FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC292CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC292CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5896
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGAGTCTATATACGTCGAGGCCAAGGGTGTTGCCTCTTCCTTTTA220.3731343283582089No Hit
CGAGATGAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAAT200.33921302578018997No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGGAAAATGGACGAAGGACAAGG170.28833107191316143No Hit
CGAGATGAGTCTATATACGTCGCCACTCATTTATTCCTACGATATCTTGC130.22048846675712347No Hit
CGAGATGAGTCTATATACGTCGAGGCCCATGCAACTGGCAAGTGCACCAG120.20352781546811397No Hit
CGAGATGAGTCTATATACGTCAGAGGTGACAAGATTGGTCTTGTCTTTAG110.18656716417910446No Hit
CGAGATGAGTCTATATATGTCTGATTTACCCAAGTGTTGTTTTCATAAGT110.18656716417910446No Hit
CGAGATGAGTCTATATACGTCGCGGCGCATTTCTTCAATTAACCACCTTA110.18656716417910446No Hit
CGAGATGAGTCTATATACGTCCCCTTGCCCAATTAGCACATTAGCTTTCT100.16960651289009498No Hit
CGAGATGAGTCTATATACGTCGCGGCCAAGGGTGTTGCCTCTTCCTTTTA100.16960651289009498No Hit
CGAGATGAGTCTATATACGTCGCTGAACAAAACTCCCGCTATATCCTGAC100.16960651289009498No Hit
CGAGATGAGTCTATATACGTCGCGCCGCATTTCTTCAATTAACCACCTTA90.15264586160108548No Hit
CGAGATGAGTCTATATACGTCGCCAACATCTTTGCAGTCATCAAAGTCTA80.13568521031207598No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGAAAATGGACGAAGGACAAGGG70.11872455902306649No Hit
CGAGATGAGTCTATATACGTCGCGGCGCATGTACCATCTGCCTAGTCTGA70.11872455902306649No Hit
CGAGATGAGTCTATATACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCA70.11872455902306649No Hit
CGAGATGAGTCTATATACGTCGAGTCCCCATTCCCATTTAGGGCATTTTG70.11872455902306649No Hit
CGAGATGAGTCTATATACGTCGATTAGCAACCTCCATGGCCTCTGCTGCC70.11872455902306649No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAACATCTTCTCCTATTTCAT60.10176390773405698No Hit
CGAGATGAGTCTATATACGTCGCTGACCGTGCCAGGAAAATGAGGTCTTC60.10176390773405698No Hit
CGAGATGAGTCTATATACGTCGAGGCCCCATTCCCATTTAGGGCATTTTG60.10176390773405698No Hit
CGAGATGAGTCTATATACGACGCGTGATCAGTAGAAACAAGGGGGTTTTT60.10176390773405698No Hit
CGAGATGAGTCTATATACGTCGGAGGGGGGAAAATGGACGAAGGACAAGG60.10176390773405698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA4900.095.000017
TGAGTCT5000.095.000016
CGAGATG5150.095.01
GAGTTTA156.2908616E-494.999997
GTTTATA156.2908616E-494.999999
AGGCTAT156.2908616E-494.999998
GGCTATA201.6022634E-594.999999
TGAGGCT156.2908616E-494.999996
AGTTTAT156.2908616E-494.999998
GTCTATA5250.094.0952459
GAGATGA5150.094.077672
AGATGAG5100.094.068633
AGTCTAT5100.094.068638
ATGAGTC5200.091.346155
GATGAGT5200.090.432694
GATGAGG200.001972132671.254
ATGAGGC200.001972132671.255
GAGGCTA200.001972132671.257
TATACGT4950.047.50000414-15
TCGCGGC451.8735591E-1047.50000420-21
Produced by FastQC (version 0.11.5)