FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC179CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC179CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGACGTACAGCGCGTGTAGAGAACGCAAGAGTCTGAATGTGCATAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGTGTCTCGCTGGATTCCTCTTTCAAGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAACAGACGGAAATAGCAATAAGACCCAAA10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTACCCTTCCCTTTCAAAGTCATGCCCACTT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAAGGATAGAGTTCTCAATCGGCTGAAGAG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGTTGTGCTCCATTAGACGATACTGGACC10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTACGGCAATGGTGTTTGGATAGGGAGAACT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGAAAGAGAAGAACCCCCCCCCCCAAAA10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTACTGCAACATCTTCTCCTATTTCATCAAG10.7299270072992701No Hit
CTCGTGACGTACAGCGCGTGTAAATGGTTCTTGCTTTACTGTAATGACCG10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAGACCAAAAGCAGCTTCTGTGGTCACTGT10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTACAGGGATGGTAGATGGATGGTACGGTTA10.7299270072992701No Hit
CGCGGGACGTACAGCGCGGGTACAGATGCAATTGTCGGTCTCAGGGTCTC10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAGGTACAGATGCAATTGTCGTTCTAAGTG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGAGTGCAGATGCAGCGATTCAAGTGAT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAACGGAGCAAATGGAGTAAAAGGATTTTC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAATGGTTCTTGCTTTACTGTAATGACCG10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAAGGAGAGGAATTGCCCGCTAATTTCACG10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGGTACCGATGCAATTGTCGTTCTAAGGG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGTAGAAACAAGGTAGTTTTTTACTCTAG10.7299270072992701No Hit
CGCATGACGTACAGCGCGTGTAGTGCTAAGTGTCTCGCTGGATTCCTCTT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACCGAGATATGCATTCTGTGTGCTCTTCA10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTACTGCAAAAGCAGGTACTGATCCAAAATG10.7299270072992701No Hit
CGCCTGACGTACAGCGCGTGTACGAAACACAATGGACTCCAACACCATGT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTATACGTCTGATTTACCCAAGTGTTGTTTTCA10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAATAACCGACACTATCAAGAGTTGGAGAA10.7299270072992701No Hit
CGGCTGACGTACAGCGCGTGTAAGAAGAGCTCCAGGAGTGTACATCTGCT10.7299270072992701No Hit
CGCCTGACGTACAGCGCGTGTACAGGGGCTACCCCTCTTAGTTTGCATAG10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGATTCTGAAATGGGAGGCTGGGGTTTAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTATGCTAGAGTCCCGTTTTCGTTTCATTAC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGCACCCCCAAAATCTGTATTCAACAGTC10.7299270072992701No Hit
CGCATGACGTACAGCGCGTGTAGAGAGGACAAGTTAAATTCATTATTTTT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGAAGAAGGAAGAGCTCCAGGAGTGTACA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGAGGCAAAAGCAGGTCAAATATATTC10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTATGTCTGATTTACCCAAGTGTTGTTTTCA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAAGGAGAGAAAACTAATGTGCTAAGTAT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTATTTTGGACATTTTCCAATTGTGATCGGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACAGCGAGACACTTAGGACCCCAACTGCA10.7299270072992701No Hit
CGCGTGAAGGACAGCGCGTGTAAGTAGAAACAAGGTCGTTTTTAAACAAT10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTATGAGGCCTTTCCATCACTTGGTCCATCG10.7299270072992701No Hit
CGCGTGACGTACAGCCCGTGTTGCAAATGTATATAGCAGAACTACTAGTA10.7299270072992701No Hit
CTCGTGACGTACAGCGCGTGTATGAGGCCTGTCCATCACTTGGTCCATCG10.7299270072992701No Hit
CGCGGGACGTACAGCGCGTGGAAACAGAATGGGGCTGGCTAGCACTACGG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTATGAACCCATACAGGTTTGTTTTCCGTCT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTATCTTTCTGACATGACCGTCGAGGAAATG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACCGAGATATGCATTTCTTTCCATTGAGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACTGGGTTTCGACTTTCTCTTACTTGATC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGAAAGGGAAAGATAGTCAAATCAGGAT10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAGACGAAACAAGGTTATTTTTCCTCAGCT10.7299270072992701No Hit
CGCATGACGTACAGCGCGTGTATTTCAACCAGATTCTGGTTGAAAGACAC10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAAGTAGAGGGTTTGTCCTTGTTCATTCCT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTACGGCACGCACTCGAACATGCTACGTCCT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGCATGAACCAGTCTCGCGACATTTCCTC10.7299270072992701No Hit
CCCGTGACGTACAGCGCGTGTAAGGCCCATTCAACTGGTGCACTTGCCAG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGTTCCCTCTCCATTCATTTCGACTGAT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAAGAAACGGAAACCGCTGACTGTCCAGCA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACAAGTGGCACACACTAGACCAAAAGCAG10.7299270072992701No Hit
CGCGCGACGTACAGCGCGTGTATCGGTAGAAACAAGGGTGTTTTTCTCAT10.7299270072992701No Hit
CGCCTGACGTACAGCGCGTGTAGCCAGGGGAAGATCAGCAAGAACAAAAG10.7299270072992701No Hit
TGCGTGACGTACAGCGCGGGTATGGATGATAAGCAAAAGCAGGTTGACAC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGTCGGTACAGATGCAATTGTCGTTCTAA10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGCAATGATGCAAATGGAGGAAAAGGATTTTC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAGACGGGAAAGTGTCTTTGGAGGAAAG10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAATGCCCCTAATTTTCACTAGAATCAGGA10.7299270072992701No Hit
CGAGTGACGTACAGCGCGTGTACCGCTCATTTATTCCTACGATATCTTGC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGAGGAGGAACTATTTTACAGCAGAAGT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGGCCAAGGAGGTGTCACTAAGCTA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGCGGACAAAACTCCCGCTATATCCTG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTGTTCTCAGATTTTATTTTGTTGGCTTTCT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTATCACAGACAAATGGCTACTACCACCAAT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGGAGGGGCAATAGTTGGAGAAATTTCAC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGACAAAAACATAATGGACTCCAAC10.7299270072992701No Hit
CGCGTCACGTACAGCGCGTGTAGGAGGAGCAATAGTTGGAGAAATTTCAC10.7299270072992701No Hit
CTCGTGACGTACAGCGCGTGTAAGAAGCCAGTTAAAAAACAATGCCAAGG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAACTTGCCTCTCTCTGCATCTTTGGTCAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGAGCCGAACGGATGGACTGGGACAGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAATGGCTCCCGCTATATCCTGACCACTC10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTACAGATGCAATTGTCGTTCTAAGTGTCTC10.7299270072992701No Hit
CGCCTGACGTACAGCGCGTGTAATGGTGAAATTTCTCCAACTATTGCTCC10.7299270072992701No Hit
CGCGCGACGTACAGCGCGTGTATGCTAGAGTCCCGCTTTCGTTTCATTAC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGGAAAAAGGGCAAAATTGAAACCGGCAATGG10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTACAGAGGAGAAATGAAAAGAGGCGGGAGC10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAACAACAGACACTATCAAGAGTTGGAGAA10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTACGACCCACAAAAACATATGCATCTGCAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGAGGGCCAATTATCTTTTGCCTAGGCA10.7299270072992701No Hit
CGCATGACGTACAGCGCGTGTAGTGGTCTGGATGCTGAACAAAACTCCCG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACGCGTGATCAGTAGAAACAAGGTTGTTT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGTGTCCAGTAATAGTTCGTTCTCCCTTC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAATGCGAGGTTCAACACTTCCCAGAAGGT10.7299270072992701No Hit
CGCGGGACGTACAGCGCGGGTATGAGGCTCTGAAAAAATAAAGAGATTCG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACAGAGATTCGCTTGCATAAATGTTATTT10.7299270072992701No Hit
CGCGTGACTTACAGCACGTGTAGAAGTAGAAACAAGGTCGTTTTTAAACA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGAGAGCAATTGGGACAGAAATTTGAG10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAGGAGGGGGAACTTCACCCTTAAAGACAG10.7299270072992701No Hit
CGCGGGGCGTACAGCGCGTGTACAGGAGAGAAAGCTAATGTGCTAATTGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTATATGGTTCTTGCTTTAATGTAATGACGG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAGAAGGGAAAGACACCCACGGTCGATT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTACAGAGAGGAAATAGAGATAACAACCCAC10.7299270072992701No Hit
CCCGTGACGTACAGCGCGTGTAGTGGCTGTGTTAAAGTACAACGGCATAA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGCCACCATCCTCTCTATTGTTATGCTA10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGATGGTTCTTGCTTTACTGTAATGACCG10.7299270072992701No Hit
CGCATGACGTACAGCGCGTGTAACAGCAGCAAAGTTGGTGTTGCTGATGT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAAGGGACGAAGGACAAGGGTTGCTTATG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACCGGGGAGAAGCAATTTTCTCGATTCAG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACTGAAAGCGAACCTCAGTGAAAGCCCTT10.7299270072992701No Hit
CGCCTGACGTACAGCGCGTGTATGTGGGTCCTCCTGTTTTCTTAGGGTCC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGCCAGGCAAACAATGGCGAAGATGCAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGGAGGAAAAAACAAAGTATACGAAACC10.7299270072992701No Hit
CCCGTGACGTACAGCGCGTGTAGGGAGGAAAAGGATTTTCATTCAAATAC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGAGAGGAAACTTCACCAATAGGACAGC10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGCACCAAAAATGAAGGCAATACTAGTAG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAATGCCCCTAACTTTCACTAGAATCAGGA10.7299270072992701No Hit
CGCGTGATGTACAGCGCGTGTAAAGAGCATTGGTCCGTAAAACAAGGTTT10.7299270072992701No Hit
CGCGGGACGTACAGCGCGTGTATTTTGGACATTTTCCAATTTTGATCGGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTTGCGATAGCCCTTCTTTTTAACTTGCCTC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGCAAAAATACTGACATCAGAGTCA10.7299270072992701No Hit
CGCATGACGTACAGCGCGTGTAGCAGAGGCAATTGTCGTTCTAAGTGTCT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAACCCGGACAACATGGAACGTTATTTCTC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAACCCTGGAGATTTCATCGATTATGATGA10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAAGGAGAGGAAGCGAATGGGCTAAGTATG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGAAAGCGAACCTCAGTGAAAGCCCTTA10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAAGGAGCAAATGGAGTAAAAGGATATGCT10.7299270072992701No Hit
CGCATGACGTACAGCGCGTGTAAAGGGGGCAAATGGAGTAAAAGGATTTT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGTGGTCCAGTATCGTCTAATGGAGCAAA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACCACTCATTTATTCCTACGATATCTTGC10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAGAGAACAAAAGCGCTTGTTCGCACCAGA10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAACAACGACAATTGCATCTGTACCTACTT10.7299270072992701No Hit
CGCCTGACGTACAGCGCGTGTATAGTTTAACTGCTTTGTCCAAAATGCCC10.7299270072992701No Hit
CGCTTGACGTACAGCGCGTGTAGGTGGTTAATTGAAGAAATGCGGCACAG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAATAACCGACACTATCAAGAGTTGGAGAA10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAATGGTGAAATTTCTCCAACTATTGCTCC10.7299270072992701No Hit
CGCGTGACGGACAGCGCGTGTAGTGAAGGTCTCCCATTCTCATCACAGTT10.7299270072992701No Hit
CTCGTGACGTACAGCGCGTGTACATCTTCTTGGTCATGTTGTCTCTTACT10.7299270072992701No Hit
TGCGTGACCTACAGCGCGTGTAGAGCAGCCAATTGCTGAGGCAATAATTG10.7299270072992701No Hit
CGCGTGACGTAAAGAGCGTGTAGAGAGGGGAATTGCCCGCTAATTTCACG10.7299270072992701No Hit
AGCGTGACGTACAGCGCGTGTAAGGAGCAAATGGAGTAAAAGGATTTTCA10.7299270072992701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACA100.094.999997
GTACAGC100.094.999999
GACGTAC100.094.999996
CGTACAG100.094.999998
GTGACGT100.094.999994
CGTGACG100.094.999993
TGACGTA100.094.999995
Produced by FastQC (version 0.11.5)