FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC257CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC257CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATAGTCGTACGTATACATCCGGCCATCTGTTCCATAGCCTTTGCCGT240.5736137667304015No Hit
CGCATAGTCGTACGTATACATCCGGCGCCCAATTAGCACATTAGCTTTCT170.40630975143403447No Hit
CGCATAGTCGTACGTATACATCAGGCCAAACCTAACAAGGCTAGTCTTAC120.28680688336520077No Hit
CGCATAGTCGTACGTATACATCAGTAGCAGTGTTCTCTCTTTTATTCCTG120.28680688336520077No Hit
CGCATAGTCGTACGTATACATCCGCCGCCCAATTAGCACATTAGCTTTCT110.26290630975143403No Hit
CGCATAGTCGTACGTATACATCAGGCGCCCAATTAGCACATTAGCTTTCT90.21510516252390058No Hit
CGCATAGTCGTACGTATACATCGGGGGGGGAACAGTGACCACAGAAGCTG90.21510516252390058No Hit
CGCATAGTCGTACGTATACATCCGGCCAAACCTAACAAGGCTAGTCTTAC80.19120458891013384No Hit
CGCATAGTCGTACGTATACATCAGCAGATAAGAATTGAAAGTGAAAGTAA70.16730401529636713No Hit
CGCATAGTCGTACGTATACATCAGTAGAAACAAGGCCGTTTTTAAACAAT60.14340344168260039No Hit
CGCATAGTCGTACGTATACATCAGTAGCAGTGTTCTCTCTTTTTTTCCTG50.11950286806883366No Hit
CGCATAGTCGTACGTATACATCCGGCGCAAAATGCCATAAGCACCACATT50.11950286806883366No Hit
CGCATAGTCGTACGTATACATCCGGCCCCTTGCCCAATTAGCACATTAGC50.11950286806883366No Hit
CGCATAGTCGTACGTATACATCGGGGGGGGAAAGGAAATGTAATGCATTT50.11950286806883366No Hit
CGCATAGTCGTACGTATACATCCAGCCATCTGTTCCATAGCCTTTGCCGT50.11950286806883366No Hit
CGCATAGTCGTACGTATACATCAGGCGCAAAATGCCATAAGCACCACATT50.11950286806883366No Hit
CGCATAGTCGTACGTATACATCCGGCCCAACCTAACAAGGCTAGTCTTAC50.11950286806883366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC3750.095.07
TAGTCGT3750.095.05
ATAGTCG3950.095.04
AGTCGTA3750.095.06
CGTACGT3750.095.09
CATAGTC3850.095.03
TCGTACG3750.095.08
CGCATAG3800.095.01
GCATAGT3800.095.02
TACATCC2350.047.50000416-17
ATCCCCC150.00997044647.520-21
CGTCAAA150.00997044647.564-65
TTCACGT150.00997044647.560-61
GGGCAAA150.00997044647.526-27
CGGGGGG301.3199788E-647.522-23
CTGGGCC150.00997044647.590-91
AATCAAT150.00997044647.572-73
CACGTCA150.00997044647.562-63
GGTCCAT150.00997044647.592-93
CATCCGT150.00997044647.518-19
Produced by FastQC (version 0.11.5)