FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC324CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC324CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10362
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCAGTCTCTATACGTCTAGCCGGCGCCATTCTGTTTTCATGCCTGATT920.8878594865856012No Hit
CGTCAGTCTCTATACGTCTAGCCGGCACCATTCTGTTTTCATGCCTGATT640.6176413819725921No Hit
CGTCAGTCTCTATACGTCTAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA610.588689442192627No Hit
CGTCAGTCTCTATACGTCTAGCCAGCACCATTCTGTTTTCATGCCTGATT440.42462845010615713No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGGGAACAGTGACCACAGAAGCTG400.3860258637328701No Hit
CGTCAGTCTCTATACGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTTT370.3570739239529048No Hit
CGTCAGTCTCTATACGTCTAGCCGCCCAAGATCGAAGGTTCCAGGTTCCA320.30882069098629605No Hit
CGTCAGTCTCTATACGTCTAGCCCGACCAGTGAGTACCCTTCCCTTTCAA300.2895193977996526No Hit
CGTCAGTCTCTATACGTCTAGCCAGCGCCATTCTGTTTTCATGCCTGATT300.2895193977996526No Hit
CGTCAGTCTCTATACGTCTAGCCGGCCCCATTCTGTTTTCATGCCTGATT280.2702181046130091No Hit
CGTCAGTCTCTATACGTCTAGCCTGCGCCATTCTGTTTTCATGCCTGATT260.25091681142636557No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGGGAAAATGGACGAAGGACAAGG250.24126616483304383No Hit
CGTCAGTCTCTATACGTCTAGCCCGCACCATTCTGTTTTCATGCCTGATT220.21231422505307856No Hit
CGTCAGTCTCTATACGTCTAGCCCGCCAAGATCGAAGGTTCCAGGTTCCA220.21231422505307856No Hit
CGTCAGTCTCTATACGTCTAGCAAGCCCATACACACAAGCAGGCAGGCAG200.19301293186643506No Hit
CGTCAGTCTCTATACGTCTAGCCTGACCACTCATTTATTCCTACGATATC170.1640609920864698No Hit
CGTCAGTCTCTATACGTCTAGCCGGCCCAGTGAGTACCCTTCCCTTTCAA170.1640609920864698No Hit
CGTCAGTCTCTATACGTCCAGCAGCAAAGTTGGTGTTGCTGATGTTAACA160.15441034549314803No Hit
CGTCAGTCTCTATACGTCTACGCGTGATCAGTAGAAACAAGGGGGTTTTT160.15441034549314803No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGCCAAGGAGGTGTCACTAAGCTA160.15441034549314803No Hit
CGTCAGTCTCTATACGTCTAGCCGGCAATGTTACATTTACCCAAATGCAA160.15441034549314803No Hit
CGTCAGTCTCTATACGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCAC160.15441034549314803No Hit
CGTCAGTCTCTATACGTCTAGCCCGCGCCATTCTGTTTTCATGCCTGATT150.1447596988998263No Hit
CGTCAGTCTCTATACGTCTAGCGAGGGGGGAAAATGGACGAAGGACAAGG150.1447596988998263No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGAAAATGGACGAAGGACAAGGGT150.1447596988998263No Hit
CGTCAGTCTCTATACGTCTAGCCCGCCCCTGGAAGGACAAATCTTCTGGC150.1447596988998263No Hit
CGTCAGTCTCTATACGTCTAGCAGGCCCATACACACAAGCAGGCAGGCAG140.13510905230650455No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGTTTTGTTCAGCATCCAGAACTAA130.12545840571318279No Hit
CGTCAGTCTCTATACGTCTAGCCCGAGCAGTGAGTACCCTTCCCTTTCAA130.12545840571318279No Hit
CGTCAGTCTCTATACGTCTAGCCCGCCCAGTGAGTACCCTTCCCTTTCAA130.12545840571318279No Hit
CGTCAGTCTCTATACGTCTAGCCGGCCAGTGAGTACCCTTCCCTTTCAAA130.12545840571318279No Hit
CGTCAGTCTCTATACGTCTAGCCAGCAATGTTACATTTACCCAAATGCAA130.12545840571318279No Hit
CGTCAGTCTCTATACGTCTAGCCGCCCCTGGAAGGACAAATCTTCTGGCT130.12545840571318279No Hit
CGTCAGTCTCTATACGTCTAGCCGGCGATGTTACATTTACCCAAATGCAA130.12545840571318279No Hit
CGTCAGTCTCTATACGTCTAGCCGGCGCACAAGCAGGCAGGCAGGATTTA120.11580775911986102No Hit
CGTCAGTCTCTATACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTA120.11580775911986102No Hit
CGTCAGTCTCTATACGTCTAGCCCCACAAGATCGAAGGTTCCAGGTTCCA120.11580775911986102No Hit
CGTCAGTCTCTATACGTCTAGCCATTCCATGAGAGCCTCAAGATCTGTGT120.11580775911986102No Hit
CGTCAGTCTCTATACGTCTAGCCGGCCAAGATCGAAGGTTCCAGGTTCCA120.11580775911986102No Hit
CGTCAGTCTCTATACGTCTAGCAGGGGGGGAACAGTGACCACAGAAGCTG120.11580775911986102No Hit
CGTCAGTCTCTATACGTCTAGCCTGCACCATTCTGTTTTCATGCCTGATT120.11580775911986102No Hit
CGTCAGTCTCTATACGTCTAGCAGGACCTTACTGGCAAAATCTGTATTCA120.11580775911986102No Hit
CGTCAGTCTCTATACGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTG120.11580775911986102No Hit
CGTCAGTCTCTATACGTCTAGCCGCACCATTCTGTTTTCATGCCTGATTA110.10615711252653928No Hit
CGTCAGTCTCTATACGTCTAGCCGTCCAAGATCGAAGGTTCCAGGTTCCA110.10615711252653928No Hit
CGTCAGTCTCTATACGTCTAGCACCCCAAGATCGAAGGTTCCAGGTTCCA110.10615711252653928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCAGT156.432572E-495.01
ATCAGTC156.432572E-495.02
CTCTATA10000.094.5249948
TCTATAC10000.094.5249949
GTCTCTA10000.094.5249946
GTCAGTC9600.094.010422
CGTCAGT9550.094.005231
TCAGTCT9800.093.545923
TCTCTAT10200.093.137257
AGTCTCT10150.092.6600955
CAGTCTC10150.090.788174
AGCCCGA205.265756E-447.50000420-21
GAGGTGT205.265756E-447.50000434-35
ATGGGCC205.265756E-447.50000476-77
TGCATGG205.265756E-447.50000472-73
ACTAAGC205.265756E-447.50000442-43
CAGTTGC205.265756E-447.50000468-69
GCCTCAT205.265756E-447.50000480-81
GTGCACT205.265756E-447.50000458-59
GCAAGGG205.265756E-447.50000420-21
Produced by FastQC (version 0.11.5)