FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC216CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC216CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20892
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTGTGATAGTATCTCGTAGCTGAAACGAAAACGTTCTAATCTCTTGT1030.4930116791116217No Hit
CGAGTGTGATAGTATCTCGTAGAAACAAGGTAGTTTTTTACTCTAGCTCT940.4499329887038101No Hit
CGAGTGTGATAGTATCTCGTAGAGAGGATTCTTCTTTCAGAATCCTCTCT870.4164273406088455No Hit
CGAGTGTGATAGTATCTCGTAGAGTCCCGTTTTCGTTTCATTACCAACAC720.34462952326249285No Hit
CGAGTGTGATAGTATCTCGTAGCCGCAAGTATAATTGGGATTGTGCACTT680.3254834386367988No Hit
CGAGTGTGATAGTATCTCGTAGCAGCCCTCTGACTAACTCAACCCAGAAG680.3254834386367988No Hit
CGAGTGTGATAGTATCTTCTAGGGAGAGTTGAACCTTGCATCAAAGAGCA650.31112387516752826No Hit
CGAGTGTGATAGTATCTCGTAGAAACAAGGTCGTTTTTAAATTATTCGAA590.2824047482289872No Hit
CGAGTGTGATAGTATCTCGTAGTTTTTTACTCTAGCTCTATGCTGACAAA550.26325866360329314No Hit
CGAGTGTGATAGTATCTCGTAGCGGCAACGATTCAAGTGATCCTCTTGTT530.2536856212904461No Hit
CGAGTGTGATAGTATCTCGTAGATAAGCTGAAACGAAAACGTTCTAATCT490.23453953666475205No Hit
CGAGTGTGATAGTATCTCGTAGAAAGGGGTTTGAGATGATTTGGGATCCT440.2106069308826345No Hit
CGAGTGTGATAGTATCTCGTAGAAACAAGGCAGTTTTTTACTCTAGCTCT440.2106069308826345No Hit
CGAGTGTGATAGTATCTCGTAGCCACAAAAAGAAATGCTGCTCCCACTAG430.205820409726211No Hit
CGAGTGTGATAGTATCTCGTAGCGCCACAAAAAGAAATGCTGCTCCCACT430.205820409726211No Hit
CGAGTGTGATAGTATCTCGTAGTGGACCATCAATTGGGTTAAGTTGCGGT410.19624736741336396No Hit
CGAGTGTGATAGTATCTCGTAGGGGGGGCAAATGCAACGATTCAAGTGAT390.18667432510051696No Hit
CGAGTGTGATAGTATCTCGTAGAAGCAAGGTAGTTTTTTACTCTAGCTCT380.18188780394409343No Hit
CGAGTGTGATAGTATCTCGTAGCAGCCATCATCACCGAGGAGCAAGGCTG370.17710128278766993No Hit
CGAGTGTGATAGTATCTCGTAGACGCCACAAAAAGAAATGCTGCTCCCAC370.17710128278766993No Hit
CGAGTGTGATAGTATCTCGTAGAAACAAGGGTGTTTTTTATCACTAAATA360.17231476163124643No Hit
CGAGTGTGATAGTATCTCGTAGTTGTTGCCGCAAGTATAATTGGGATTGT350.1675282404748229No Hit
CGAGTGTGATAGTATCTCGTAGACTCCCATTGATTCCAATTTCACTCCAT340.1627417193183994No Hit
CGAGTGTGATAGTATCTCGTAGGAAGGGGCAAAAGTAACAGACTTAGAAA330.15795519816197587No Hit
CGAGTGTGATAGTATCTCGTAGAAACGAGGTAGTTTTTTACTCTAGCTCT320.15316867700555237No Hit
CGAGTGTGATAGTATCTCGTAGAAGCAAGGTCGTTTTTAAATTATTCGAA320.15316867700555237No Hit
CGAGTGTGATAGTATCTCGTAGTTGCCGCAAGTATAATTGGGATTGTGCA310.14838215584912887No Hit
CGAGTGTGATAGTATCTCGTAGTTTCGGTATTTTTTGTCCATCTTCCTCT310.14838215584912887No Hit
CGAGTGTGATAGTATCTCGTAGGTTATGGTGAAAGAAATTTCAGTGTCCT300.14359563469270534No Hit
CGAGTGTGATAGTATCTCGTAGGAAGGACCAAAAGTAACAGACTTAGAAA300.14359563469270534No Hit
CGAGTGTGATAGTATCTCTTATGACAAAAACATCTCCTTTGGAGCCAATT300.14359563469270534No Hit
CGAGTGTGATAGTATCTCGTAGGGGGTGCAGATGCAACGATTCAAGTGAT290.13880911353628184No Hit
CGAGTGTGATAGTATCTCGTAGAAACCAGGTAGTTTTTTACTCTAGCTCT290.13880911353628184No Hit
CGAGTGTGATAGTATCTCGTAGATTTTTGGATAGTGAATAGTACTTGCCA280.1340225923798583No Hit
CGAGTGTGATAGTATCTCGTAGTTATGGTGAAAGAAATTTCAGTGTCCTG280.1340225923798583No Hit
CGAGTGTGATAGTATCTCGTAGGGGGCCGCAAGTATAATTGGGATTGTGC270.1292360712234348No Hit
CGAGTGTGATAGTATCTCGTAGGTTGCATATTCCAGTGTTGTTTTGACTT260.1244495500670113No Hit
CGAGTGTGATAGTATCTCGTAGATGCAACGATTCAAGTGATCCTCTTGTT260.1244495500670113No Hit
CGAGTGTGATAGTATCTCGTAGAAGGCGGGAACAACTAGGTCAGAAGTTT250.11966302891058779No Hit
CGAGTGTGATAGTATCTAATAGCGCCAAGGTATGCTTTCGCACTGAGTAG250.11966302891058779No Hit
CGAGTGTGATAGTATCTCGTAGCCCATCCACTGATAGAACAAAGAGATGA230.11008998659774076No Hit
CGAGTGTGATAGTATCTCGTAGGTGCTGTTTTCATATGTGATGATTCTTT220.10530346544131725No Hit
CGAGTGTGATAGTATCTCGTAGATCAGCAAAAGCAGGTAGATATTGAAAG220.10530346544131725No Hit
CGAGTGTGATAGTATCTCGTAGGAAGGGCCAAAAGTAACAGACTTAGAAA220.10530346544131725No Hit
CGAGTGTGATAGTATCTCGTAGGGGGGTGCAGATGCAACGATTCAAGTGA220.10530346544131725No Hit
CGAGTGTGATAGTATCTCGTAGAGACAAGGAAGTTTTTTACTCTAGCTCT210.10051694428489374No Hit
CGAGTGTGATAGTATCTCGTAGAAGGCCCTCTTTTTAAACCGTGTTTAAA210.10051694428489374No Hit
CGAGTGTGATAGTATCTCGTAGAAACAAGGCCGTTTTTAAATTATTCGAA210.10051694428489374No Hit
CGAGTGTGATAGTATCTCGTAGGGGGCGGGAACAACTAGGTCAGAAGTTT210.10051694428489374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATAGT19650.095.000017
CGAGTGT19650.095.000011
GAGTGGG156.526371E-495.02
GTGGGAT156.526371E-495.04
AGTGTGA19700.095.03
CGAGTGG156.526371E-495.01
GTGTGAT19550.095.04
TGTGATA19550.095.05
AGGGTGA201.703803E-594.999993
CGAGGGT201.703803E-594.999991
CGAGGGG201.703803E-594.999991
GGGATAG353.092282E-1094.999996
GTGATAG19750.094.75956
GAGTGTG19900.094.522612
GATAGTA20200.094.05948
ATAGTAT20150.094.057079
GAGGGTG255.15477E-576.02
GGGGATA255.15477E-576.05
AGTGGGA200.002045543971.253
GGATAGT408.632196E-871.257
Produced by FastQC (version 0.11.5)