FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC309CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC309CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11670
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGTATCTATAGTACTGCTAACGCGAAGTAGGTACAGATGCAATTGTC1991.7052270779777206No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTGTTT1881.6109682947729222No Hit
CGTAGTATCTATAGTACTGCTAAGCCCATACACACAAGCAGGCAGGCAGG1381.1825192802056554No Hit
CGTAGTATCTATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGAT1301.113967437874893No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTATTT950.8140531276778064No Hit
CGTAGTATCTATAGTACTGCTACCCCTCTTAGTTTGCATAGTTTCCCGTT740.6341045415595544No Hit
CGTAGTATCTATAGTACTGCTACCGCGAAGTAGGTACAGATGCAATTGTC730.6255355612682091No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGGGTTT590.5055698371893745No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGTCGTTT510.43701799485861187No Hit
CGTAGTATCTATAGTACTGCTAACGAGAAAGCTCTTATCTCTTGTTCTAC470.40274207369323056No Hit
CGTAGTATCTATAGTACTGCTAAAACGAGAAAGCTCTTATCTCTTGTTCT420.35989717223650386No Hit
CGTAGTATCTATAGTACTGCTAAATCCATGTTGTTCGGGTCCCCATTCCC390.33419023136246784No Hit
CGTAGTATCTATAGTACTGCTAAGGCGAAGTAGGTACAGATGCAATTGTC360.30848329048843187No Hit
CGTAGTATCTATAGTACTGCTACTCCCATTCGCTTCTGGTAGGCCTGCAA360.30848329048843187No Hit
CGTAGTATCTATAGTACTGCTACGCCCATACACACAAGCAGGCAGGCAGG350.29991431019708653No Hit
CGTAGTATCTATAGTACTGCTATGGGGCTACCCCTCTTAGTTTGCATAGT350.29991431019708653No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAAATGGACGAAGGACAAGG350.29991431019708653No Hit
CGTAGTATCTATAGTACTGCTAGAAACGAGAAAGCTCTTATCTCTTGTTC320.27420736932305056No Hit
CGTAGTATCTATAGTACTGCTAGAAACGGGAAAGCTCTTATCTCTTGTTC300.2570694087403599No Hit
CGTAGTATCTATAGTACTGCTACATCCATGTTGTTCGGGTCCCCATTCCC300.2570694087403599No Hit
CGTAGTATCTATAGTACTGCTACTGCAAGCCCATACACACAAGCAGGCAG280.23993144815766923No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAGTCTTCGAGCTCTCGGAC270.23136246786632392No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGGTGACAA260.22279348757497855No Hit
CGTAGTATCTATAGTACTGCTACGGCGAAGTAGGTACAGATGCAATTGTC250.21422450728363326No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGAAAATGGACGAAGGACAAGGGT240.2056555269922879No Hit
CGTAGTATCTATAGTACTGCTACGCGAAGTAGGTACAGATGCAATTGTCA230.19708654670094258No Hit
CGTAGTATCTATAGTACTGCTAAGGCCATACACACAAGCAGGCAGGCAGG220.18851756640959727No Hit
CGTAGTATCTATAGTACTGCTAATGCGAAGTAGGTACAGATGCAATTGTC220.18851756640959727No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGTAGTTT210.17994858611825193No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGCAAAAACATAATGGACTCCAAC200.17137960582690662No Hit
CGTAGTATCTATAGTACTGCTACCGCTCTTAGTTTGCATAGTTTCCCGTT190.16281062553556128No Hit
CGTAGTATCTATAGTACTGCTAACTCGAAGTAGGTACAGATGCAATTGTC190.16281062553556128No Hit
CGTAGTATCTATAGTACTGCTAAGTCCCGTTTTCGTTTCATTACCAACAC180.15424164524421594No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGACAAAAACATAATGGACTCCAA180.15424164524421594No Hit
CGTAGTATCTATAGTACTGCTACGTGTGATCAGTAGAAACAAGGGTGTTT180.15424164524421594No Hit
CGTAGTATCTATAGTACTGCTACTCCTCTTAGTTTGCATAGTTTCCCGTT170.1456726649528706No Hit
CGTAGTATCTATAGTACTGCTACTTCAAGCAGTAGTTGTAAGGCTTGCAT170.1456726649528706No Hit
CGTAGTATCTATAGTACTGCAAGCCCATACACACAAGCAGGCAGGCAGGA170.1456726649528706No Hit
CGTAGTATCTATAGTACTGCTAAAGCGAAGTAGGTACAGATGCAATTGTC170.1456726649528706No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGAGTTTT170.1456726649528706No Hit
CGTAGTATCTATAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACA160.13710368466152528No Hit
CGTAGTATCTATAGTACTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCA160.13710368466152528No Hit
CGTAGTATCTATAGTACTGCTAACCCTCTTAGTTTGCATAGTTTCCCGTT160.13710368466152528No Hit
CGTAGTATCTATAGTACTGCTACTGCGAAGTAGGTACAGATGCAATTGTC160.13710368466152528No Hit
CGTAGTATCTATAGTACTGCTAATGACCATCGTCAACATCCACAGCACTC160.13710368466152528No Hit
CGTAGTATCTATAGTACTGCTACGCCTCTTAGTTTGCATAGTTTCCCGTT160.13710368466152528No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGTAGATAT160.13710368466152528No Hit
CGTAGTATCTATAGTACTGCTACAGCCAGCAATGTTACATTTACCCAAAT150.12853470437017994No Hit
CGTAGTATCTATAGTACTGCTAAGTCCATACACACAAGCAGGCAGGCAGG150.12853470437017994No Hit
CGTAGTATCTATAGTACTGCTAGAAGGTTAACAGAGTGTGTTACTGTTAC140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTAGAGGGGGGAAAATGGACGAAGGACAAGG140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTACAAACGAGAAAGCTCTTATCTCTTGTTC140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTAGCGTGATCAGTAGAAACAAGGGTGTTTT140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTAACACGAAGTAGGTACAGATGCAATTGTC140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTACCCCACTTAGTTTGCATAGTTTCCCGTT140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTACGGCCAGCAATGTTACATTTACCCAAAT140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTACCTCCAGAGCAGAAATGAAAAGTGGCGA130.11139674378748927No Hit
CGTAGTATCTATAGTACTGCTAGCCCATACACACAAGCAGGCAGGCAGGA130.11139674378748927No Hit
CGTAGTATCTATAGTACTGCTACCCAAATGCAATGGGGCTACCCCTCTTA130.11139674378748927No Hit
CGTAGTATCTATAGTACTGCTACCCCGACCAGTGAGTACCCTTCCCTTTC130.11139674378748927No Hit
CGTAGTATCTATAGTACTGCTAGGGGACAGAAATTTGAGGAAATAAGGTG120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTAGGGGGACAAAAACATAATGGACTCCAAC120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTATTGGGACAGAAATTTGAGGAAATAAGGT120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTACCGCGCGTGATCAGTAGAAACAAGGGTG120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTAGGGGGACGAAAACATAATGGACTCCAAC120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTAAACGAGAAAGCTCTTATCTCTTGTTCTA120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTAGAACGAGAAAGCTCTTATCTCTTGTTCT120.10282776349614395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTATA11300.095.000017
TATCTAT11300.095.000016
CTATAGT11350.095.09
GTATCTA11350.095.05
CGTAGTA11550.095.01
TCTATAG11350.095.08
AGTATCT11450.095.04
GTAGTAT11600.094.5905152
TAGTATC11500.094.586953
CTACAGC205.2795716E-447.50000420-21
GCTAGGC205.2795716E-447.50000418-19
GCTAGAA452.055458E-1047.50000418-19
TAAGCCC205.2795716E-447.50000420-21
AGAGGAA205.2795716E-447.50000426-27
GCTACTC205.2795716E-447.50000418-19
GCTACGG452.055458E-1047.50000418-19
GCTACAG403.925379E-947.50000418-19
GCTACAC205.2795716E-447.50000418-19
GCTAACG800.047.50000418-19
GCTAAAA205.2795716E-447.50000418-19
Produced by FastQC (version 0.11.5)