FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC263CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC263CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGAGCTTGAAAAGGAG90.48025613660619No Hit
CGTCGTACGATAGCACTAGTATGGGGGACGAGAGCGAGACTTCATCTCCA60.32017075773745995No Hit
CGTCGTACGATAGCACTAGTATCGCCGCCCCTGGAAGGACAAATCTTCTG50.26680896478121663No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGAACGAGAGAGAGAGA40.21344717182497333No Hit
CGTCGTACGATAGCACTAGTATCGGCCGGGGAAGAAGTGGACGTTTGAGT40.21344717182497333No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGTGGCACGATGAAACC40.21344717182497333No Hit
CGTCGTACGATAGCACTAGTATCAGCAAAAGCAGGGAGCTTGAAAAGGAG40.21344717182497333No Hit
CGTCGTACGATAGCACTAGTATCGGCTCTAGATTAAAGATTTAGATGCAG40.21344717182497333No Hit
CGTCGTACGATAGCACTAGTATCGGCCCCATGATCCAAACACCTCCCACC30.16008537886872998No Hit
CGTCGTACGATAGCACTAGTATGGGGGCCAAATAGGTTGACTAAGTCTTT30.16008537886872998No Hit
CGTCGTACGATAGCACTAGTATCGGCCCTAGATTAAAGATTTAGATGCAG30.16008537886872998No Hit
CGTCGTACGATAGCACTAGTATCTGCTCTAGATTAAAGATTTAGATGCAG30.16008537886872998No Hit
CGTCGTACGATAGCACTAGTATCGGCCCCCATGATCCAAACACCTCCCAC30.16008537886872998No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAAAGGAGGAGGAAGTAGAA30.16008537886872998No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGGGCTTGAAAAGGGG30.16008537886872998No Hit
CGTCGTACGATAGCACTAGTATCGGCCCCTGCTGTTCCAGGTTCTCTTCT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCCGCCCCCATGATCCAAACACCTCCCAC20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGAAAATAACATTCGAAGCAACT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGAAACGAGAAAGCTCTTATCTCTTGTTC20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGCCATCTATATTTGG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGCAAGTGTGAGTGAATGTGTGT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGTTTATTTATTTAGG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGCGCGGGAACAGATTGATAACGTTTAC20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCCGCCCCTGGAAGGACAAATCTTCTGGC20.10672358591248667No Hit
CGTCGTACGATAACACTAGTATGGGGGGGCAAATAGGTTGACTAAGTCTT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGGCGCCCCTGGAAGGACAAATCTTCTG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGCGGGGGAAAACAAAAGCAACAAAAATG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGCTGTTGACTGATACAGTTTGGGTATT20.10672358591248667No Hit
CATCGTACGATAGCACTAGTATCGGCCCCCATGATCCAAACACCTCCCAC20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGCAAGAAGGGGATTGAACTCAG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATAGGCAAAAGCAGGGAGCTTGAAAAGGAG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGGGATAAAAAGCTTC20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGCCGGGGAAGAAGTGGACGTTTGAGTG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAACCTGGAGGGGGAGAGATT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCCGCTCTAGATTAAAGATTTAGATGCAG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAGGGGATTGAACTCAGCAA20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATAGCCGCGAAGTAGGTACAGATGCAATTG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGCCGCTGAGCAGGAAGCCTGATGAGGG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGAGCTTGAAAAGGGG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCCGCGCCCATGATCCAAACACCTCCCCC20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGCGGGGAGAAGCTTTCAGTCTCGTACCA20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGCCTCCATGATCCAAACACCTCCCACC20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGCCCCTGGAAGGACAAATCTTCTGGCT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGCATAAGCAGACATCT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCCGCAAAAGCAGGGAGCTTGAAAAGGAG20.10672358591248667No Hit
CATCGTACGATAGCACTAGTATGGGGGGGGAAGTAGAAAAGAAAAGGGAG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATAGGCCCCCAAGATCGAAGGTTCCAGGTT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGAACCGCAATAAGAAACACAAAGCAAA20.10672358591248667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC1750.095.02
CGTACGA1700.095.04
CGTCGTA1750.095.01
TCGTACG1750.095.03
CGATAGC1600.094.999998
TACGATA1600.094.999996
ACGATAG1600.094.999997
GATAGCA1600.094.999999
GTACGAT1600.094.999995
GTATCGC252.3144594E-547.518-19
TAGCACT1650.047.510-11
AGTATCG650.047.518-19
AGTATCC150.00953661147.518-19
AGTATAG150.00953661147.518-19
TAGTATG600.047.516-17
TAGTATC950.047.516-17
AGCACTA1750.047.512-13
CTAGTAT1750.047.516-17
CACTAGT1700.047.514-15
ACTAGTA1750.047.514-15
Produced by FastQC (version 0.11.5)