FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC139CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC139CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66528
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGTAGATACGACAGAGAGACAGAGCAGTCAAATGTTATCTCCCTCTT15012.256192881192881No Hit
CGTAGTAGATACGACAGAGAGAATGAGAAAATGTGAATGTGTGTCTTCTC6030.9063852813852814No Hit
CGTAGTAGATACGACAGAGAGAAGTCAAACTTTATCTCCCTCTTAAGCTT4430.665885040885041No Hit
CGTAGTAGATACGACAGAGAGATGAATTGCCAGCCAATGTCACTGAAGTT3510.5275974025974026No Hit
CGTAGTAGATACGACAGAGAGACGCCACAAAAAGAAATGCTGCTCCCACT2820.4238816738816739No Hit
CGTAGTAGATACGACAGAGAGAGGGGTTTGAGATGATTTGGGATCCTAAT2600.39081289081289083No Hit
CGTAGTAGATACGACAGAGAGACTGCTCTGTCCATATTATTTGGATCCCC2450.3682659932659933No Hit
CGTAGTAGATACGACAGAGAGAATGCCAGATCATAATGTGAGTCAAACCA2340.35173160173160173No Hit
CGTAGTAGATACGACAGAGAGAGGGGGCCTGACGGGATGATAGAGAGAAC2240.33670033670033667No Hit
CGTAGTAGATACGACAGAGAGAAGTAGAAACAAGGTAGTTTTTTACTCTA1850.2780784030784031No Hit
CGTAGTAGATACGACAGAGAGAGGATTCTTCTTTCAGAATCCTCTCTACT1750.2630471380471381No Hit
CGTAGTAGATACGACAGAGAGAAGGGGTTTGAGATGATTTGGGATCCTAA1730.260040885040885No Hit
CGTAGTAGATACGACAGAGAGATTTTTGGATAGTGAATAGTACTTGCCAC1570.23599086099086097No Hit
CGTAGTAGATACGACCGAGAGTGCTGCCTCTTCCCTTTAGAGACTTCTGA1550.232984607984608No Hit
CGTAGTAGATACGACAGAGAGAAATCACTGTCGGTATTTGTCCATCCATT1490.22396584896584898No Hit
CGTAGTAGATACGACAGAGAGAGGGGGTGCAGATGCAACGATTCAAGTGA1440.21645021645021645No Hit
CGTAGTAGATACGACAGAGAGAAGGACCAAAAGTAACAGACTTAGAAAGG1320.1984126984126984No Hit
CGTAGTAGATACGACAGAGAGAAGGCAATGGTGAAATTTCGCCAACAATT1300.19540644540644542No Hit
CGTAGTAGATACGACAGAGAGACAGAGCAGTCAAACGTTATCTCCCTCTT1280.1924001924001924No Hit
CGTAGTAGATACGACAGAGAGAACCAGCCGTTGCATCATCACCATTGTTG1250.18789081289081289No Hit
CGTAGTAGATACGACAGAGAATAGTTTTGAGCAAATAACATTTATGCAAG1230.18488455988455987No Hit
CGTAGTAGATACGACAGAGAGAAGGCGATCAATAATCCACAATATCAAGT1220.1833814333814334No Hit
CGTAGTAGATACGACAGAGAGAAAGGGGTTTGAGATGATTTGGGATCCTA1220.1833814333814334No Hit
CGTAGTAGATACGACAGAGAGAAAGCCCTTAGTAATGTCAGATTCTCCAA1180.17736892736892737No Hit
CGTAGTAGATACGACAGAGAGATGGGGGTGCAGATGCAACGATTCAAGTG1180.17736892736892737No Hit
CGTAGTAGATACGACAGAGAGAACGCCACAAAAAGAAATGCTGCTCCCAC1130.16985329485329487No Hit
CGTAGTAGATACGACAGAGAGAGGGAGATAACATTCCAAGTGCACCAGCA1100.16534391534391535No Hit
CGTAGTAGATACGACAGAGAGAGGGAGCAATTGCTCCCTCTTCGGTGAAA1090.16384078884078884No Hit
CGTAGTAGATACGACAGAGAGAGGGCAGCTGCCCTCTCCATCTTCGGGAC1080.16233766233766234No Hit
CGTAGTAGATACGACAGAGAGACTTAGAAAGGGGTTTGAGATGATTTGGG1030.1548220298220298No Hit
CGTAGTAGATACGACAGAGAGACAGCCATTTGCTCCATAGCCTTAGCTGT1020.15331890331890333No Hit
CGTAGTAGATACGACAGAGAGAAGTAGAAACAAGGGTGTTTTTTATCACT980.1473063973063973No Hit
CGTAGTAGATACGACAGAGAGAGTGGGGGTGCAGATGCAACGATTCAAGT970.1458032708032708No Hit
CGTAGTAGATACGACAAAGAGATGAATTGCCAGCCAATGTCACTGAAGTT920.1382876382876383No Hit
CGTAGTAGATACGACAGAGAGAACAAGCAGATGAGTCCCAATGGCTCTCA910.1367845117845118No Hit
CGTAGTAGATACGACAGAGAGAGAGGGGTTTGAGATGATTTGGGATCCTA890.13377825877825877No Hit
CGTAGTAGATACGACAGAGAGAGTCAAACTTTATCTCCCTCTTAAGCTTT880.13227513227513227No Hit
CGTAGTAGATACGACAGAGAGACGGAGCTTCACCTAGAGGACAGCTCATT870.13077200577200576No Hit
CGTAGTAGATACGACAGAGAGACCCAGAAGCAAGGTCTTATACAGTCCAA860.12926887926887926No Hit
CGTAGTAGATACGACAGAGAGAGGGGGCAATGGTGAAATTTCGCCAACAA840.12626262626262627No Hit
CGTAGTAGATACGACAGAGAGATTTGGGATCCTAATGGATGGACAAATAC820.12325637325637326No Hit
CGTAGTAGATACGACAGAGAGAAGGCCCTCTTTTTAAACCGTGTTTAAAG820.12325637325637326No Hit
CGTAGTAGATACGACAGAGAGACCGAGAGTGCTGCCTCTTCCCTTTAGAG820.12325637325637326No Hit
CGTAGTAGATACGACAGAGAGAGGGGGCTGTGACCACCGAATCAGCATTT800.12025012025012026No Hit
CGTAGTAGATACGACAGAGAGAAATAGCACTCAGTTATTCGGTGGTCACA760.11423761423761424No Hit
CGTAGTAGATACGACAGAGAGAATGTGAATGTGTGTCTTCTCAGATTTAA760.11423761423761424No Hit
CGTAGTAGATACGACAGAGAGACGAAAACGTTCTAATCTCTTGTTCCACT740.11123136123136124No Hit
CGTAGTAGATACGACAGAGAGACGCGTGATCAGTAGAAACAAGGGTGTTT720.10822510822510822No Hit
CGTAGTAGATACGACAGAGAGAACAGGATGGTTCTGGCCTGACTAGCAAC710.10672198172198173No Hit
CGTAGTAGATACGACAGAGAGAACAAGCAGCTGAGGCCATGGAGGTTGCT710.10672198172198173No Hit
CGTAGTAGATACGACAGAGAGAGGGGGATAACATTCCAAGTGCACCAGCA710.10672198172198173No Hit
CGTAGTAGATACGACAGAGAGAACAAGCAGCTGCTTGTTCGCTCGATCCA700.10521885521885521No Hit
CGTAGTAGATACGACAGAGAGACGCGTGATCAGTAGAAACAAGGTAGTTT700.10521885521885521No Hit
CGTAGTAGATACGACAGAGAGAGGGGGGCTGTGACCACCGAATCAGCATT700.10521885521885521No Hit
CGTAGTAGATACGACAGAGAGAGGGGGATAACATTCCATGAGTCCCATAC690.10371572871572872No Hit
CGTAGTAGATACGACAGAGATTCGCTTGGAGAAGCAGTAATGAGACTGGG680.10221260221260221No Hit
CGTAGTAGATACGACAGAGAGACTTCAAATAATAGCTGTAATGCTTGCAT670.10070947570947572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA201.7317205E-595.000013
ATAGGAC201.7317205E-595.000019
CGTGGTA201.7317205E-595.000011
CGTAATA201.7317205E-595.000011
GTGGTAG201.7317205E-595.000012
GTAATAG201.7317205E-595.000012
TAGATAG201.7317205E-595.000016
CGTAGGA156.5903016E-495.01
CGTAGCA156.5903016E-495.01
GTAGGAG156.5903016E-495.02
AGGTACG156.5903016E-495.07
GGTACGA156.5903016E-495.08
ATACGAC65100.094.562219
GTAGTAG65100.094.489252
CGTAGTA64900.094.487671
TAGATAC65150.094.416736
TAGTAGA65350.094.345833
GATACGA65350.094.200468
AGATACG65500.094.129777
AGTAGAT65500.093.912224
Produced by FastQC (version 0.11.5)