG1100.090.6818162
CGACGAC2100.047.5000044
TACGCGA1100.047.510-11
CTACGCG1100.047.510-11
CGAGACA1150.047.514-15
ACGCGAG1100.047.512-13
AGACACG252.0665126E-547.516-17
AGACACC354.375579E-847.516-17
AGACACA458.54925E-1147.516-17
GAGACAC1100.047.516-17
CGCGAGA1100.047.512-13
GACACGA204.3785325E-447.49999618-19
ACACAGG150.00912341947.49999618-19
GACACCC150.00912341947.49999618-19
Produced by FastQC (version 0.11.5)