FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC154CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC154CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGGGTTTT130.4300363876943433No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTATTTT100.33079722130334105No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGTCGTTTT100.33079722130334105No Hit
CGTCACGTCGTGTGCGTGCTACGGCTGGTCCATACACACAGGCAGGCAGG80.2646377770426729No Hit
CGTCACGTCGTGTGCGTGCTACATCCAAATGTGCACCGAGCTTAAGCTCA80.2646377770426729No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGTAGTTTT70.23155805491233872No Hit
CGTCACGTCGTGTGCGTGCTACAAGAGCTCTTGTCCTCTGGTAAGTTGTA60.19847833278200464No Hit
CGTCACGTCGTGTGCGTGCTACAAGGGGGGAAGTTCACATATACTATCTG60.19847833278200464No Hit
CGTCACGTCGTGTGCCTGCTGCTCCAGATCTTCTAGGGAGAGTTGAACCT60.19847833278200464No Hit
CGTCACGTCGTGTGCGTGCTACCCGGGAACAACTAGGTCAGAAGTTTGAA60.19847833278200464No Hit
CGTCACGTCGTGTGCGTGCTACCAGCCGTTGCATCATCACCATTGTTGGC60.19847833278200464No Hit
CGTCACGTCGTGTGCGTGCTACAACACGAAAACGTTCTAATCTCTTGTTC60.19847833278200464No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTGTTTT50.16539861065167052No Hit
TGTCACGTCGTGTGCGTGCTACGGCTGGTCCATACACACAGGCAGGCAGG50.16539861065167052No Hit
CGTCACGTCGTGTGCGTGCTACAATCCCAATTATACTTGCGGCAACAACA50.16539861065167052No Hit
CGTCACGTCGTGTGCGTGCTACGGGGGGAGAATGGAAGAAAAACAAGGAT40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACAGTAGAAACAAGGGTATTTTTCATTAAT40.13231888852133644No Hit
TGTCACGTCGTGTGCGTGCTACAATCCCAATTATACTTGCGGCAACAACA40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACGAGGGAAGAACATCAGACATGAGGGCAG40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACAAGGGAACAACTAGGTCAGAAGTTTGAA40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACGAGGGCAGAAGTTTGAAGAAGTAAGATG40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACCAGATCTTCTAGGGAGAGTTGAACCTTG40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACTGATCCTCTCAATATGAGTGCAGACCGT40.13231888852133644No Hit
CATCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTGTTTT40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACATCAACAGTCACTGGATTGCAGCTGCCC40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACTTGATATTGTGGATTATTGATCGCCTTT40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACATTTGGATGTAGAATCGCCCAATTCCAC40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACATCCCCTTGCCCAATTAGCACATTAGCT40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACAGCCCCCATCCTGTTGTATATGAGTCCC40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACAACATCCTGTTTCACTGAGAAATCACTG40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACAACAAAGAAAAAGAAGTCCTTGTACTAT40.13231888852133644No Hit
TGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTGTTTT40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACAGTAGAAACAAGGGGATTTTTCATTAAT40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACATCCAAATCCGCCTTATTTCTTCTTTGT40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACGAGAGCTCAAAGACTCCCCGCCCCTGGA40.13231888852133644No Hit
CATCACGTCGTGTGCGTGCTACTGTCCATATTATTTGGATCCCCATTCCC40.13231888852133644No Hit
TGTCACGTCGTGTGCGTGCTACAAAAGGGGAAGGTTACTAAATCAATAGA40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACCCTGACCAATCAGTTATTGCTACAACAT40.13231888852133644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT2850.095.000017
CACGTCG2850.095.000014
TCGTGTG2850.095.000018
CGTGTGC2850.095.000019
CGTCACG1850.095.01
GTCACGT2500.095.02
TGTCACG550.095.01
CATCACG155.98774E-495.01
TCACGTC2750.095.03
ATCACGT155.98774E-495.02
CGTCGTG2950.095.06
ACGTCGT3000.094.999995
GCGGGCT356.302798E-847.514-15
AACAAGG150.00983547847.536-37
TACAGCA150.00983547847.520-21
GAAACAA150.00983547847.534-35
TGCGTGC2500.047.512-13
AAACAAG150.00983547847.536-37
GCTACTT150.00983547847.518-19
GCGTGCT2500.047.514-15
Produced by FastQC (version 0.11.5)