FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC307CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC307CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7894
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATACAGAGTAGTACAGACCGGCGCTGGAGCTAGGATGAGTCCCAAT460.5827210539650367No Hit
CGACATACAGAGTAGTACACGCGTGATCAGTAGAAACAAGGGTGTTTTTT250.31669622498099825No Hit
CGACATACAGAGTAGTACAGACGCGTGATCAGTAGAAACAAGGGTGTTTT230.29136052698251835No Hit
CGACATACAGAGTAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGA220.2786926779832784No Hit
CGACATACAGAGTAGTACAGACCGGCACTGGAGCTAGGATGAGTCCCAAT200.25335697998479856No Hit
CGACATACAGAGTAGTACAGACCAGCGCTGGAGCTAGGATGAGTCCCAAT200.25335697998479856No Hit
CGACATACAGAGTAGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGG190.24068913098555864No Hit
CGACATACAGAGTAGTACAGACGCGTGATCAGTAGAAACAAGGGGGTTTT170.21535343298707882No Hit
CGACATACAGAGTAGTACAGACCGGCGCCAAACTCTCCTTATTTCTTCTT170.21535343298707882No Hit
CGACATACAGAGTAGTACAGACCGGCCAGGAAAATGAGGTCTTCAATCTC160.20268558398783887No Hit
CGACATACAGAGTAGTACAGACAGGAGCCCATATCGAACCCTAATGAGCT150.19001773498859895No Hit
CGACATACAGAGTAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCT150.19001773498859895No Hit
CGACATACAGAGTAGTACAGACCGGCACTGGGATGCTCTTCTAGGTATTT120.15201418799087915No Hit
CGACATACAGAGTAGTACAGACCAGCACTGGAGCTAGGATGAGTCCCAAT110.1393463389916392No Hit
CGACATACAGAGTAGTACAGACGGGGGGGGAACGCCAGGATGCCACAGAA110.1393463389916392No Hit
CGACATACAGAGTAGTACACGCGTGATCAGTAGAAACAAGGGGGTTTTTT100.12667848999239928No Hit
CGACATACAGAGTAGTACAGACAGTCCCATTTTTGACACTTTCCATGCAC100.12667848999239928No Hit
CGACATACAGAGTAGTACAGACACCCGCGGTCGATTCGAGCCATGCCAGT90.11401064099315936No Hit
CGACATACAGAGTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACT90.11401064099315936No Hit
CGACATACAGAGTAGTACAGAAAGCACCATCCTCTCTATTGTTATGCTAT90.11401064099315936No Hit
CGACATACAGAGTAGTACAGACCGGCCAGCAATGTTACATTTACCCAAAT90.11401064099315936No Hit
CGACATACAGAGTAGTAAAGACACTTTCCAGTCTCTGCGCGATCTCGGCT90.11401064099315936No Hit
CGACATACAGAGTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCTCTTATT90.11401064099315936No Hit
CGACATACAGAGTAGTACAGACCGCCCCTGGAAGGACAAATCTTCTGGCT90.11401064099315936No Hit
CGACATACAGAGTAGTACAGACATGCCCACTTGCTACTGCAAGCCCATAC80.10134279199391943No Hit
CGACATACAGAGTAGTACAAACACATCCCCCTTGGAACCGATTCTTATAC80.10134279199391943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTCA201.6377318E-595.000013
AGAGTAG7300.095.000019
TCAGAGT201.6377318E-595.000017
TTCAGAG201.6377318E-595.000016
ATTCAGA201.6377318E-595.000015
CGACATT201.6377318E-595.000011
TACAGAG7250.095.06
TGACATA156.373673E-495.01
AGAGGAG254.204776E-795.09
CAGAGTA7200.095.08
CAGAGGA254.204776E-795.08
ACATACA6950.095.03
CGTACAG156.373673E-495.04
CGACATA6900.095.01
ACAGAGT7050.094.999997
ATACAGA7200.094.340285
GACATAC7050.094.326232
CATACAG7050.094.326234
GACATTC301.2227267E-463.3333362
ACAGAGG406.8732916E-659.3750047
Produced by FastQC (version 0.11.5)