FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC258CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC258CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26029
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGAGCAAGAGAG1500.5762803027392523No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGAGTTGAACT870.33424257558876636No Hit
CGTAGAGTATCTGAGTATACGCCGGCGCCAAACCTTATGTGAAATTGTAA780.2996657574244112No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGTGTTTTTT650.2497214645203427No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGCACTAAAG550.2113027776710592No Hit
CGTAGAGTATCTGAGTATACGCACACCCATTCACATGCACATTCACACAC510.19593530293134578No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGAAGCTTCA500.19209343424641748No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGGTTAGAAA500.19209343424641748No Hit
CGTAGAGTATCTGAGTATACGCCCGCGCCAAACCTTATGTGAAATTGTAA470.18056782819163242No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGTGAAAGATGT430.16520035345191902No Hit
CGTAGAGTATCTGAGTATACGCAGGCCCTAACACTGAGAATTTGCCTACC430.16520035345191902No Hit
CGTAGAGTATCTGAGTATACGCACACACAATACACACATATAAACGTATT340.13062353528756387No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGGTGTTTTC330.1267816666026355No Hit
CGTAGAGTATCTGAGTATACGCCCCCCAAAGCCAAAATAACCAGGAAAAA330.1267816666026355No Hit
CGTAGAGTATCTGAGTATACGCCTGCCCCAGGAAATACAAGCTAGGATGT300.11525606054785048No Hit
CGTAGAGTATCTGAGTATACGCGGGGGGGCAAGTGTGAGTGAATGTGTGT290.11141419186292212No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGAGATCTTCA290.11141419186292212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG201.7118262E-595.000011
TGTAGAG201.7118262E-595.000011
GTATCTG24700.095.07
AGTACCT156.5447803E-495.06
AGTATCT24700.095.06
AGAGTAC156.5447803E-495.04
GAGTACC156.5447803E-495.05
GTGGAGT156.5447803E-495.02
GTAGAGT24900.094.999992
ATCTGAG25050.094.810379
TAGAGTA24950.094.8096163
AGAGTAT24800.094.8084644
TATCTGA24800.094.8084648
GAGTATC24850.094.617715
CGTAGAG25250.094.435641
CATCTGA353.4544428E-881.4285668
GTAGAGC301.330005E-679.166672
TAGAGCA301.330005E-679.166673
TGGAGTA255.178938E-576.03
GTACCTG200.00205128171.257
Produced by FastQC (version 0.11.5)