FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC317CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC317CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4597
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGATATAGTCAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC380.8266260604742223No Hit
CGTCGATATAGTCAGAGAGTACGCGTGATCAGTAGAAACAAGGGGGTTTT330.7178594735697195No Hit
CGTCGATATAGTCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTTG260.5655862519034153No Hit
CGTCGATATAGTCAGAGAGTAGCTGAAACGAGAAAGCTCTTATCTCTTGT230.5003262997607135No Hit
CGTCGATATAGTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCT140.3045464433326082No Hit
CGTCGATATAGTCAGAGAGTAGCTGAGCTTTGGGTATGAATTTCCTTTTT130.28279312595170764No Hit
CGTCGATATAGTCAGAGAGAGGAATTGCCCGCTAATTTCACGGAAACCAC130.28279312595170764No Hit
CGTCGATATAGTCAGAGAGTAGAGGCCCATTAGAGCACATCCAGAAACTG110.23928649118990647No Hit
CGTCGATATAGTCAGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGGT90.1957798564281053No Hit
CGTCGATATAGTCAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAG80.1740265390472047No Hit
CGTCGATATAGTCAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAA80.1740265390472047No Hit
CGTCGATATAGTCAGAGAGTAGCCGCGAAGTAGGTACAGATGCAATTGTC70.1522732216663041No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGCAAAAACATAATGGACTCCAAC70.1522732216663041No Hit
CGTCGATATAGTCAGAGAGTAGTTGTAAGGCTTGCATAAATGTTATTTGT70.1522732216663041No Hit
CGTCGATATAGTCAGAGAGTAGAAACAAGGGGGTTTTTTATCATTAAATA60.13051990428540353No Hit
CGTCGATATAGTCAGAGAGTAGAAAGCTCTTATCTCTTGTTCTACTTCAA60.13051990428540353No Hit
CGTCGATATAGTCAGAGAGTAGAAACGGGAAAGCTCTTATCTCTTGTTCT50.10876658690450293No Hit
CGTCGATATAGTCAGAGAGTAGCGGCGCGAAGTAGGTACAGATGCAATTG50.10876658690450293No Hit
CGTCGATATAGTCAGAGAGTAGAAACGAGAAAGCTCTTATCTCTTGTTCT50.10876658690450293No Hit
CGTCGATATAGTCAGAGATAAGAGCTTTCTCGTTTCAGCTTATTTAATGA50.10876658690450293No Hit
CGTCGATATAGTCAGAGAGTACGCGTGATCAGTAGAAACAAGGGTGTTTT50.10876658690450293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCAG156.198759E-495.09
CGATATT156.198759E-495.04
GATATTG156.198759E-495.05
GTCGATA4100.095.02
TATTGTC156.198759E-495.07
TAGTCAG3950.095.09
ATAGTCA3900.095.08
CGTCGAT4000.095.01
ATATTGT156.198759E-495.06
TATAGTC3900.095.07
ATATAGT4000.095.06
CGATATA4050.094.999994
GATATAG4050.094.999995
TCGATAT4150.093.855423
ATTGTCA200.001943417671.258
GAGTAGG900.047.50000416-17
AGAGAGT3600.047.50000414-15
GTAGGGG451.7826096E-1047.50000418-19
GTAGCGG451.7826096E-1047.50000418-19
AGTAGCG700.047.518-19
Produced by FastQC (version 0.11.5)