FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC149CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC149CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310653
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATAGACGACTAGTACGTGAATGAGAAAATGTGAATGTGTGTCTTCTC61751.9877483880728657No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGGTGTTTTTTATCA23850.7677376365269287No Hit
CGTATAGACGACTAGTACGTGAAGGACCAAAAGTAACAGACTTAGAAAGG20520.6605440797288292No Hit
CGTATAGACGACTAGTACGTGAATGAGCAAATGTGAATGTGTGTCTTCTC16020.5156879218935597No Hit
CGTATAGACGACTAGTACGTGACCCAGAAGCAAGGTCTTATACAGTCCAA15530.49991469581816367No Hit
CGTATAGACGACTAGTACGTGACGCCACAAAAAGAAATGCTGCTCCCACT14530.46772443852143714No Hit
CGTATAGACGACTAGTACGTGAATGTGTGTCTTCTCAGATTTAATTTTGT11960.3849954772688498No Hit
CGTATAGACGACTAGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTAT11850.3814545489662099No Hit
CGTATAGACGACTAGTACGTGATTCACCCAGGTGCTGTTTTCATATGTGA11720.37726981551763544No Hit
CGTATAGACGACTAGTACGTGAACACAAATCCTAAAATCCCCTTAGTCAG11260.3624622971611412No Hit
CGTATAGACGACTAGTACGTGAGGGGCGGGAACAACTAGGTCAGAAGTTT10630.3421824350642035No Hit
CGTATAGACGACTAGTACGTGACAGAGCAGTCAAATGTTATCTCCCTCTT10200.32834062442661105No Hit
CGTATAGACGACTAGTACGTGAAAGAAATTTCAGTGTCCTGGGAGTTGGT10040.3231901832591348No Hit
CGTATAGACGACTAGTACGTGACCGAGAGTGCTGCCTCTTCCCTTTAGAG8930.2874589976597683No Hit
CGTATAGACGACTAGTACGTGAAACAGGATGTTGGACGAAACTTCCGCTG8480.27297338187624137No Hit
CGTATAGACGACTAGTACGTGACTTAGAAAGGGGTTTGAGATGATTTGGG8260.2658915252709615No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGTAGTTTTTTACTC7880.2536592274982054No Hit
CGTATAGACGACTAGTACGTGACTTCCAGTTTGGATTGAGTGACTAGCCC7730.2488306889036964No Hit
CGTATAGACGACTAGTACGTGAAAACCCTTTTACTCCGTTTGCTCCATCA7630.24561166317402372No Hit
CGTATAGACGACTAGTACGTGAAAGGGGTTTGAGATGATTTGGGATCCTA7310.23531078083907125No Hit
CGTATAGACGACTAGTACGTGAACGCCACAAAAAGAAATGCTGCTCCCAC7300.23498887826610398No Hit
CGTATAGACGACTAGTACGTGAGGGGCCGCAAGTATAATTGGGATTGTGC6540.21052428272059176No Hit
CGTATAGACGACTAGTACGTGTGATCAGTAGAAACAAGGGTGTTTTTTAT6460.20794906213685369No Hit
CGTATAGACGACTAGTACGTGAAGGGCGGGAACAACTAGGTCAGAAGTTT6380.20537384155311553No Hit
CGTATAGACGACTAGTACGTGACGCGTGATCAGTAGAAACAAGGGTGTTT6280.20215481582344288No Hit
CGTATAGACGACTAGTACGTGAACCCAAATCAAAAGATAATAACCATTGG6210.19990149781267202No Hit
CGTATAGACGACTAGTACGTGATTGTGCACTTGATATTGTGGATTATTGA5960.1918539334884904No Hit
CGTATAGACGACTAGTACGTGACCGCCATTTTCCGTTTCTGTGTTGTAGT5610.18058734343463606No Hit
CGTATAGACGACTAGTACGTGACAGCCCATATTGAAATAATATTTCCTAT5540.1783340254238652No Hit
CGTATAGACGACTAGTACCAGAGTCTATGAGGGAAGAATATCGAGAGGAA5320.17125216881858535No Hit
CGTATAGACGACTAGTACGTGAACAAGCAGCTGAGGCCATGGAGGTTGCT5240.16867694823484725No Hit
CGTATAGACGACTAGTACGTGATGATTCTTTGGTTGCATATTCCAGTGTT5190.16706743537001092No Hit
CGTATAGACGACTAGTACGTGACTAGCCCATATTGAAATAATATTTCCTA5180.16674553279704363No Hit
CGTATAGACGACTAGTACGTGACTGCCCATATTGAAATAATATTTCCTAT5090.16384840964033826No Hit
CGTATAGACGACTAGTACGTGAATAAGCTGAAACGAAAACGTTCTAATCT5080.16352650706737099No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGGGGTTTTTTATCA5010.16127318905660012No Hit
CGTATAGACGACTAGTACGTGACATGGCTGGCAATTCTTATGCTGTTGTC4900.1577322607539602No Hit
CGTATAGACGACTAGTACGTGATTTGGGATCCTAATGGATGGACAAATAC4880.15708845560802567No Hit
CGTATAGACGACTAGTACATGAAGCACACTTCCAAGTGGGTGCATATTGC4170.13423337292734983No Hit
CGTATAGACGACTAGTACGTGATGGGGGTGCAGATGCAACGATTCAAGTG4150.13358956778141529No Hit
CGTATAGACGACTAGTACGTGAAAGCCCTTAGTAATGTCAGATTCTCCAA4050.13037054205174262No Hit
CGTATAGACGACTAGTACGTGACAGAGCAGTCAAACTTTATCGAAAGCTT4040.13004863947877535No Hit
CGTATAGACGACTAGTACGTGAACCCGAATCAAAAGATAATAACCATTGG4010.12908293175987356No Hit
CGTATAGACGACTAGTACGCGTGATCAGTAGAAACAAGGTAGTTTTTTAC3960.12747341889503722No Hit
CGTATAGACGACTAGTACGTGACGGAGCTTCACCTAGAGGACAGCTCATT3900.12554200345723363No Hit
CGTATAGACGACTAGTACGTGACATGGGCTGGCAATTCTTATGCTGTTGT3880.12489819831129911No Hit
CGTATAGACGACTAGTACGTGAAGGGGGGGAACAACTAGGTCAGAAGTTT3870.12457629573833183No Hit
CGTATAGACGACTAGTACGTGAAGGCCCTCTTTTTAAACCGTGTTTAAAG3850.1239324905923973No Hit
CGTATAGACGACTAGTACGTGAAGACACTACAGAGACATAAGCATTTTCT3730.12006965971679011No Hit
CGTATAGACGACTAGTACGTGATCATGGCCAAGAACATTCTAGGGTTTTG3640.11717253656008474No Hit
CGTATAGACGACTAGTACGTGAATGCCAGATCATAATGTGAGTCAAACCA3620.1165287314141502No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGTTGTTTTTTATCA3620.1165287314141502No Hit
CGTATAGACGACTAGTACGTGATTTCTCAGTGAAACAGGATGTTGTAGCA3480.11202209539260848No Hit
CGTATAGACGACTAGTACGTGACACCCAAGGTCGATTTGAACCATGCCAG3420.11009067995480488No Hit
CGTATAGACGACTAGTACGTGACCCAAATGCACTTTTAAAGCACAGATTT3330.10719355679809948No Hit
CGTATAGACGACTAGTACGTGAACAATCTGGTTTTAATCCTAGCTCTGCT3180.10236501820359051No Hit
CGTATAGACGACTAGTACGTGACGCGTGATCAGTAGAAACAAGGTAGTTT3170.10204311563062324No Hit
CGTATAGACGACTAGTACGTGACGGGCGGGAACAACTAGGTCAGAAGTTT3170.10204311563062324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAC201.7418182E-595.000013
CGTATTG500.095.000011
CGTATCG201.7418182E-595.000011
GTACAGA500.095.000012
TTGACGA500.095.000015
AACGACT409.094947E-1295.000017
GTATCGA201.7418182E-595.000012
ACAGACG500.095.000014
CCGACTA254.612666E-795.000018
TAGGCGA450.095.000015
CGACTTG254.612666E-795.000019
CGACTGG254.612666E-795.000019
CCTATAG409.094947E-1295.000011
TATCGAC201.7418182E-595.000013
ACGACTG254.612666E-795.000018
ACGCCTA600.095.08
CGACGAG156.6133414E-495.09
TAGACGT301.2289092E-895.05
GACGTCT301.2289092E-895.07
CGCATAG550.095.01
Produced by FastQC (version 0.11.5)