FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC248CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC248CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10470
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACAGCTACTACTACTCTAATTCGCTTCTGGTAGGCCTGCAAATTTT780.7449856733524355No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTCGTTT690.6590257879656161No Hit
CGATACAGCTACTACTACTCTAACGCGAAGTAGGTACAGATGCAATTGTC640.6112702960840497No Hit
CGATACAGCTACTACTACTCTACGTCCCCTTGCCCAATTAGCACATTAGC610.5826170009551098No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCCGTTT510.4871060171919771No Hit
CGATACAGCTACTACTACTCTACGGCCCCTTGCCCAATTAGCACATTAGC450.42979942693409745No Hit
CGATACAGCTACTACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCAT430.4106972301814709No Hit
CGATACAGCTACTACTACTCTACGTCGCCTTGCCCAATTAGCACATTAGC410.3915950334288443No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGGTGTTT300.28653295128939826No Hit
CGATACAGCTACTACTACTCTAGAAACGAGAAAGCTCTTATCTCTTGTTC250.2387774594078319No Hit
CGATACAGCTACTACTACTCTACGACCCCTTGCCCAATTAGCACATTAGC230.21967526265520534No Hit
CGATACAGCTACTACTACTCTACGGCAAAGGCTATGGAACAGATGGCTGG200.19102196752626552No Hit
CGATACAGCTACTACTACTCTACGCCCCCTTGCCCAATTAGCACATTAGC190.18147086914995225No Hit
CGATACAGCTACTACTACTCTAAGTCCCCTTGCCCAATTAGCACATTAGC180.17191977077363896No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCGATCT180.17191977077363896No Hit
CGATACAGCTACTACTACTCCAACACCATGTCAAGCTTTCAGGTAGACTG170.1623686723973257No Hit
CGATACAGCTACTACTACTCTACAGAGCAGAAATGAAAAGTGGCGAGAGC170.1623686723973257No Hit
CGATACAGCTACTACTACTCTACGGAGCAGAAATGAAAAGTGGCGAGAGC160.15281757402101243No Hit
CGATACAGCTACTACTACACTACGTCCCCTTGCCCAATTAGCACATTAGC160.15281757402101243No Hit
CGATACAGCTACTACTACTCTAGAAACGGGAAAGCTCTTATCTCTTGTTC150.14326647564469913No Hit
CGATACAGCTACTACTACTCTATGTTGACAAAATGACCATCGTCAACATC150.14326647564469913No Hit
CGATACAGCTACTACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCA150.14326647564469913No Hit
CGATACAGCTACTACTACTCTACCTCCAGAGCAGAAATGAAAAGTGGCGA150.14326647564469913No Hit
CGATACAGCTACTACTACTCTACGGCCAGAGGTGATACCATCACTCGGTC150.14326647564469913No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTGATCT140.13371537726838587No Hit
CGATACAGCTACTACTACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTT140.13371537726838587No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGGGGTTT140.13371537726838587No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTACTTT140.13371537726838587No Hit
CGATACAGCTACTACTACTCTACTAACCATTAATCTTGCGTAGCAGAGGC130.12416427889207259No Hit
CGATACAGCTACTACTACACTACTAACCATTAATCTTGCGTAGCAGAGGC130.12416427889207259No Hit
CGATACAGCTACTACTACTCTATTGGGAAAGTGTGCAGGACCTTACTGGC130.12416427889207259No Hit
CGATACAGCTACTACTACTCTACGGCGCCTTGCCCAATTAGCACATTAGC130.12416427889207259No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCGTTTT130.12416427889207259No Hit
CGATACAGCTACTACTACTCTAACACCATGTCAAGCTTTCAGGTAGACTG120.1146131805157593No Hit
CGATACAGCTACTACTACTCTACTGAGCAGAAATGAAAAGTGGCGAGAGC120.1146131805157593No Hit
CGATACAGCTACTACTACTCTAACGCCATGTCAAGCTTTCAGGTAGACTG120.1146131805157593No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTAGTTT120.1146131805157593No Hit
CGATACAGCTACTACTACTCTACCGCGAAGTAGGTACAGATGCAATTGTC110.10506208213944604No Hit
CGATACAGCTACTACTACTCTACGTCTCCTTGCCCAATTAGCACATTAGC110.10506208213944604No Hit
CGATACAGCTACTACTACTCTACCCACAACCGGTAACAACAAGCCCAGGA110.10506208213944604No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCCTGAG110.10506208213944604No Hit
CGATACAGCTACTACTACTCTAGAGTCCCGTTTTCGTTTCATTACCAACA110.10506208213944604No Hit
CGATACAGCTACTACTACTCTAAAACGAGAAAGCTCTTATCTCTTGTTCT110.10506208213944604No Hit
CGATACAGCTACTACTACTCTACTTCAAGCAGTAGTTGTAAGGCTTGCAT110.10506208213944604No Hit
CGATACAGCTACTACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTC110.10506208213944604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACTA9800.095.000018
TACAGGT156.434349E-495.04
GATACAG10050.095.02
AGCTACT9950.095.07
GCTACTT156.434349E-495.08
AGGTACT156.434349E-495.07
GGTACTA156.434349E-495.08
ATACAGG156.434349E-495.03
ATACAGC9950.095.03
CAGCTAC9900.094.999996
CGATACA10050.094.527371
ACAGCTA9950.094.522615
TACAGCT9850.094.517774
GTACTAC200.002016861571.259
ACAGGTA200.002016861571.255
CAGGTAC250.004883528757.0000046
TTATGAC205.26694E-447.50000472-73
ACTAACC205.26694E-447.50000422-23
TCTAGGA403.8926373E-947.50000418-19
TCTAGAA205.26694E-447.50000418-19
Produced by FastQC (version 0.11.5)