FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC313CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC313CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19422
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATAGTCGTACTCAGTGATAGAGGCCAAGGGTGTTGCCTCTTCCTTTTA900.46339202965708987No Hit
CGATAGTCGTACTCAGTGCTAGCCAGCACCATTCTGTTTTCATGCCTGAT890.45824322932756667No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAAATGGACGAAGGACAAGG780.4016064257028112No Hit
CGATAGTCGTACTCAGTGATAGCGGCCCAAGATCGAAGGTTCCAGGTTCC600.3089280197713933No Hit
CGATAGTCGTACTCAGTGATAGCGGCGCAAGATCGAAGGTTCCAGGTTCC590.30377921944187003No Hit
CGATAGTCGTACTCAGTGATAGAGGCCCATTAGAGCACATCCAGAAACTG580.29863041911234683No Hit
CGATAGTCGTACTCAGTGATAGCGGCCAAGGGTGTTGCCTCTTCCTTTTA560.2883328184533004No Hit
CGATAGTCGTACTCAGTGATAGCGCCGCAAGATCGAAGGTTCCAGGTTCC520.2677376171352075No Hit
CGATAGTCGTACTCAGTGATAGCGCCCCAAGATCGAAGGTTCCAGGTTCC500.25744001647616105No Hit
CGATAGTCGTACTCAGTGATAGCCCCCAGGGAGACTACCAGTACCAATGA450.23169601482854493No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAGGAGTTGAACCAAGATGC450.23169601482854493No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAGGCTCTATTGGGAAAGTG440.22654721449902174No Hit
CGATAGTCGTACTCAGTGATAGCGGCACAGATTGAAAGCGACAGAGAATA430.22139841416949851No Hit
CGATAGTCGTACTCAGTGATAGCCGCCCAAGATCGAAGGTTCCAGGTTCC420.2162496138399753No Hit
CGATAGTCGTACTCAGTGATAGAGACCCATTAGAGCACATCCAGAAACTG390.2008032128514056No Hit
CGATAGTCGTACTCAGTGATAGCGGCGCCATTCTGTTTTCATGCCTGATT340.17505921120378953No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCTATATGAAGCAATCGAGGAGTG330.1699104108742663No Hit
CGATAGTCGTACTCAGTGATAGCCGCGCAAGATCGAAGGTTCCAGGTTCC310.15961281021521986No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGCAATAGTTGGAGAAATTTCAC310.15961281021521986No Hit
CGATAGTCGTACTCAGTGATAGCGGCGCATTTCTTCAATTAACCACCTTA310.15961281021521986No Hit
CGATAGTCGTACTCAGTGATAGCGGCCCATTAGAGCACATCCAGAAACTG280.1441664092266502No Hit
CGATAGTCGTACTCAGTGATATCGAGGCCAAGGGTGTTGCCTCTTCCTTT280.1441664092266502No Hit
CGATAGTCGTACTCAGTGATAGCGGCGCAGATTGAAAGCGACAGAGAATA270.13901760889712697No Hit
CGATAGTCGTACTCAGTGATAGAGGCCCATGCAACTGGCAAGTGCACCAG270.13901760889712697No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGAAAATGGACGAAGGACAAGGG270.13901760889712697No Hit
CGATAGTCGTACTCAGTGATAGCGCCGCATTTCTTCAATTAACCACCTTA260.13386880856760375No Hit
CGATAGTCGTACTCAGTGATAGAGGCGCATTTCTTCAATTAACCACCTTA240.1235712079085573No Hit
CGATAGTCGTACTCAGTGATAGCCCCCAAGATCGAAGGTTCCAGGTTCCA240.1235712079085573No Hit
CGATAGTCGTACTCAGTGATAGCGGCGCCAAACTCTCCTTATTTCTTCTT240.1235712079085573No Hit
CGATAGTCGTACTCAGTGATAGCGGCACCATTCTGTTTTCATGCCTGATT230.11842240757903408No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGCAACATCTTCTCCTATTTCAT230.11842240757903408No Hit
CGATAGTCGTACTCAGTGATAGCGGCGCTTTTCATTTCTGCTCTGGAGGT220.11327360724951087No Hit
CGATAGTCGTACTCAGTGATAGCCCCGCAAGATCGAAGGTTCCAGGTTCC220.11327360724951087No Hit
CGATAGTCGTACTCAGTGATAGAGCCGCATTTCTTCAATTAACCACCTTA220.11327360724951087No Hit
CGATAGTCGTACTCAGTGATAGCCGCCAGGGAGACTACCAGTACCAATGA210.10812480691998765No Hit
CGATAGTCGTACTCAGTGATAGGGGGGAGGAAGGCTCTATTGGGAAAGTG200.10297600659046442No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGCAAAAACATAATGGACTCCAAC200.10297600659046442No Hit
CGATAGTCGTACTCAGTGATAGCGGCGCAAAATCTGTATTCAACAGTCTA200.10297600659046442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCA201.700782E-595.000019
AGGCGTA201.700782E-595.000015
CGTACCC201.700782E-595.000018
TCGTACC201.700782E-595.000017
TAGGCGT201.700782E-595.000014
CGATAGG156.5194326E-495.01
GTACTTA156.5194326E-495.09
GGCGTAC156.5194326E-495.06
CGTACTT156.5194326E-495.08
GCGTACT156.5194326E-495.07
CGTACTC18700.093.475948
TCGTACT18700.093.475947
GTACTCA18600.093.467749
AGTCGTA19350.091.563315
GTCGTAC19300.091.55446
CGATAGT19050.091.2598341
ATAGTCG19350.091.072353
TAGTCGT19500.090.1282044
GATAGTC19550.089.89772
TTGTCTT404.0454324E-947.50000494-95
Produced by FastQC (version 0.11.5)