FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC193CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC193CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259955
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGTATAGACGTGATACTGAAACGAGAAAGCTCTTATCTCTTGTTCT36771.4144755823123234No Hit
CGTATGTATAGACGTGATACTGAAGAGCTTCCCAGCACTGGGATGCTCTT31661.2179030986132215No Hit
CGTATGTATAGACGTGATACTGTGTCCATGGACACAGTAAACAGAACACA30321.1663557154122828No Hit
CGTATGTATAGACGTGATACTGGCGGGGAGCGCCAGGATGCCACAGAAAT29741.1440441614894885No Hit
CGTATGTATAGACGTGATACTGTGTCCATGGAACAGGAACAGGATACACC21560.8293743147852513No Hit
CGTATGTATAGACGTGATACTGGACCACAACTGCCTGTCTTATCATTAGG18990.7305110499894212No Hit
CGTATGTATAGACGTGATACTGATGCAATTGTCGTTCTAAGTGTCTCGCT15390.5920255428824219No Hit
CGTATGTATAGACGTGATACTGTTTTCTTAGGGTCCTTCCCAGCACTGGG13460.5177819237945029No Hit
CGTATGTATAGACGTGATACTGAAAAGGGAAAGATAGTCAAATCAGTCGA11260.4331518916735589No Hit
CGTATGTATAGACGTGATACTGAAGATCTTGTATGAGGCCTGTCCATCAC10110.38891346579215635No Hit
CGTATGTATAGACGTGATACTGCAAGCCCATACACACAAGCAGGCAGGCA9080.3492912234809871No Hit
CGTATGTATAGACGTGATACTGAAACAAGGCATTTTTTCATGAAGGACAA8440.3246715777730761No Hit
CGTATGTATAGACGTGATACTGAACAAAACTCCCGCTATATCCTGACCAC7660.29466638456655964No Hit
CGTATGTATAGACGTGATACTGAGAAACAAGGCATTTTTTCATGAAGGAC7220.2777403781423708No Hit
CGTATGTATAGACGTGATACTGAGGCCTATTCAACTGGTGCACTTGCCAG7100.2731241945721375No Hit
CGTATGTATAGACGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTAC6890.2650458733242292No Hit
CGTATGTATAGACGTGATACTGTTTACACCACAAAAGGATATGCTGCTCC6820.2623530995749264No Hit
CGTATGTATAGACGTGATACTGATCCCCTCAGAATGAGTGCTGACCGTGC6700.2577369160046931No Hit
CGTATGTATAGACGTGATACTGTTACATTCTTTTCTAGTACTGTGTCTAC6510.25042795868515705No Hit
CGTATGTATAGACGTGATACTGTTTAAGGTCTCCAACATCTTTGCAGTCA6450.2481198669000404No Hit
CGTATGTATAGACGTGATACTGAATAGCATAACAATAGAAAGCACCATCC6350.24427304725817928No Hit
CGTATGTATAGACGTGATACTGATTAGCAACCTCCATGGCCTCTGCTGCC6000.2308091785116655No Hit
CGTATGTATAGACGTGATACTGGACGGATCATGATCTCAGAGAACTCTTC5890.22657767690561825No Hit
CGTATGTATAGACGTGATACTGAAGCGAATGGGAGTGCAGATGCAGCGAT5850.22503894904887384No Hit
CGTATGTATAGACGTGATACTGGTGGGGAGCGCCAGGATGCCACAGAAAT5470.2104210344098017No Hit
CGTATGTATAGACGTGATACTGAGCTCAATTGCTCTCTTAGCTCCTCATA5390.2073435786963128No Hit
CGTATGTATAGACGTGATACTGAAATGGGAGGCTGGTGTTTATAGCACCC5380.20695889673212672No Hit
CGTATGTATAGACGTGATACTGCTGAGCTTTGGGTATGAATTTCCTTTTT5350.2058048508395684No Hit
CGTATGTATAGACGTGATACTGGGAGGCTGGTGTTTATAGCACCCTTGGG4890.18810948048700737No Hit
CGTATGTATAGACGTGATTTTGATTCCCAAGTTGAATTGAGTGGCTAATC4880.18772479852282126No Hit
CGTATGTATAGACGTGATACTGATGGACGACTAAAGCAGAAAGCACCATC4750.18272393298840184No Hit
CGTATGTATAGACGTGATACTGAAGAGCACACAGAATGCCATTGACGAGA4380.1684907003135158No Hit
CGTATGTATAGACGTGATACTGAAGAAACAAGGCATTTTTTCATGAAGGA4370.1681060183493297No Hit
CGTATGTATAGACGTGATACTGGAGGGGTGAAAATGGACGAAGGACAAGG4300.16541324460002693No Hit
CGTATGTATAGACGTGATACTGTGAGGAATGCTCCAGTTATCCCTGCACA4220.16233578888653805No Hit
CGTATGTATAGACGTGATACTGAAACAAGGTATTTTTTCATGAAGGACAA4130.15887365120886307No Hit
CGTATGTATAGACGTGATACTGATTTGACTATCTTTCCCTTTTCTATTCT4030.15502683156700198No Hit
CGTATGTATAGACGTGATACTGTGAATCAGCAATCTGTTCACAAGTGGCA3940.151564693889327No Hit
CGTATGTATAGACGTGATACTGGTTGAAAGACACCCACGGTCGATTCGAG3930.15118001192514088No Hit
CGTATGTATAGACGTGATACTGGAGGGGGGAAAATGGACGAAGGACAAGG3870.14887192014002423No Hit
CGTATGTATAGACGTGATACTGTGATGAGAATGGGAGACCTTCACTACCT3850.14810255621165203No Hit
CGTATGTATAGACGTGATACTGCAGCGATTCAAGTGATCCTCTCGTCATT3830.14733319228327982No Hit
CGTATGTATAGACGTGATACTGGAAAAGGGAAAGATAGTCAAATCAGTCG3800.14617914639072146No Hit
CGTATGTATAGACGTGATACTGGGGGGGGGAAAATGGACGAAGGACAAGG3730.1434863726414187No Hit
CGTATGTATAGACGTGATACTGATTCCCAAGTTGAATTGAGTGGCTAATC3670.14117828085630205No Hit
CGTATGTATAGACGTGATACTGATCTTCAGAATAGAAAAGGGAAAGATAG3630.13963955299955763No Hit
CGTATGTATAGACGTGATACTGACCATCGTCAACATCCACAGCACTCTGC3550.13656209728606875No Hit
CGTATGTATAGACGTGATACTGATTGCCCCCAGGGAGACTACCAGTACCA3520.13540805139351042No Hit
CGTATGTATAGACGTGATACTGAGAAACAAGGTATTTTTTCATGAAGGAC3520.13540805139351042No Hit
CGTATGTATAGACGTGATACTGTTTGCTGTGTCCATGGAACAGGAACAGG3390.130407185859091No Hit
CGTATGTATAGACGTGATACTGAAGGACAAGTTAAATTCATTATTTTTGC3330.12809909407397432No Hit
CGTATGTATAGACGTGATACTGAATAGCATAACAATAGAGAGGATGCTCT3290.1265603662172299No Hit
CGTATGTATAGACGTGATACTGAAAAGGGGAAGATAGTCAAATCAGTCGA3240.12463695639629938No Hit
CGTATGTATAGACGTGATACTGCCCCGGACAATGGGGCAGTGGCTGTGTT3200.12309822853955492No Hit
CGTATGTATAGACGTGATACTGTTGTGGTCCAGTATCGTCTAATGGAGCA3040.11694331711257719No Hit
CGTATGTATAGACGTGATACTGAACACACCAATACTCAGAAAAGAACAGA3020.11617395318420495No Hit
CGTATGTATAGACGTGATACTGGGGGGGAGCGCCAGGATGCCACAGAAAT2980.11463522532746052No Hit
CGTATGTATAGACGTGATACTGAGGAGCTAAGAGAGCAATTGAGCTCAGT2950.1134811794349022No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGAAAGCACCATCCTCTCTATT2900.11155776961397165No Hit
CGTATGTATAGACGTGATACTGGCGAGAGCAATTGGGACAGAAATTTGAG2870.11040372372141333No Hit
CGTATGTATAGACGTGATACTGAAACGAGGAAGCTCTTATCTCTTGTTCT2860.11001904175722721No Hit
CGTATGTATAGACGTGATACTGGAACCGGGAACCCTCGATTGCTTCATAT2850.10963435979304109No Hit
CGTATGTATAGACGTGATACTGCCCCAGACAATGGGGCAGTGGCTGTGTT2840.109249677828855No Hit
CGTATGTATAGACGTGATACTGTTGTCTTTACTGTATATAGCCCATCCAC2820.10848031390048277No Hit
CGTATGTATAGACGTGATACTGCAGCCCATACACACAAGCAGGCAGGCAG2800.10771094997211056No Hit
CGTATGTATAGACGTGATACTGAGCAAGATTGCTCAGTTCATTGATGCTT2750.10578754015118001No Hit
CGTATGTATAGACGTGATACTGTTCACCACTGTCCAGGCCATGATTCGGT2740.1054028581869939No Hit
CGTATGTATAGACGTGATACTGTGAGGAATGCTCCTGTTATCCTGATTCT2690.10347944836606336No Hit
CGTATGTATAGACGTGATACTGGCGGGGGGCGCCAGGATGCCACAGAAAT2650.10194072050931892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAC301.22818165E-895.07
AGGTATA450.095.04
CGTATGC301.22818165E-895.01
TGGACGT353.2923708E-1095.09
GTTTAGA301.22818165E-895.06
CTATAGA156.612117E-495.06
GTCTAGA156.612117E-495.06
TCTAGAC156.612117E-495.07
AGTATAG156.612117E-495.05
GAGACGT450.095.09
CGTAGGT500.095.01
CGTGTGT301.22818165E-895.01
TATAGTC156.612117E-495.07
TATAGGC500.095.07
ACGTATA254.610538E-795.04
ATAGTCG156.612117E-495.08
GTACGTA254.610538E-795.02
CTATGTA201.7412814E-594.999992
ATGTATG409.094947E-1294.999994
CGTAAGT201.7412814E-594.999991
Produced by FastQC (version 0.11.5)