FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC316CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC316CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATACTCACGCGCGACACGCGTGATCAGTAGAAACAAGGGTGTTTTT60.5253940455341506No Hit
CGACATACTCACGCGCGACACGCGTGATCAGTAGAAACAAGGGGGTTTTT40.3502626970227671No Hit
CGACATACTCACGCGCGACACGAGGCCAAGGGTGTTGCCTCTTCCTTTTA40.3502626970227671No Hit
CGACATACTCACGCGCGACACGAGGCCAAGGGGGTTGCCTCTTCCTTTTA40.3502626970227671No Hit
CGACATACTCACGCGCGACACGGAAACCACTGACTGTCCAGCAGCAAAGT40.3502626970227671No Hit
CGACATACTCACGCGCGACACGAAAACCACTGACTGTCCAGCAGCAAAGT30.2626970227670753No Hit
CGACATACTCACGCGCGACACGATTCAACAGACACTGTAGACACAGTACT30.2626970227670753No Hit
CGACATACTCACGCGCGACACGCAGACCTTCTGGGAAGTGTTGAACCTTG20.17513134851138354No Hit
CGACATACTCACGCGCGACACGACCACCCATTTATTCCTACGATATCTTG20.17513134851138354No Hit
CAACATACTCACGCGCGACACGGAAACCACTGACTGTCCAGCAGCAAAGT20.17513134851138354No Hit
TGACATACTCACGCGCGACACGAGGCCAAGGGTGTTGCCTCTTCCTTTTA20.17513134851138354No Hit
CGACATACTCACGCGCTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTA20.17513134851138354No Hit
CGACATACTCACGCGCGACAGAGAATAGTTTCGAACAAATAACATTTATG20.17513134851138354No Hit
CGACATACTCACGCGCGACACGCCACTATCAAGAGTTGGAGAAACAATAT20.17513134851138354No Hit
CGACATACTCACGCGCGACACGCGCCACAAAAGGATATGCTGCTCCCGCT20.17513134851138354No Hit
CGACATACTCACGCGCGACACGGATGCTCTTCTAGGTATTTATTTCTTCT20.17513134851138354No Hit
CGACATACTCACGCGCGACACGATTCGAGCCATGCCAGTTATCCCTGCAC20.17513134851138354No Hit
CGACATACTCACGCGCGACACGATTCAAAGCAGCCGTTTCCAATTTCCTT20.17513134851138354No Hit
CGACATACTCACGCGCGACACGACGGCCAAGGGTGTTGCCTCTTCCTTTT20.17513134851138354No Hit
CGACATACTCACGCGCGGCACAGATTGAAAGCGACAGAGAATAGTTTCGA20.17513134851138354No Hit
CGACATACTCACGCGCGACACGAGGATAGTTTCGAACAAATAACATTTAT20.17513134851138354No Hit
TGACATACTCACGCGCGACACGCGAAAGCTCTTATCTCTTGTTCTACTTC20.17513134851138354No Hit
CGACATACTCACGCGCGACACGCCTCTCCTTATTTCTTCTTTGTCATAAA20.17513134851138354No Hit
CGTCATACTCACGCGCGACACGCTGACCATCGTCAACATCCACAGCACTC20.17513134851138354No Hit
CGACATACTCACGCGCGACACGACTCGAGCCATGCCAGTTATCCCTGCAC20.17513134851138354No Hit
CGACATACTCACGCGCGACACGAGAAAGCTCTTATCTCTTGTTCTACTTC20.17513134851138354No Hit
CGACATACTCACGCGCGACACGAACAGAGGTTATAAGAATGATGGAAAGT20.17513134851138354No Hit
CGACATACTCACGCGCGACACGAAACGAGAAAGCTCTTATCTCTTGTTCT20.17513134851138354No Hit
CGACATACTCACGCGCGACGCGTGATCAGTAGAAACAAGGGTGTTTTTTA20.17513134851138354No Hit
CGACATACTCACGCGCGACACGATGCAATTGTCATTCTAAGTGTCTCGCT20.17513134851138354No Hit
CGACATACTCACGCGCGACACGGGGGACAGAAATTTGAGGAAATAAGGTG20.17513134851138354No Hit
CGACATACTCACGCGCGACACGAGAATAGTTTCGAACAAATAACATTTAT20.17513134851138354No Hit
CGACATACTCACGCGCGACACGCGTGATCAGTAGAAACAAGGGTATTTTT20.17513134851138354No Hit
CGACATACTCACGCGCGACACGAAACAAGGGTATTTTTCCTCAACTGTCA20.17513134851138354No Hit
CGACATACTCACGCGCGACACGGGATCAGTAGAAACAAGGGTGTTTTTTA20.17513134851138354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCACG1050.095.000017
TCACGCG1050.095.000019
ACATACT1050.095.000013
CTCACGC1050.095.000018
TACTCAC1050.095.000016
CATACTC1050.095.000014
TGACATA154.983746E-495.01
GACATAC1100.090.6818162
ATACTCA1100.090.6818165
CGACATA950.090.000011
CGACACG900.047.50000416-17
ACACGGG204.2934847E-447.50000418-19
GCGACAC900.047.50000414-15
ACGCGCG1000.047.50000410-11
CGCGCGA950.047.50000412-13
CACGGGG204.2934847E-447.50000418-19
ACACGCC150.00901823847.518-19
ACACGCA150.00901823847.518-19
ACACGAA150.00901823847.518-19
GACACGG309.193918E-747.516-17
Produced by FastQC (version 0.11.5)