FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC152CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC152CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13194
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTAGAGATATAGACGTAGACAGAGCAGTCAAATGTTATCTCCCTCTT4263.2287403365165988No Hit
CGCGTAGAGATATAGACGTAGAATGAGAAAATGTGAATGTGTGTCTTCTC2551.9326966803092316No Hit
CGCGTAGAGATATAGACGTAGATTTTTGGATAGTGAATAGTACTTGCCAC1180.894345914809762No Hit
CGCGTAGAGATATAGACTTAGAAAGGGGTTTGAGATGATTTGGGATCCTA620.469910565408519No Hit
CGCGTAGAGATATAGACGTAGAGAGGATTCTTCTTTCAGAATCCTCTCTA470.35622252539032895No Hit
CGCGTAGAGATATAGACGTAGAAAAACCCATCACTCGGTCTGATCCGGCA420.3183265120509322No Hit
CGCGTAGAGATATAGACATAGTTTTCCATTGTGACTGTTCTCAAGCAGGT390.29558890404729427No Hit
CGCGTAGAGATATAGACGTAGAAGGCGCTCTCTTTAAACACGGTTTAAAA350.26527209337577684No Hit
CGCGTAGAGATATAGACGTAGAATGGACTGATAACACAGTTCGAATCTCT310.2349552827042595No Hit
CGCGTAGAGATATAGACGTAGACTGCTCTGTCCATATTATTTGGATCCCC290.21979687736850081No Hit
CGCGTAGAGATATAGACGTAGATTTTGGATAGTGAATAGTACTTGCCACT280.2122176747006215No Hit
CGCGTAGAGATATAGACGTAGACAGAGCAGTCAAACGTTATCTCCCTCTT260.19705926936486284No Hit
CGCGTAGAGATATAGACGTAGAAATAGCACTCAGTTATTCGGTGGTCACA240.18190086402910413No Hit
CGCGTAGAGATATAGACGTAGAAGGCTCTCTCTTTAAACACGGTTTAAAA230.1743216613612248No Hit
CGCGTAGAGATATAGACGTAGAATGAAATGATAACACAGTTCGAATCTCT220.16674245869334545No Hit
CGCGTAGAGATATAGACGTAGACGTACAATGGCAAATACTGTTTACAACT210.1591632560254661No Hit
CGCGTAGAGATATAGACGTAGAATCATCACATATGTGTGATTCACCCAGG200.1515840533575868No Hit
CGCGTAGAGATATAGACGTAGAAGGCAATGGTGAAATTTCGCCAACAATT190.14400485068970745No Hit
CGCGTAGAGATATAGACGTAGAAAAAGCAATGAAAGAGTATGGAGAGGAC190.14400485068970745No Hit
CGCGTAGAGATATAGACGTAGAAAGCCCTTAGTAATGTCAGATTCTCCAA190.14400485068970745No Hit
CGCGTAGAGATATAGACGTAGAATAAGCTGAAACGAAAACGTTCTAATCT180.1364256480218281No Hit
CGCGTAGAGATATAGACGTAGAATGAGCAAATGTGAATGTGTGTCTTCTC180.1364256480218281No Hit
CGCGTAGAGATATAGACGTAGAGGGAGCAATTGCTCCCTCTTCGGTGAAA180.1364256480218281No Hit
CGCGTAGAGATATAGACGTAGAGCTGTCAAACTTTATCTCCCTCTTAAGC180.1364256480218281No Hit
CGCGTAGAGATATAGACGTAGAAGGCCCTCTTTTTAAACCGTGTTTAAAG180.1364256480218281No Hit
CGCGTAGAGATATAGACGTAGAAACAAAGTAGTTTTTTACTCTAGCTCTA160.12126724268606943No Hit
CGCGTAGAGATATAGACGTAGACCCAGAAGCAAGGGCTTATACAGTCCAA160.12126724268606943No Hit
CGCGTAGAGATATAGACGTAGACCAAGCAAAAAGAGGGCCAGCCACTTTT160.12126724268606943No Hit
CGCGTAGAGATATAGACGTAGAATGCCAGATCATAATGTGAGTCAAACCA160.12126724268606943No Hit
CGCGTAGAGATATAGACGTAGAATGGTTCTGGCCTGACTAGCAACCTCCA160.12126724268606943No Hit
CGCGTAGAGATATAGACGTAGAATGGGATGATAACACAGTTCGAATCTCT160.12126724268606943No Hit
CGCGTAGAGATATAGACGTAGACAGAGAATAGTTTTGAGCAAATAATAGC150.11368804001819009No Hit
CGCGTAGAGATATAGACGTAGAAACAAGGGGGTTTTTTATCACTAAATAA150.11368804001819009No Hit
CGCGTAGAGATATAGACGTAGATGGGCTATATACACAAAAACATCTCCTT150.11368804001819009No Hit
CGCGTAGAGATATAGACGTAGACAGAGCAGTCAAACTTTATCGAAAGCTT150.11368804001819009No Hit
CGCGTAGAGATATAGACGTAGAAATAGCACCAGCAGAATAACTGAGTGCT150.11368804001819009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG12450.095.000019
GCGGAGA407.2759576E-1295.000012
CGCGGAG407.2759576E-1295.000011
GAGAGAT407.2759576E-1295.000015
TGCGTAG301.139415E-895.01
AGATATA12350.095.08
GAGATAT12400.095.07
CGCGTAG11650.094.999991
AGAGATA12450.094.618486
TAGAGAT12050.094.605815
GCGTAGA11850.094.198312
GTAGAGA13100.086.66034
CGTAGAG15150.074.306933
AGAGATT200.00202863971.256
GAGATTT200.00202863971.257
GGAGAGA551.3278623E-1069.090914
CGGAGAG551.3278623E-1069.090913
GATTTAG250.00491188757.09
GTAGATG205.29214E-447.50000418-19
TAGAACG403.95994E-947.50000418-19
Produced by FastQC (version 0.11.5)