FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC160CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC160CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5391
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGACGTACACTCTAGTATCCTGACCACTCATTTATTCCTACGATATC1212.244481543312929No Hit
CGCTGACGTACACTCTAGTATCCTACGATATCTTGCTTTATTGAGAAGTT901.669449081803005No Hit
CGCTGACGTACACTCTAGTATCATGACCACTCATTTATTCCTACGATATC551.0202188833240586No Hit
CGCTGACGTACACTCTAGTATCCTGAGCACTCATTTATTCCTACGATATC450.8347245409015025No Hit
CGCTGACGTACACTCTAGTATCCTGCCCACTCATTTATTCCTACGATATC370.6863290669634576No Hit
CGCTGACGTACACTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGA360.667779632721202No Hit
CGCTGACGTACACTCTAGTATCCGGACCACTCATTTATTCCTACGATATC350.6492301984789464No Hit
CGCTGACGTACACTCTAGTATCCTGCGATATCTTGCTTTATTGAGAAGTT330.6121313299944352No Hit
CGCTGACGTACACTCTAGTATCCTGATCACTCATTTATTCCTACGATATC230.42663698757187907No Hit
CGCTGACGTACACTCTAGTATCCCGCTATATCCTGACCACTCATTTATTC200.3709886848451122No Hit
CGCTGACGTACACTCTAGTATCCAGACCACTCATTTATTCCTACGATATC200.3709886848451122No Hit
CGCTGACGTACACTCTAGTATCCTACGCTATCTTGCTTTATTGAGAAGTT170.3153403821183454No Hit
CGCTGACGTACACTCTAGTATCTTGATGTCCCAACAAAGTCTCTATCAGA160.2967909478760898No Hit
CGCTGACGTACACTCTAGTATCCTGCCACTCATTTATTCCTACGATATCT150.27824151363383415No Hit
CGCTGACGTACACTCTAGTATCCGACCACTCATTTATTCCTACGATATCT150.27824151363383415No Hit
CGCTGACGTACACTCTAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGA130.24114264514932293No Hit
CGCTGACGTACACTCTAGTATCCCGACCACTCATTTATTCCTACGATATC130.24114264514932293No Hit
CGCTGACGTACACTCTAGTATCATACGATATCTTGCTTTATTGAGAAGTT130.24114264514932293No Hit
CGCTGACGTACACTCTAGTATCCTGAACACTCATTTATTCCTACGATATC120.22259321090706735No Hit
CGCTGACGTACACTCTAGTATCCTTCGATATCTTGCTTTATTGAGAAGTT110.20404377666481174No Hit
CGCTGACGTACACTCTAGTATCCCGACCAGTGAGTACCCTTCCCTTTCAA110.20404377666481174No Hit
CGCTGACGTACACTCTAGTATCGGGGGGCAAATGGAGTAAAAGGATTTTC110.20404377666481174No Hit
CGCTGACGTACACTCCAGTATCGTCTAATGGAGCAAATGGAGGAAAAGGA110.20404377666481174No Hit
CGCTGACGTACACTCTAGTATCCGACGATATCTTGCTTTATTGAGAAGTT100.1854943424225561No Hit
CGCTGACGTACACTCTAGTATCCAGAGCAGAAATGAAAAGTGGCGAGAGC100.1854943424225561No Hit
TGCTGACGTACACTCTAGTATCCTACGATATCTTGCTTTATTGAGAAGTT100.1854943424225561No Hit
CGCTGACGTACACTCCAGTCTCGGGTCAGTGAGTGAGAACTCCATACTTA100.1854943424225561No Hit
CGCTGACGTACACTCTAGTATCCCTCCCAGCGACTGAACCCCATGCACCA90.1669449081803005No Hit
CGCTGACGTACACTCTAGTATCATGCGAACAATTCAACAGACACTGTAGA90.1669449081803005No Hit
CGCTGACGTACACTCTAGTATCGAAACGAGAAAGCTCTTATCTCTTGTTC80.1483954739380449No Hit
CGCTGACGTACACTCTAGTATCATTCGCTTCTGGTAGGCCTGCAAATTTT80.1483954739380449No Hit
CGCTGACGTACACTCTAGTATCATTAAATAAGCTGAAACGAGAAAGCTCT80.1483954739380449No Hit
CGCTGACGTACACTCCAGTATCGTCTAATGGAGCAAATGGGGGAAAAGGA80.1483954739380449No Hit
CGCTGACGTACACTCTAGTATCTCCCGCTATATCCTGACCACTCATTTAT70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCTTCTCCTATTTCATCAAGTTCTATCCAG70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCCAGCATTTCTTTCCATTGCGAATGCATA70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCAGGCACTCCTTCCGTAGAAGGCCCTCTT70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCAGTAGCCCTGCAGTGGGACACTTCTGCT70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCCTCCGATATCTTGCTTTATTGAGAAGTT70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCATGCCCACTTGCTACTGCAAGCCCATAC60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCCTAACCCTTAAAAATCGGTCAATACTCA60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCGGGGCAACATCTTCTCCTATTTCATCAA60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCAAACGAGAAAGCTCTTATCTCTTGTTCT60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCAACCACCTGGAAAAAAGAATAGAGAATT60.11129660545353368No Hit
CGCCGACGTACACTCTAGTATCCTGACCACTCATTTATTCCTACGATATC60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCCTGAGCATGGCACCCATAATGTTCTCAA60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCCAGCCAGCAATGTTACATTTACCCAAAT60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCAACTCTCCTCCTTATTTTAACTTGATTT60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCTTGCTTTATTGAGAAGTTATTGTCTGTC60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCGAGGGGCAAATGGAGTAAAAGGATTTTC60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCAAGGGAGCAAATGGAGTAAAAGGATTTT60.11129660545353368No Hit
TGCTGACGTACACTCTAGTATCGGGGGAGCAAATGGAGTAAAAGGATTTT60.11129660545353368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT201.589386E-595.000018
CCTACAC201.589386E-595.000017
TGACATA201.589386E-595.000014
TGCTGAC450.095.000011
CTGACAT201.589386E-595.000013
GACATAC201.589386E-595.000015
ACATACA201.589386E-595.000016
CGTACAC4550.095.000017
GCTGACA201.589386E-595.000012
ATACACT201.589386E-595.000018
CATACAC201.589386E-595.000017
CGCTGAC4350.095.01
GTTGACG156.2606455E-495.02
ACGTACA4650.095.06
GTACACT4500.095.08
CTGACCT156.2606455E-495.03
TACACTC4850.095.09
GACGTAC4700.095.05
TTGACGT156.2606455E-495.03
GCTGACG4500.095.02
Produced by FastQC (version 0.11.5)