FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC331CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC331CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4556
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACTCTCGCATAGACTACTACAGCTCTATGCTGACAAAATGACCATCG631.382791922739245No Hit
CGTACTCTCGCATAGACTACTAAGGCCCTCCTTTCAGTCCGTATTTAAAG601.3169446883230904No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGTAGTTT601.3169446883230904No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGTCTATCA531.163301141352063No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGGTGTTT521.141352063213345No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGCCTATCA380.8340649692712907No Hit
CGTACTCTCGCATAGACTACTACCGCCCCTGGAAAGACACATCTTCTGGT300.6584723441615452No Hit
CGTACTCTCGCATAGACTACTACGGCTCTATGCTGACAAAATGACCATCG270.5926251097453907No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGCAGTTT270.5926251097453907No Hit
CGTACTCTCGCATAGACTACTAATCCGCTTGTTCTCTTAAATGTGAATCC260.5706760316066725No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGGGGTTT260.5706760316066725No Hit
CGTACTCTCGCATAGACTACTAAGGCACTCCTTCCGTAGAAGGCCCTCCT250.5487269534679543No Hit
CGTACTCTCGCATAGACTACTACGCCCCTGGAAAGACACATCTTCTGGTC220.4828797190517998No Hit
CGTACTCTCGCATAGACTACTAAGCACCTCTTCCTCTCTCTTGACTGATG170.3731343283582089No Hit
CGTACTCTCGCATAGACTACTACGGCACTCCTTCCGTAGAAGGCCCTCCT150.3292361720807726No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGCGTTTT120.2633889376646181No Hit
CGTACTCTCGCATAGACTACTACGCCGCTGGAAAGACACATCTTCTGGTC120.2633889376646181No Hit
CGTACTCTCGCATAGACTACTAAGCCGCTTGTTCTCTTAAATGTGAATCC120.2633889376646181No Hit
CGTACTCTCGCATAGACTACTAGGGGGCCAAATCAGCATACAACCTACGT110.2414398595258999No Hit
CGTACTCTCGCATAGACTACTAGGGGGGGGAAGTGAGGAATGATGATGTT110.2414398595258999No Hit
CGTACTCTCGCATAGACTACTAATTAGCCTAATATTGCAAATAGGGAATA100.21949078138718175No Hit
CGTACTCTCGCATAGACTACTAGAAGGCCCTCCTTTCAGTCCGTATTTAA90.19754170324846357No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGTCGTTT90.19754170324846357No Hit
CGTACTCTCGCATAGACTACTACGGCCCTGGAAAGACACATCTTCTGGTC90.19754170324846357No Hit
CGTACTCTCGCATAGACTACTAAGGAGCTGAGGGAGCAATTGAGCTCAGT90.19754170324846357No Hit
CGTACTCTCGCATAGACTACTACCGCGCCTGGAAAGACACATCTTCTGGT80.17559262510974538No Hit
CGTACTCTCGCATAGACTACTAACGCGTGATCAGTAGAAACAAGGTAGTT80.17559262510974538No Hit
CGTACTCTCGCATAGACTACTAAGGCGCTCCTTTCAGTCCGTATTTAAAG80.17559262510974538No Hit
CGTACTCTCGCATAGACTACTACGGCCACCAAAACTGAAGGATGAGCTAA70.15364354697102722No Hit
CGTACTCTCGCATAGACTACTACGGCCCCTGGAAAGACACATCTTCTGGT70.15364354697102722No Hit
CGTACTCTCGCATAGACTACTACGGCGCTGGAAAGACACATCTTCTGGTC70.15364354697102722No Hit
CGTACTCTCGCATAGACTACTCCAGCTCTATGCTGACAAAATGACCATCG70.15364354697102722No Hit
CGTACTCTCGCATAGACTACTAGGGCACTCCTTCCGTAGAAGGCCCTCCT60.13169446883230904No Hit
CGTACTCTCGCATAGACTACTACTGCCCCTGGAAAGACACATCTTCTGGT60.13169446883230904No Hit
CGTACTCTCGCATAGACTACTAAGGCCAATAAAATTAAATCTGAGAAAAC60.13169446883230904No Hit
CGTACTCTCGCATAGACTACTAGGGGCGGGAACAATTAGGTCAGAAGTTT60.13169446883230904No Hit
CGTACTCTCGCATAGACTACTATGCGTGATCAGCAAAAGCAGGCCTATCA60.13169446883230904No Hit
CGTACTCTCGCATAGACTACTACGGCCCTCCTTTCAGTCCGTATTTAAAG60.13169446883230904No Hit
CGTACTCTCGCATAGACTACTAAAGAAACGAATGGGGGTGCAGATGCAAC50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAAGGGGGGGAAGTGAGGAATGATGATGTT50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTACCCACCAAAACTGAAGGATGAGCTAATT50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGCCGTTT50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTACGTGTGATCAGTAGAAACAAGGGTGTTT50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAACGCGTGATCAGCAAAAGCAGGCCTATC50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTATTGGCACTCCTTCCGTAGAAGGCCCTCC50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAATGCACTCTTATCTTCAATGTTTGAAGA50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTACGTCCCTGGAAAGACACATCTTCTGGTC50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAATGGGGGTGCAGATGCAACGGTTCAAGT50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTACGCCTCTGGAAAGACACATCTTCTGGTC50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAAGGCGCTCCTTCCGTAGAAGGCCCTCCT50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAATGACCATCGTCAGCATCCACAGCACTC50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAAGGCGCCAAAACTGAAGGATGAGCTAAT50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTACGGGGGGGAAGTGAGGAATGATGATGTT50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAGGGGGGGGAACAATTAGGTCAGAAGTTT50.10974539069359088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4350.095.000019
ACTCTCG4100.095.04
TCGCATA4300.095.08
CTCGCAT4250.094.999997
GTACTCT4350.092.816092
CGTACTC4350.092.816091
TCTCGCA4300.092.7906956
TACTCTC4300.091.686043
CTCTCGC4650.083.763445
CTACAGC451.7826096E-1047.50000420-21
AGACTAC4350.047.50000414-15
TAGACTA4350.047.50000412-13
TACTAGG451.7826096E-1047.50000418-19
ACTAAGG252.61047E-547.518-19
GTTCTTC252.61047E-547.594-95
CTGGAAA356.823575E-847.528-29
ATAGACT4400.047.512-13
ACTAGGG301.3345816E-647.518-19
ACTAGCG301.3345816E-647.518-19
CTACTAG1000.047.516-17
Produced by FastQC (version 0.11.5)