FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC330CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC330CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8550
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTATATAGTACTGTCTCTGCACACACACATCACTTTGCCGGTATCAG690.8070175438596492No Hit
CGAGTATATAGTACTGTCACTACAGAGACATAAGCATTTTCATTCTGATA650.7602339181286549No Hit
CGAGTATATAGTACCGTCGCTTTAAATACGGACTGAAAGGAGGGCCTTCT430.5029239766081871No Hit
CGAGTATATAGTACTGTCACTGTCACATTCTTCTCGAGTACTGTGTCAAC370.43274853801169594No Hit
CGAGTATATAGTACTGTCGCTGGGGGGCTATATGAAGCAATTGAGGAGTG270.3157894736842105No Hit
CGAGTATATAGTACTGTCGCTGATTCGCCTTATTTCTTCTTTGTCATAAA230.2690058479532164No Hit
CGAGTATATAGTACTGTCGCTGATGATCCGCTTGTTCTCTTAAATGTGAA210.2456140350877193No Hit
CGAGTATATAGTACTGTCTCTGTAGTGACTTCAAATTATAACAGGAGATT210.2456140350877193No Hit
CGAGTATATAGTACTGTCTCCGGGTTTTAGCAAGGTCCAGTAATAGTTCA200.23391812865497078No Hit
CGAGTATATAGTACTGTCGCATTCTGGGTTTCCCAAGAGCCATCCGGCGA190.2222222222222222No Hit
CGAGTATATAGTACTGTCGCTGATCATAGAAACAAGGGTGTTTTTTATTA160.18713450292397663No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAAGATCCTAAGAAAACTGG160.18713450292397663No Hit
CGAGTATATAGTACTGTCGCTGAAACGAGAAAGTTCTTATCTCTTGCTCC150.17543859649122806No Hit
CGAGTATATAGTACTGTCGCTGAAGATAACAGAGAATAGTTTTGAGCAAA150.17543859649122806No Hit
CGAGTATATAGTACTGTCGCGTGATCAGTAGAAACAAGGTAGTTTTTTAC120.14035087719298245No Hit
CGAGTATATAGTACTGCAGCTGCAAGTGCACTTAACAGGACCAGTAGGTT120.14035087719298245No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGAAAGATCCTAAGAAAACTGGA110.1286549707602339No Hit
CGAGTATATAGTACTGTCGCTGCGGCTCCAGACCTCCTAGGGAGAGTTGA110.1286549707602339No Hit
CGAGTATATAGTACTGTCGCTGAAACCATAAAAAGTTGGAGGAAGAAAAT110.1286549707602339No Hit
CGAGTATATAGTACTGTCGCTGATCCGCTTGTTCTCTTAAATGTGAATCC110.1286549707602339No Hit
CGAGTATATAGTACTGTCGCTGATCATAGAAACAAGGTAGTTTTTTACTC110.1286549707602339No Hit
CGAGTATATAGTACTGTCGCTGCACACACACATCACTTTGCCGGTATCAG100.11695906432748539No Hit
CGAGTATATAGTACTGTCGCTGAGTCCCGTTTCCGTTTCATTACCAACAC100.11695906432748539No Hit
CGAGTATATAGTACTGTCGCTGCTCCACTTCAAGCAATAGATGTAAGGCT100.11695906432748539No Hit
CGAGTATATAGTACTGTTGCTCTTCTCCCAACCATTGTGAACTCTTCATA100.11695906432748539No Hit
CGAGTATATAGTACTGTCGCTGCGCACACACATCACTTTGCCGGTATCAG100.11695906432748539No Hit
CGAGTATATAGTACTGTCGCTGGCGGGAACAATTAGGTCAGAAGTTTGAA90.10526315789473684No Hit
CGAGTATATAGTACTGTCGCTGACTCACATGATGATCTGGCATTCCAATT90.10526315789473684No Hit
CGAGTATATAGTACTGTCACAGCCCCCATCCTGTTGTATATGAGGCCCAT90.10526315789473684No Hit
CGAGTATATAGTACTGTCGCTGATGATCTGGCATTCCAATTTGAATGATG90.10526315789473684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG156.3936005E-495.05
AGGATAT156.3936005E-495.03
GGATATA156.3936005E-495.04
AGTATAT7800.095.03
GAGTATA7750.095.02
ATAGTAC8150.095.08
CGAGTAT7750.095.01
ATATAGT8150.095.06
GAGGATA201.6463053E-594.999992
CGAGGAT201.6463053E-594.999991
CGGGTAT201.6463053E-594.999991
TAGTACT8100.094.999999
CGTGTAT201.6463053E-594.999991
GGTATAT201.6463053E-594.999993
GTATATA8250.094.424244
TATAGTA8250.094.424247
TATATAG8200.094.420745
GGGTATA254.98156E-576.02
TGTATAT254.98156E-576.03
GTGTATA301.229072E-463.3333362
Produced by FastQC (version 0.11.5)