FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC295CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC295CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACGATACACGCGTATGTACGGCGCCATCAAGGGAGCAATTTTACAA562.7184466019417477No Hit
CGATACGATACACGCGTATGTACGCCGCCATCAAGGGAGCAATTTTACAA170.825242718446602No Hit
CGATACGATACACGCGTATGTACGGCCCCATCAAGGGAGCAATTTTACAA140.6796116504854369No Hit
CGATACGATACACGCGTATGTACGGCCAGCAATGTTACATTTACCCAAAT120.5825242718446602No Hit
CGATACGATACACGCGTATGTAAGGCGCCATCAAGGGAGCAATTTTACAA120.5825242718446602No Hit
CGATACGATACACGCGTATGTACGGCGCAAAGAGGGCCTATTATCTTTTG80.3883495145631068No Hit
CGATACGATACACGCGTATGTACGACGCCATCAAGGGAGCAATTTTACAA80.3883495145631068No Hit
CGATACGATACACGCGTATGTACGGCCAGGAACATTCGAGGATTTTGATT70.33980582524271846No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAAGAATACTGACATCAGAG70.33980582524271846No Hit
CGATACGATACACGCGTATGTAAGCCGCCATCAAGGGAGCAATTTTACAA70.33980582524271846No Hit
CGATACGATACACGCGTATGTACGGCGCTCCAGAGGGTTTGTCCTTGTTC70.33980582524271846No Hit
CGATACGATACACGCGTATGTACGCCACCATCAAGGGAGCAATTTTACAA60.2912621359223301No Hit
CGATACGATACACGCGTATGTACCGCGCCATCAAGGGAGCAATTTTACAA60.2912621359223301No Hit
CGATACGATACACGCGTATGTACGGCACCATCAAGGGAGCAATTTTACAA60.2912621359223301No Hit
CGATACGATACACGCGTATGTACGGCGCCAAATAGGCCTCTAGATTGAAT60.2912621359223301No Hit
CGATACGATACACGCGTATGTACGGCCCTTGGGTTAAGTGCAACACTTCA60.2912621359223301No Hit
CGATACGATACACGCGTATGTACGGCCCCAAATAGGCCTCTAGATTGAAT50.24271844660194172No Hit
CGATACGATACACGCGTATGTACGGCTCCATCAAGGGAGCAATTTTACAA50.24271844660194172No Hit
CGATACGATACACGCGTATGTAAGGCGCGAAGTAGGTACAGATGCAATTG50.24271844660194172No Hit
CGATACGATACACGCGTATGTAAGGCCCCAAATAGGCCTCTAGATTGAAT50.24271844660194172No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAAACAAAAGCAACAAAAAT50.24271844660194172No Hit
CGATACGATACACGCGTATGTACGGCGCCAAACTCTCCTTATTTCTTCTT40.1941747572815534No Hit
CGATACGATACACGCGTATGTAAGGCCAGCAATGTTACATTTACCCAAAT40.1941747572815534No Hit
CGATACGATACACGCGTATGTAAGGCGCCAAATAGGCCTCTAGATTGAAT40.1941747572815534No Hit
CGATACGATACACGCGTATGTACGCCCCCCTTCAATGAAACCGGCAATGG40.1941747572815534No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAAATGAGGTCTTCAATCTC40.1941747572815534No Hit
CGATACGATACACGCGTATGTACCGCGCAAAGAGGGCCTATTATCTTTTG40.1941747572815534No Hit
CGATACGATACACGCGTATGTACGGCGACCAAAACAATTCGACACTAATT40.1941747572815534No Hit
CGATACGATACACGCGTATGTACCCCGCTCCAGAGGGTTTGTCCTTGTTC40.1941747572815534No Hit
CGATACGATACACGCGTATGTACGGCTTGCATAAATGTTATTTGTTCGAA40.1941747572815534No Hit
CGATACGATACACGCGTATGTAAGGCGACCAAAACAATTCGACACTAATT40.1941747572815534No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAGAATACTGACATCAGAGT40.1941747572815534No Hit
CGATACGATACACGCGTATGTAAGGCACCATCAAGGGAGCAATTTTACAA40.1941747572815534No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAGAATACTGACATCAGAGT40.1941747572815534No Hit
CGATACGATACACGCGTATGTACTGCGCCATCAAGGGAGCAATTTTACAA40.1941747572815534No Hit
CGATACGATACACGCGTATGTACAGCGCCATCAAGGGAGCAATTTTACAA40.1941747572815534No Hit
CGATACGATACACGCGTATGTACGCCGCTCCAGAGGGTTTGTCCTTGTTC40.1941747572815534No Hit
CGATACGATACACGCGTATGTAGGGGGGGAAACAAAAGCAACAAAAATGA30.14563106796116504No Hit
CGATACGATACACGCGTATGTACGGACAGCAGACTCCACCCCAGATGTGC30.14563106796116504No Hit
CGATACGATACACGCGTATGTAAGGGGGGGAAAATGAGGTCTTCAATCTC30.14563106796116504No Hit
CGATACGATACACGCGTATGTAAGGCCATACACACAAGCAGGCAGGCAGG30.14563106796116504No Hit
CGATACGATACACGCGTATGTACCGCGCTCCAGAGGGTTTGTCCTTGTTC30.14563106796116504No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAACAAAATAACATTCGAAG30.14563106796116504No Hit
CGATACGATACACGCGTATGTACGGCCCCCTTCAATGAAACCGGCAATGG30.14563106796116504No Hit
CGATACGATACACGCGTATGTACGGCGAGCAATGTTACATTTACCCAAAT30.14563106796116504No Hit
CGATACGATACACGCGTATGTACGGCAATCGCACGCAAAGAGGGCCTATT30.14563106796116504No Hit
CGATACGATACACGCGTATGTACGGCGCCATCCGAATTCTTTTGGTCGCT30.14563106796116504No Hit
CGATACGATACACGCGTATGTACGTCCCTTGGGTTAAGTGCAACACTTCA30.14563106796116504No Hit
CGATACGATACACGCGTATGTACAGCGCAAAGAGGGCCTATTATCTTTTG30.14563106796116504No Hit
CGATACGATACACGCGTATGTACGCCCCCTTGCCCAATTAGCACATTAGC30.14563106796116504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC1800.095.000017
TACACGC1800.095.000019
ATACACG1800.095.000018
CGATACG1850.095.01
CGATACA1750.095.06
GATACGA1900.095.02
ATACGAT1750.095.03
ACGATAC1750.095.05
TACGATA1800.092.3611154
CTTTTGT301.1588108E-647.572-73
CTCTGAT301.1588108E-647.594-95
ACACGCG1900.047.510-11
GGGCAAG150.009612163547.526-27
CCAGCTG301.1588108E-647.584-85
GGCGCCA150.009612163547.524-25
CACGCGT1900.047.510-11
GGCAAGA150.009612163547.528-29
TATGTAG505.456968E-1247.516-17
TATGTAC1250.047.516-17
TTATTGC355.646143E-847.578-79
Produced by FastQC (version 0.11.5)